中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (36): 6701-6704.doi: 10.3969/j.issn.1673-8225.2011.36.012

• 脐带脐血干细胞 umbilical cord blood stem cells • 上一篇    下一篇

脐带间充质干细胞对CIA大鼠炎症因子的干预

李  慧1，顾  健2，林传明2，马  莉2，沈连军2，吴  蔚2，汪中强2   

  1. 1扬州大学医学院，江苏省扬州市   225001
    2扬州大学临床医学院扬州市血液学研究所，江苏省扬州市   225001
  • 收稿日期:2011-03-06 修回日期:2011-04-11 出版日期:2011-09-03 发布日期:2011-09-03
  • 通讯作者: 顾健，教授，主任医师，扬州大学临床医学院扬州市血液学研究所，江苏省扬州市 225001 maolujiu918@yahoo.com.cn
  • 作者简介:李慧★，女， 1985年生，江苏省东海县人，汉族，2011年扬州大学毕业，硕士，主要从事血液风湿病学方面研究。 lh99beautiful@126.com
  • 基金资助:

    江苏省卫生厅立项课题(201048)。

Umbilical cord mesenchymal stem cells effect on inflammatory responses of rats with type Ⅱ collagen-induced arthritis

Li Hui1, Gu Jian2, Lin Chuan-ming2, Ma Li2, Shen Lian-jun2, Wu Wei2, Wang Zhong-qiang2   

  1. 1Clinical Medical College of Yangzhou University, Yangzhou  225001, Jiangsu Province, China
    2Institute of Hematology of Yangzhou, Clinical Medical College of Yangzhou University, Yangzhou  225001, Jiangsu Province, China
  • Received:2011-03-06 Revised:2011-04-11 Online:2011-09-03 Published:2011-09-03
  • Contact: Gu Jian, Professor, Chief physician, Institute of Hematology of Yangzhou, Clinical Medical College of Yangzhou University, Yangzhou 225001, Jiangsu Province, China maolujiu918@yahoo.com.cn
  • About author:Li Hui★, Master, Clinical Medical College of Yangzhou University, Yangzhou 225001, Jiangsu Province, China lh99beautiful@126.com
  • Supported by:

    the Health Bureau of Jiangsu Province, No. 201048*

PDF

391

被引次数

25

摘要：

背景：脐带间充质干细胞可表达多种胚胎干细胞的特有分子标志，具有分化潜力大、增殖能力强、免疫原性低等特征。
目的：观察脐带间充质干细胞对类风湿性关节炎免疫炎性的病理过程的干预作用。
方法：实验分为3组，建立Ⅱ型胶原诱导的类风湿性关节炎模型大鼠，脐带间充质干细胞组于造模后第4周经大鼠尾静脉注射脐带间充质干细胞，模型组注射生理盐水。
结果与结论：治疗后第7天及第35天模型组大鼠血清白细胞介素1、白细胞介素6、白细胞介素18、肿瘤坏死因子α、血管细胞黏附分子1和中性粒细胞表面CD11b的表达水平均高于正常组(P < 0.05)。脐带间充质干细胞治疗后各因子水平显著低于模型组(P < 0.05)，治疗后第35天显著低于第7天(P < 0.05)。表明脐带间充质干细胞能明显抑制CIA大鼠的炎症因子释放和抑制内皮细胞的异常活化，有利于缓解类风湿性关节炎的免疫炎性关节症状。

关键词: 脐带间充质干细胞, CIA大鼠, 炎症因子, 血管细胞黏附分子, 白细胞介素

Abstract:

BACKGROUND: Umbilical cord mesenchymal stem cells (UC-MSCs) can express various kinds of embryonic stem cell-specific molecular markers, and be characterized as good differentiation potential, strong proliferative capacity, and low immunogenicity.
OBJECTIVE: To observe the interventional effect of UC-MSCs on immune inflammatory pathological process of rheumatoid arthritis. 
METHODS: CIA rat models were established and divided into three groups. At 4 weeks after modeling, UC-MSCs were injected via the tail vein in the UC-MSCs group, and normal saline was injected in the model group.
RESULTS AND CONCLUSION: The levels of interleukin-1 (IL-1), IL-6, IL-18, tumor necrosis factor alpha, vascular cell adhesion molecule and neutrophil cell surface expression of CD11b were higher in the model group than the normal group (P < 0.05) at 7 and 35 days after treatment. Those factor levels in the UC-MSCs group were significantly lower than those in the model group after treatment (P < 0.05), those at 7 days were higher than at 35 days (P  < 0.05). UC-MSCs can obviously inhibit the release of inflammatory cytokines and abnormal activation of endothelial cells, and relieve immune and inflammatory reactions of rheumatiod arthritis.

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