中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (36): 6665-6668.doi: 10.3969/j.issn.1673-8225.2011.36.004

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

Notch信号系统调控骨髓间充质干细胞向心肌样细胞的分化

牛  萍1，赵月强2，黄星原1   

  1. 武汉大学人民医院，1儿科，2整形外科，湖北省武汉市  430060
  • 收稿日期:2011-03-17 修回日期:2011-05-16 出版日期:2011-09-03 发布日期:2011-09-03
  • 作者简介:牛萍☆，女，1978年生，山东省章丘市人，汉族，2007年武汉大学毕业，博士，主治医师，主要从事干细胞的基础与临床研究。 niuping999@163.com
  • 基金资助:

    国家自然科学基金(30801131)，课题名称：Notch信号调控起搏基因修饰的骨髓间充质干细胞构建生物起搏器的研究。教育部高等学校博士学科点专项科研基金(200804861045)，课题名称：Notch信号通路调控起搏基因修饰的骨髓间充质干细胞构建生物起搏器的研究。

Notch1 signaling system regulates the differentiation of bone marrow mesenchymal stem cells into cardiomyocytes

Niu Ping1, Zhao Yue-qiang2, Huang Xing-yuan1   

  1. 1Department of Pediatrics, 2Department of Plastic Surgery, Renmin Hospital of Wuhan University, Wuhan  430060, Hubei Province, China
  • Received:2011-03-17 Revised:2011-05-16 Online:2011-09-03 Published:2011-09-03
  • About author:Niu Ping☆, Doctor, Attending physician, Department of Pediatrics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China niuping999@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30801131*; the Doctoral Program of Higher Education of Ministry of Education, No. 200804861045*

PDF

301

被引次数

8

摘要：

背景：Notch信号系统在调控骨髓间充质干细胞的定向分化中起关键作用，但尚无涉及干细胞分化为心肌细胞及其分化机制的报道。
目的：分析Notch信号系统在骨髓间充质干细胞分化为心肌细胞过程中的调控作用。
方法：将分离培养的骨髓间充质干细胞与心肌细胞共培养，Jagged1组加入Notch激活剂Jagged1，DAPT组加入Notch激活剂Jagged1和抑制剂DAPT，对照组加入PBS缓冲液。用反转录-聚合酶链反应、免疫组化等方法检测干细胞分化为心肌细胞的情况及Notch信号系统的表达。
结果与结论：骨髓间充质干细胞在体外可分化为心肌细胞，与DAPT组及对照组相比，Jagged1组的干细胞分化为心肌细胞的比率提高，心肌标志物表达增多，并且Notch1和Jagged1表达增强。证实Notch信号系统对骨髓间充质干细胞分化为心肌细胞起正向调控作用。

关键词: 心肌细胞, Notch信号系统, 骨髓间充质干细胞, 分化, 调控

Abstract:

BACKGROUND: Notch signaling system has an important role of regulating the differentiation of bone marrow mesenchymal stem cells (BMSCs). However, there are no reports about Notch signaling system effect on differentiation and mechanism of BMSCs into cardiomyocytes.
OBJECTIVE: To investigate the regulation of differentiation of BMSCs into cardiomyocytes by Notch signaling system.
METHODS: BMSCs were cocultured with neonatal rat ventricular myocytes in vitro. Jagged1, an activator of the Notch signaling system, was added into the culture medium of the experiment group. The control group was added with Jagged1 and DAPT, an inhibitor of the Notch signaling system. The cells were cultured in the medium added with phosphate-buffered saline used as blank control group. Then the cultured cells were collected. The expression of Notch signal and TnT was detected by RT-PCR and immunohistochemistry.
RESULTS AND CONCLUSION: BMSCs can differentiate into cardiomyocytes in vitro. Compared with the two control groups, the differentiate percentage was higher in the experimental group. RT-PCR showed that the expression level of the receptor Notch1 and the ligand Jagged1 was significantly higher. Immunohistochemistry showed a significantly increased expression of TnT. When the Notch signaling system is activated, the differentiation of BMSCs into cardiomyocytes enhances, while the effect can be inhibited by DAPT.

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