中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (32): 6068-6071.doi: 10.3969/j.issn.1673-8225.2011.32.041

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

脂肪干细胞的体外诱导与分化

任逸众，韩长旭   

  1. 内蒙古医学院第二附属医院关节镜与运功医学外科，内蒙古自治区呼和浩特市010030
  • 收稿日期:2011-02-18 修回日期:2011-03-13 出版日期:2011-08-06 发布日期:2011-08-06
  • 作者简介:任逸众★，男，1974年生，内蒙古自治区临河市人，汉族，2004年内蒙古医学院毕业，硕士，副教授，主要从事运动医学方面的研究。 poceidonone@sohu.com
  • 基金资助:

    内蒙古自治区自然基金资助项目(2010MS1109)。项目名称：负载GDF-7基因的兔脂肪干细胞移植干预重建前交叉韧带腱骨界面愈合及止点改建塑形的实验研究。

In vitro induction and differentiation of adipose-derived stem cells

Ren Yi-zhong, Han Chang-xu   

  1. Department of Arthroscopy and Sports Medicine, the Second Hospital of Inner Mongolia Medical College, Hohhot  010030, Inner Mongolia Autonomous Region, China
  • Received:2011-02-18 Revised:2011-03-13 Online:2011-08-06 Published:2011-08-06
  • About author:Ren Yi-zhong★, Master, Associate professor, Department of Arthroscopy and Sports Medicine, the Second Hospital of Inner Mongolia Medical College, Hohhot 010030, Inner Mongolia Autonomous Region, China poceidonone@sohu.com
  • Supported by:

    the Natural Science Foundation of Inner Mongolia Autonomous Region, No. 2010MS1109*

PDF

385

被引次数

21

摘要：

背景：应用脂肪干细胞来作为组织工程的种子细胞是近年来组织工程研究中的一个活跃领域，大量的研究表明在不同的条件下脂肪干细胞可以向机体的不同组织分化。
目的：对国内外脂肪干细胞体外诱导分化的现状及新进展作一综述。
方法：应用计算机检索CNKI和PubMed数据库中2000-01/2010-10关于脂肪干细胞的文章，在标题和摘要中以“脂肪干细胞，体外分化潜能，种子细胞，转基因”或“Adipose-derived stem cells，cell differentiation in vitro，seeded cell，gene transfaction”为检索词进行检索。选择文章内容与脂肪干细胞体外分化有关者，同一领域文献则选择近期发表或发表在权威杂志文章。初检得到112篇文献，最终入选28篇文献进行综述。
结果与结论：脂肪干细胞是存在于脂肪组织中的具有高度自我更新能力和多向分化潜能的干细胞群体。它具有多向系分化潜能，除可以分化为间充质来源的脂肪、骨、软骨以及骨骼肌、心肌等细胞，也可诱导分化为来源于外胚层的神经细胞以及具有功能性的血管内皮细胞。此外脂肪干细胞还具有造血支持作用以及可被腺病毒等高度转染等优点，可以作为基因转移良好的靶细胞。

关键词: 脂肪干细胞, 体外分化潜能, 种子细胞, 转基因, 综述文献

Abstract:

BACKGROUND: For the past few years, the application of adipose derived stem cells (ADSCs) as seeded cells of tissue engineering is an active field in medications and ADSCs could differentiate into many tissues in vitro.
OBJECTIVE: To summarize the situation and progression about ADSCs induced in vitro at home and abroad.
METHODS: Databases of CNKI and PubMed (2000-01/2010-10) were selected to search the related articles about ADSCs using the keywords of “adipose-derived stem cells, cell differentiation in vitro, seeded cell, gene transfaction” in Chinese and English. There were 112 articles after the initial survey. Then 28 articles related to sustained-release antimicrobials were involved.
RESULTS AND CONCLUSION: ADSCs have extensive self-renewal capacity and the ability to differentiate along multiple lineages. With the multilineage differentiation potential, ADSCs can differentiate not only into such mesenchymal lineages as adipogenesis, chondrogenesis, osteogenesis, myogenesis, but also into neurogenesis of origin ectoderm. In addition, ADSCs have high infected efficiency by oncoretroviral vectors and have the ability to support hematopoiesis.

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