中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (32): 6032-6035.doi: 10.3969/j.issn.1673-8225.2011.32.032

• 干细胞与中医药 stem cells and traditional Chinese medicine • 上一篇    下一篇

人参皂苷Rg1干预骨髓间充质干细胞转化为多潜能干细胞关键基因mRNA的表达

李佳玮1，2，张密霞1，2，周  涛1，2，石田寅夫2，王秀云1，2   

  1. 1天津中医药大学，天津市  300193
    2铃鹿医疗科学大学东洋医学研究所，日本铃鹿  510-0293
  • 收稿日期:2011-02-26 修回日期:2011-06-01 出版日期:2011-08-06 发布日期:2011-08-06
  • 通讯作者: 王秀云，天津中医药大学针灸系，天津市 300193 wxyzhaq@126.com
  • 作者简介:李佳玮★，女，1981年生，河北省石家庄市人，汉族，硕士，实验师，主要从事细胞生物学研究。 lijiawei1981@163.com
  • 基金资助:

    日本国文部科学省高技术研究中心整备事业项目(No 16文科高第978号)，课题名称：从中西医两方面开展脑卒中及其后遗症、认知症、骨质疏松症、癌等防治法及机制研究。

Effects of Ginsenoside Rg1 on the expressions of Nanog, c-Myc, Oct, Klf4, Sox2 mRNA during the differentiation of bone marrow mesenchymal stem cells into pluripotent stem cells

Li Jia-wei 1, 2, Zhang Mi-xia 1, 2, Zhou Tao 1, 2, Torao Ishida2, Wang Xiu-yun 1, 2   

  1. 1Tianjin University of Traditional Chinese Medicine, Tianjin  300193, China
    2Institute of Traditional Chinese Medicine, Suzuka University of Medical Science, Suzuka 510-0293, Japan
  • Received:2011-02-26 Revised:2011-06-01 Online:2011-08-06 Published:2011-08-06
  • Contact: Wang Xiu-yun, Institute of Traditional Chinese Medicine, Suzuka University of Medical Science, Suzuka 510-0293, Japan; Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China wxyzhaq@126.com
  • About author:Li Jia-wei★, Master, Technician, Institute of Traditional Chinese Medicine, Suzuka University of Medical Science, Suzuka 510-0293, Japan; Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China lijiawei1981@163.com
  • Supported by:

    the “High-Tech Research Center” Project of Japan (MEXT.HAITEKU, 2006-2010. Project number: 05H018, 2005. 16 Japan Education Science High-Tech 978)*

PDF

437

被引次数

14

摘要：

背景：将成体细胞重编程为诱导多潜能干细胞方案主要通过反转录病毒将Oct-4, Sox2, c-Myc, Klf4等基因转入成体细胞而实现。
目的：观察人参皂苷Rg1作用于骨髓间充质干细胞后，对成体细胞向诱导多潜能干细胞转化的关键性基因Oct4、Sox2、c-Myc、Klf4、Nanog mRNA表达的影响。
方法：培养骨髓间充质干细胞，对照组培养基为α-MEM，体积分数5%FBS，1%双抗；用药组培养基为α-MEM，体积分数15%FBS，1 000 U/mL Rat ESGRO®，1%双抗，并加入6.25 μmol/L和12.5 μmol/L人参皂苷Rg1。检测骨髓间充质干细胞Oct4，Sox2，c-Myc，Klf4，Nanog等mRNA的表达。
结果与结论：人参皂苷Rg1 6.25 μmol/L培养30 d，Nanog、c-Myc、Oct、Klf4、Sox2 mRNA表达均有升高，且Nanog、c-Myc与对照组差异有显著性意义。人参皂苷Rg1能促进骨髓间充质干细胞表达c-Myc，Nanog，但Nanog阳性的诱导多潜能干细胞在基因表达谱上很难与胚胎干细胞区分出来，提示人参皂苷Rg1对骨髓间充质干细胞向诱导多潜能干细胞转化可能具有促进作用。

关键词: 人参皂苷Rg1, 骨髓间充质干细胞, 诱导多潜能干细胞, 转化, c-Myc, Nanog

Abstract:

BACKGROUND: Somatic cells are reprogrammed to induced pluripotent stem cells (iPSCs), mainly through anti-retroviral program to transfer Oct-4, Sox2, c-Myc, Klf4 and other genes into somatic cells.
OBJECTIVE: To observe the effects of Ginsenoside Rg1 on Oct4, Sox2, c-Myc, Klf4, Nanog mRNA expression during differentiation of bone marrow mesenchymal stem cells (BMSCs).
METHODS: The BMSCs were cultured; 6.25 μmol/L and 12.5μmol/L Ginsenoside Rg1 of Chinese medicine extraction were added into culture medium, respectively. Oct4, Sox2, c-Myc, Klf4, Nanog mRNA expressions were detected in BMSCs.
RESULTS AND CONCLUSION: In the BMSCs cultured by 6.25 μmol/L Ginsenoside Rg1 for 30 days, the expressions of Nanog, c-Myc, Oct, Klf4, Sox2 mRNA of BMSCs were increased, and the expression of the gene Nanog and c-Myc had significantly difference compared with the control group. It demonstrated that Ginsenoside Rg1 could enhance the expressions of Nanog, c-Myc, which are crucial genes on converting the BMSCs to iPS. But Nanog positive iPS cells are very difficult to be discriminated from the embryonic stem cells in gene expression profile; therefore, we consider that Ginsenoside Rg1 might facilitate the differentiation from BMSCs to iPS.

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