中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (53): 9946-9949.doi: 10.3969/j.issn.1673-8225.2010.53.016

• 移植与免疫 transplantation and Immunology • 上一篇    下一篇

胸腺内注射异基因抗原诱导鼠异体骨移植的免疫耐受

岳伟杰1，崔  勇1，李  卫1，岳  蕾1，谢  刚2，孟庆刚1   

  1. 1 哈尔滨医科大学附属第四医院骨科，黑龙江省哈尔滨市 150001；2 哈尔滨市第四医院骨科，黑龙江省哈尔滨市 150001
  • 出版日期:2010-12-31 发布日期:2010-12-31
  • 通讯作者: 孟庆刚，教授，博士生导师，哈尔滨医科大学附属第四医院骨科，黑龙江省哈尔滨市 150001 mengqinggang2009@126.com
  • 作者简介:岳伟杰★，男，1955年生，黑龙江省哈尔滨市人，汉族，2003年哈尔滨医科大学毕业，硕士，主任医师，教授，主要从事异体骨移植研究。 jbjsbr@126.com
  • 基金资助:

    黑龙江省卫生厅科研课题(2009-178，2009-174)；黑龙江省教育厅资助项目(11541147)；黑龙江省科技计划攻关资助项目(GC10C303-4)。

Intrathymic injection of allogenic antigen induces allograft immune tolerance in rats

Yue Wei-jie1, Cui Yong1, Li Wei1, Yue Lei1, Xie Gang2, Meng Qing-gang1   

  1. 1 Department of Orthopaedics, Fourth Affiliated Hospital of Harbin Medical University, Harbin  150001, Heilongjiang Province, China; 2 Department of Orthopaedics, Harbin Fourth Hospital, Harbin  150001, Heilongjiang Province, China
  • Online:2010-12-31 Published:2010-12-31
  • Contact: Meng Qing-gang, Professor, Doctoral supervisor, Department of Orthopaedics, Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China mengqinggang2009@126.com
  • About author:Yue Wei-jie★, Master, Chief physician, Professor, Department of Orthopaedics, Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China jbjsbr@126.com
  • Supported by:

    Medical Scientific Research of Health Department of Heilongjiang Province, No. 2009-178*, 2009-174*; Supporting Item of Education Department of Heilongjiang Province, No. 11541147*; the Tackle Key Program in Science and Technology of Heilongjiang Province, No.GC10C303-4*

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被引次数

1

摘要：

背景：同种异体骨同其他异体组织一样具有抗原性，移植后可引起以细胞免疫为主的排斥反应，应用免疫抑制剂可抑制免疫排斥反应的发生，但对机体有一定的不良影响。
目的：探讨胸腺内注射异基因抗原在建立特异性骨移植免疫耐受中的作用。
方法：取60只Wistar大鼠随机分为3组，均制作骨缺损模型，供体异基因胸腺内注射组于同种异体骨移植前胸腺内注射受体大鼠脾细胞MHC抗原，同时另设自体骨移植组、同种异体骨移植加用免疫抑制剂组为对照，移植后观察切口情况。移植后1，2，4，6周进行X射线检查，苏木精-伊红染色，可溶性白细胞介素2受体、混合淋巴细胞培养免疫学检测。
结果与结论：大体表现、X射线及组织学检查见供体异基因胸腺内注射组、自体骨移植组表现相近，均有炎性细胞浸润及新骨形成。同种异体骨移植加用免疫抑制剂组再生血管较少，骨愈合延迟，炎性细胞浸润明显，移植骨逐渐吸收，无新骨形成。证实了异基因MHC抗原注入小鼠胸腺内，能成功诱导受体对供体鼠骨移植物的耐受。免疫学检查各项数据表明供体异基因胸腺内注射的成骨效果接近于自体骨移植组，优于同种异体骨移植加用免疫抑制剂，证实了胸腺内注射异体MHC抗原可诱导对供体骨移植物的免疫耐受。

关键词: 胸腺内注射, 异体骨移植, 免疫耐受, 组织细胞移植, 移植

Abstract:

BACKGROUND: Similar to other foreign tissues, allogeneic bones also have antigenicity, which can result in rejection following transplantation. Though the application of immunosuppressant can suppress immunological rejection, it has certain harmful effects to the body.
OBJECTIVE: To investigate the effects of intrathymic injection of allogenic antigen on allograft immune tolerance in rats.
METHODS: Totally 60 Wistar rats were prepared for bone defects models and randomly divided into allogeneic bone graft, autogenous bone graft + allo-antigen group and allogeneic bone graft + immunosuppressant groups. Prior to transplantation, rats in the allogeneic bone graft + allo-antigen group were received intrathymic injections of spleen cells extracted MHC antigens. X-ray examination, hematoxylin-eosin staining were used to observe bone healing at 1, 2, 4 and 6 weeks after transplantation. Meanwhile, the soluble interleukin-2 receptor and mixed lymphocyte were tested by immunological assays.
RESULTS AND CONCLUSION: Gross observation, X-ray film and histological examination showed that there were inflammatory cell infiltration and new bone formation in the allogeneic bone graft + allo-antigen and autogenous bone graft groups. In the allogeneic bone graft + immunosuppressant group, the regenerated vessel was few, and the bone healing was delayed, the inflammatory cell infiltrate was notable, and no new bone was formed. It confirmed that intrathymic injections of spleen cells extracted MHC antigens can induce recipient tolerance to donors bone graft. Immunologic test showed that the bone formation in the allogeneic bone graft + allo-antigen group was similar to the autogenous bone graft group, which was better than the allogeneic bone graft + immunosuppressant group. Thus, intrathymic injection of allo-antigen can induced on the allograft immune tolerance.

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