中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (45): 8390-8393.doi: 10.3969/j.issn.1673-8225.2010.45.008

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

人胎盘间充质干细胞雌、孕激素受体的表达

沙文琼1，王自能2，苏放明1   

  1. 1暨南大学第二临床医学院，深圳市人民医院产科，广东省深圳市  510020；2暨南大学医学院，广东省广州市  510632
  • 出版日期:2010-11-05 发布日期:2010-11-05
  • 作者简介:沙文琼☆，女，1980年生，河北省秦皇岛市人，回族，2010年暨南大学毕业，博士，医师，主要从事胎盘间充质干细胞方面的研究。 swq1980@gmail.com

Expression of estrogen and progestagen receptors in human placental mesenchymal stem cells

Sha Wen-qiong1, Wang Zi-neng2,Su Fang-ming1   

  1. 1 Department of Obstetrics, Shenzhen People’s Hospital, Second Clinical Medical College, Jinan University, Shenzhen  510020, Guangdong Provine, China; 2 Medical College, Jinan University, Guangzhou  510632, Guangdong Province, China
  • Online:2010-11-05 Published:2010-11-05
  • About author:Sha Wen-qiong☆, Doctor, Physician, Department of Obstetrics, Shenzhen People’s Hospital, Second Clinical Medical College, Jinan University, Shenzhen 510020, Guangdong Provine, China swq1980@gmail.com

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421

被引次数

6

摘要：

背景：有研究表明骨髓间充质干细胞受雌、孕激素的调控，并表达雌激素受体。胎盘局部组织雌、孕激素水平较高，可能对胎盘间充质干细胞的生理功能存在影响。
目的：检测人胎盘间充质干细胞的雌、孕激素受体的表达情况。
方法：体外分离和培养人胎盘间充质干细胞，用流式细胞仪检测表面标记物，并做成骨和成脂诱导分化；用反转录荧光定量PCR方法和免疫组织化学方法检测胎盘间充质干细胞的雌、孕激素受体的表达。
结果与结论：人胎盘间充质干细胞阳性表达CD29、CD44、CD73、CD90、CD105和HLA-ABC，阴性表达CD34，CD45，CD14和HLA-DR，具有成骨和成脂诱导分化潜能；PCR结果显示雌激素受体β-mRNA扩增效率最低(△Ct：25.2±0.71)；雌激素受体α-mRNA扩增效率比较低(△Ct：20.64±2.27)；孕激素受体-mRNA扩增效率较好(△Ct：16.11±1.9)。免疫组化结果显示部分细胞孕激素受体抗体染色阳性，阳性率为(18.81±9.16)%；但雌激素受体抗体染色均为阴性。结果提示：人胎盘间充质干细胞部分表达孕激素受体，低水平表达雌激素受体α，基本不表达雌激素受体β。

关键词: 人胎盘间充质干细胞, 雌激素受体, 孕激素受体, 分离培养, 分化潜能

Abstract:

BACKGROUND: It has been shown that estrogen and progestogen regulate bone marrow mesenchymal stem cells by the estrogen and progestagen receptors expressed in them. The high concentrations of estrogen and progestogen in placenta may also have effects on human placental mesenchymal stem cells (MSCs).
OBJECTIVE: To investigate the estrogen and progestagen receptors expression of human placental MSCs.
METHODS: Human placental MSCs were isolated and cultured in vitro. The cell surface markers were determined by flow cytometry. These cells were also induced to osteogenic and adipogenic differentiation. The expression of estrogen and progestagen receptors was detected by fluorescent quantitative polymerase chain reaction and immunohistochemical method.
RESULTS AND CONCLUSION: Human placental MSCs were positive for CD29, CD44, CD73, CD90, CD105 and HLA-ABC, but negative for CD34, CD45, CD14 and HLA-DR. Placental MSCs could be induced to osteoblasts and adipocytes. PCR results showed that the amplification efficiency of estrogen receptor β-mRNA was even lower than it (△Ct: 25.2±0.71), while amplification efficiency of estrogen receptor α-mRNA was low (△Ct: 20.64±2.27). The progestagen receptor had better amplification efficiency  (△Ct: 16.11±1.9). The immunohistochemical results exhibited that positive rate of progestagen receptor was (18.81±9.16)%, but no estrogen receptor was detected in all samples. These data indicate that parts of human placental MSCs expressed progestagen receptor, and human placental MSCs expressed low level estrogen receptor α and no estrogen receptor β.

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