中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (42): 7815-7818.doi: 10.3969/j.issn.1673-8225.2010.42.007

• 组织工程血管材料 tissue-engineered vascular materials • 上一篇    下一篇

雷帕霉素-聚乳酸纳米磁性微球复合材料影响血管平滑肌细胞增殖的能力

吕安林，邢玉洁，王  利，燕学波，赵  伟   

  1. 解放军第四军医大学西京医院心血管内科，陕西省西安市 710032
  • 出版日期:2010-10-15 发布日期:2010-10-15
  • 作者简介:吕安林☆，男，1962年生，山东省济南市人，汉族，1998年解放军第四军医大学毕业，博士，博士后，副教授，主要从事冠状动脉粥样硬化性心脏病的介入治疗、干细胞及心肌组织工程的研究。 lvanlin@yahoo.com.cn

Effect of rapamycin-poly(lactide-acid)/magnetic microsphere composite materials on the proliferation of vascular smooth muscle cells

Lü An-lin, Xing Yu-jie, Wang Li, Yan Xue-bo, Zhao Wei   

  1. Department of Cardiology, Xijing Hospital, Fourth Military Medical University of Chinese PLA, Xi’an   710032, Shaanxi Province, China
  • Online:2010-10-15 Published:2010-10-15
  • About author:Lü An-lin☆, Doctor, Associate professor, Department of Cardiology, Xijing Hospital, Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shaanxi Province, China lvanlin@yahoo.com.cn

PDF

375

被引次数

6

摘要：

背景：聚乳酸类材料是目前常用的血管支架涂层材料，具有可降解、可塑性强、抗原性低和组织相容性好等优点，而磁化的金属裸支架可显著降低血栓和再狭窄的发生。
目的：分析新型支架涂层材料雷帕霉素-聚乳酸纳米磁性微球复合材料的细胞生物相容性及对血管平滑肌增殖的影响。
方法：分散聚合法制作雷帕霉素-聚乳酸纳米磁性微球复合材料小膜片，标准条件下与3~5代SD大鼠主动脉平滑肌细胞共培养7 d，MTT法检测平滑肌细胞的生长增殖情况。
结果与结论：不同磁性微球浓度的聚乳酸复合材料膜片对平滑肌细胞的增殖均有不同程度的抑制作用(P < 0.05)，雷帕霉素-聚乳酸纳米磁性微球复合材料膜片对平滑肌细胞增殖的抑制作用程度与未添加磁性微球的膜片无明显差异(P > 0.05)，但强于未添加雷帕霉素的膜片(P < 0.05)。提示，雷帕霉素-聚乳酸纳米磁性微球复合材料既能显著抑制血管平滑肌细胞增殖又具有很好的细胞生物相容性。

关键词: 雷帕霉素, 纳米, 聚乳酸, 磁性微球, 生物相容性

Abstract:

BACKGROUND: Polylactic acid is a commonly used material for vascular stent coating at present, with advantages of biodegradation, plasticity, low antigenicity and good compatibility. Magnetized metal bare stent may significantly reduce the thrombus and restenosis.
OBJECTIVE: To analyze the cell biocompatibility of a new stent coating composite material, rapamycin-polylactide nano-magnetic microspheres, and investigate the influence on vascular smooth muscle cells proliferation.
METHODS: Small patches of composite material were made of rapamycin-polylactide nano-magnetic microspheres by dispersion polymerization under standard conditions, then were co-cultured with 3-5 passages of rat aortic smooth muscle cells for 7 days, MTT assay was applied to determine the growth and proliferation of smooth muscle cells.
RESULTS AND CONCLUSION: Poly-lactic acid composite membrane of different magnetic microspheres concentrations have different degrees of inhibition on the smooth muscle cell proliferation (P < 0.05). There was no significant difference in the inhibition effect between rapamycin-polylactide nano-magnetic composite microspheres and rapamycin alone (P > 0.05). But the inhibition on the smooth muscle cell proliferation was stronger than single magnetic microspheres of polylactic acid composite membrane (P < 0.05). Composite materials of rapamycin-polylactide nano-magnetic microspheres cam significantly inhibit proliferation of vascular smooth muscle cells, also have good cell biocompatibility.

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