中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (28): 4481-4485.doi: 10.3969/j.issn.2095-4344.2017.28.009

• 口腔组织构建 oral tissue construction • 上一篇    下一篇

小鼠舌菌状乳头及味蕾发育过程中组织形态变化及Shh的表达

李瑞奇1,黄晓峰1,陈光勇2,张 瑞1   

  1. 首都医科大学附属北京友谊医院,1口腔科,2病理科,北京市 100050
  • 修回日期:2017-08-22 出版日期:2017-10-08 发布日期:2017-11-10
  • 通讯作者: 黄晓峰,博士,主任医师,教授,博士生导师,首都医科大学附属北京友谊医院口腔科,北京市 100050
  • 作者简介:李瑞奇,女,1984年生,河北省人,汉族,2014年首都医科大学毕业,硕士,主治医师。
  • 基金资助:

    国家自然科学基金项目(81170943);北京市自然科学基金(7162053)

Histomorphological changes of the fungiform papillae and taste buds as well as the expression of Shh during the tongue development in mice

Li Rui-qi1, Huang Xiao-feng1, Chen Guang-yong2, Zhang Rui1   

  1. 1Department of Stomatology, 2Department of Pathology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
  • Revised:2017-08-22 Online:2017-10-08 Published:2017-11-10
  • Contact: Huang Xiao-feng, M.D., Chief physician, Doctoral supervisor, Department of Stomatology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
  • About author:Li Rui-qi, Master, Attending physician, Department of Stomatology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81170943; the Natural Science Foundation of Beijing, No. 7162053

摘要:

文章快速阅读:

文题释义:
Sonic Hedgehog (Shh):
是Hh信号蛋白家族成员,可通过阶段特异性的自分泌或旁分泌的方式,通过参与上皮间充质之间的相互作用,在菌状乳头和味蕾的形成和维持方面发挥着重要作用。
菌状乳头:主要分布于舌前2/3,每个菌状乳头含有一个或数个味蕾,成熟的味蕾是感知味觉的重要器官。

 

摘要
背景:
哺乳动物的舌背黏膜表面与味觉相关的主要是菌状乳头和轮廓乳头。味觉异常严重影响人们的生活质量,因此了解菌状乳头及其味蕾的发育过程,对于解决因其异常导致的味觉减退有重要意义。
目的:观察小鼠舌菌状乳头及味蕾的发生、发育的组织形态变化。
方法:取C57BL/6J小鼠胚胎期13.5-18.5 d(E13.5-E18.5),出生后0.5 d(PN0.5),PN4.5,PN7.5,PN14.5,PN21.5,PN90.5及出生后1.5年(PN1.5Y)的舌标本,进行扫描电镜观察及苏木精-伊红染色观察,同时使用Shh抗体进行免疫组织化学染色。取E16.5和E18.5小鼠舌标本进行S-100的免疫组织化学染色。
结果与结论:①菌状乳头数量的决定主要发生于E13.5-E14.5。从E15.5开始,菌状乳头形态上逐渐发育成熟。味蕾发育开始于E16.5,至PN7.5味孔开始形成。成年期和老年期味蕾细胞和细胞间质减少,并可见到发生于菌状乳头周围的角化;②Shh在E14.5表达于菌状乳头的上皮细胞,E16.5-E18.5表达于菌状乳头顶端的上皮细胞,并且在成年期和老年期的味蕾细胞都有所表达。说明Shh在菌状乳头和味蕾发生、发育、成熟及维持的过程中发挥着重要作用;③在E18.5,S-100表达于菌状乳头下方的结缔组织,说明神经长入参与了味蕾发育完善成熟的过程。

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
ORCID:
0000-0002-6552-8805(李瑞奇)

关键词: 组织构建, 组织工程, 菌状乳头, 味蕾发育, 胚胎期, 出生后早期, 成年期, 老年期, 小鼠, 扫描电镜, Shh, S-100, 国家自然科学基金

Abstract:

BACKGROUND: Fungiform and circumvallate papillas on the surface of the mammal tongue mucosa are mainly associated with taste. Taste abnormalities seriously affect patients’ quality of life, so it is of significance to understand the developmental processes of fungiform papilla and taste buds, for hypogeusia treatment.
OBJECTIVE: To observe the morphological changes of mouse tongue fungiform papilla and taste buds during their developmental processes.
METHODS: The tongues of C57BL/6J mice were observed by scanning electron microscope and hematoxylin eosin staining at embryonic days 13.5-18.5 (E13.5-E 18.5), postnatal (PN) days 0.5, 4.5, 7.5, 14.5, 21.5, 90.5, and postnatal 1.5 years (PN1.5Y), and Shh antibody was used for immunohistochemical staining. Immunohistochemical staining of S-100 was performed on the tongue specimens of the mice at E16.5 and E18.5.
RESULTS AND CONCLUSION: The number of fungiform papilla was mainly determined at E13.5-E14.5. From E15.5, the papilla gradually matured in morphology. Taste bud development began at E16.5, and the taste pore began to form at PN7.5. In adult and old age, taste bud cells and intercellular substances were reduced, and keratosis surrounding the fungiform papillae was visible. Shh was expressed in the epithelial cells of fungiform papilla at E14.5, in the epithelial cells at the tip of the fungiform papilla at E16.5 and E18.5, and in adult and elderly taste bud cells. These results suggest that Shh plays an important role in the development, maturation and maintenance of fungiform papillae and taste buds. S-100 is expressed in the connective tissue beneath the fungiform papillae at E18.5, indicating that the nerve extension may be involved in the development and maturation of taste buds.

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

Key words: Tongue, Nipples, Taste Buds, Morphogenesis

中图分类号: