中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (25): 4075-4081.doi: 10.3969/j.issn.2095-4344.2017.25.023

• 干细胞综述 stem cell review • 上一篇    下一篇

人脱细胞羊膜在构建组织工程支架中的应用及研究方向

杨继滨,熊华章,李豫皖,刘 毅   

  1. 遵义医学院附属医院骨一科,贵州省遵义市  563000
  • 修回日期:2017-07-03 出版日期:2017-09-08 发布日期:2017-10-09
  • 通讯作者: 刘毅,教授,硕士生导师,遵义医学院附属医院骨一科,贵州省遵义市 563000
  • 作者简介:杨继滨,男,1987年生,贵州省遵义市人,汉族,遵义医学院在读硕士,主要从事干细胞与组织工程,运动医学的研究。
  • 基金资助:

    贵州省科技厅社会发展科技攻关项目(黔社科合SY字[2010]3091);贵州省科学技术基金资助项目(黔科合LH字[2016]7477号)

Application of human acelluar amniotic membrane in tissue engineered scaffold construction

Yang Ji-bin, Xiong Hua-zhang, Li Yu-wan, Liu Yi   

  1. The First Department of Orthopedics, the Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, Chin
  • Revised:2017-07-03 Online:2017-09-08 Published:2017-10-09
  • Contact: Liu Yi, Professor, Master’s supervisor, the First Department of Orthopedics, the Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China
  • About author:Yang Ji-bin, Studying for master’s degree, the First Department of Orthopedics, the Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China
  • Supported by:

    the Science & Technology Program of Guizhou Province, No. SY[2010]3091; the Science & Technology Program of Guizhou Province, No. LH[2016]7477

摘要:

文章快速阅读:

 

文题释义:
脱细胞羊膜制备方法:
包括物理、化学和酶消化法,物理处理通常使用搅动、超声处理、机械按摩、调节压力及反复冻融、以破坏细胞膜并释放细胞内容物;化学处理一般通过使用碱、酸、洗涤剂、有机溶剂、螯合剂、低渗溶液或高渗溶液来破坏细胞膜和负责细胞间和细胞外连接的化学键,达到脱细胞的目的;酶消化法主要使用蛋白酶或核酸酶切割肽键或核苷酸键。

 

摘要
背景:
人脱细胞羊膜是一种良好的具有生物相容性及生物活性的细胞外基质材料,因其免疫原性低、排异反应小、取材容易等优点,被广泛用于临床研究。
目的:综述人脱细胞羊膜在皮肤、血管、角膜、软骨及骨等组织工程中的应用。
方法:由第一作者检索中国知网(2005年1月至2017年5月)、中国生物医学文献数据库(2005年1月至2017年5月)、PubMed数据库(1990年1月至2017年5月)及 Elsevier 数据库(1990年1月至2017年5月),有关人脱细胞羊膜在骨、软骨、皮肤、血管等组织工程中应用的文章,中文关键词为“脱细胞羊膜,支架,组织工程,细胞外基质”,英文关键词为“acelluar amniotic membrane,scaffold,material,tissue engineering,ECM”。
结果与结论:人脱细胞羊膜具有天然的细胞外基质结构及功能,与不同来源干细胞复合后经诱导可向不同的组织和器官分化,例如骨、软骨、皮肤、血管及角膜组织等。人脱细胞羊膜作为组织工程支架具有良好的生物相容性、可降解性及低免疫原性等优点,但其也存在一些不足,包括强度欠佳、植入后可能存在排异反应等。缩短细胞与人脱细胞羊膜支架的黏附时间,增加人脱细胞羊膜的自身机械强度,优化细胞生长微环境,将人脱细胞羊膜与其他组织工程支架相互结合,是今后研究的主要方向。

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程
ORCID: 0000-0003-3074-0053(杨继滨)

关键词: 干细胞, 脱细胞羊膜, 组织工程, 支架, 细胞外基质, 生物相容性, 研究进展, 修复, 重建, 免疫

Abstract:

BACKGROUND: Human acellular amniotic membrane is a kind of extracellular matrix material with good biocompatibility and biological activity. It has been widely used in various clinical studies because of its low immunogenicity, small rejection and easy preparation.
OBJECTIVE: To review the applications of human acellular amniotic membranes in tissue engineering field, such as skin, blood vessel, cornea, cartilage and bone.
METHODS: CNKI (from January 2005 to May 2017), CBMdisc (from January 2005 to May 2017), PubMed (from January 1990 to May 2017) and Elsevier (from January 1990 to May 2017) were retrieved for articles addressing the application of human acellular amniotic membrane as a tissue-engineered scaffold in the bone, cartilage, skin, and blood vessels. The keywords were “acelluar amniotic membrane, scaffold, material, tissue engineering ECM" in Chinese and English, respectively.
RESULTS AND CONCLUSION: Human acellular amniotic membrane owns the structure and function of the natural extracellular matrix, which can be combined with stem cells from different sources to differentiate into different tissues and organs, such as bone, cartilage, skin, blood vessel, and corneal tissues. As a tissue-engineered scaffold, human acellular amniotic membrane has good biocompatibility, biodegradability and low immunogenicity, although it has some shortcomings, such as poor strength and post-transplantation rejection reactions. Therefore, the future studies are mainly focused on shortening the adhesion time between cells and scaffolds, increasing the own mechanical strength of human acellular amniotic membrane, optimizing the cell growth microenvironment, and combining human acellular amniotic membrane with other tissue-engineered scaffolds. 

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Amnion, Excellular Matrix, Tissue Engineering

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