中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (18): 2858-2863.doi: 10.3969/j.issn.2095-4344.2017.18.012

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

三维打印制备不同大孔形态含锶介孔生物玻璃支架的体外细胞实验

张  旭,吴良浩,李得见,敖荣广,陈帆成,俞  斌,禹宝庆   

  1. 复旦大学附属浦东医院骨科,上海市  200120
  • 收稿日期:2017-02-11 出版日期:2017-06-28 发布日期:2017-07-07
  • 通讯作者: 禹宝庆,博士,主任医师,复旦大学附属浦东医院骨科,上海市 200120
  • 作者简介:张旭,男,1988年生,山东省枣庄市人,汉族,复旦大学附属浦东医院骨科在读博士,主要从事三维打印生物可吸收支架实验研究。
  • 基金资助:
    上海市科委基础研究领域项目(13JC1407302);浦东新区卫生系统重点学科群建设资助项目(PWZxq2014-03);上海市卫生局重点课题(20134039)

Three-dimensional printing of strontium-containing mesoporous bioactive glass scaffolds with varied macropore morphologies: an in vitro cytological experiment

Zhang Xu, Wu Liang-hao, Li De-jian, Ao Rong-guang, Chen Fan-cheng, Yu Bin, Yu Bao-qing
  

  1. Department of Orthopedics, Shanghai Pudong Hospital, Fudan University, Shanghai 200120, China
  • Received:2017-02-11 Online:2017-06-28 Published:2017-07-07
  • Contact: Yu Bao-qing, M.D., Chief physician, Department of Orthopedics, Shanghai Pudong Hospital, Fudan University, Shanghai 200120, China
  • About author:Zhang Xu, Studying for doctorate, Department of Orthopedics, Shanghai Pudong Hospital, Fudan University, Shanghai 200120, China
  • Supported by:
     The Shanghai Science and Technology Commission Foundation for Basic Research, No. 13JC1407302; Disciplines Group Construction Project of Pudong Health Bureau of Shanghai, No. PWZxq2014-03; Major Scientific Research Projects of Shanghai Municipal Health Bureau, No. 20134039

摘要:

文章快速阅读:

 

文题释义:
三维打印技术:通过计算机辅助设计(CAD)软件建模,再将建成的三维模型“分区”成逐层的截面,喷头在计算机的控制下,按照截面轮廓的信息,在铺好的一层粉末材料上,有选择性地喷射黏结剂,使部分粉末黏结,形成截面层。一层完成后,工作台下降一个层厚,铺粉,喷黏结剂,再进行后一层的黏结,如此循环形成三维产品。
细胞计数试剂盒8(Cell Counting Kit 8,CCK-8):是一种基于WST-8(2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺酸苯)-2H-四唑单钠盐)广泛应用于细胞增殖和细胞毒性快速高灵敏度检测试剂盒。WST-8在电子载体1-甲氧基-5-甲基吩嗪鎓硫酸二甲酯(1-Methoxy PMS)的作用下可以被线粒体内的脱氢酶还原生成高度水溶性的橙黄色的甲臜产物。生成的甲臜数量与活细胞数量成正比。
 
背景:三维打印支架的大孔孔径形态对生物组织工程支架理化性能及生物性能有很大的影响。
目的:利用三维打印技术制备不同大孔结构的含锶介孔生物玻璃支架,探讨其对MC3T3-E1细胞增殖和成骨分化的影响,寻找最佳的大孔形态。
方法:利用三维打印技术制备含锶介孔生物玻璃/聚己内酯复合支架,其中纵横交错的各纤维状经纬线之间的夹角设置为45°,60°和90°,根据其角度命名为45°,60°,90°含锶介孔生物玻璃支架,检测MC3T3-E1细胞在复合支架上的增殖能力和碱性磷酸酶活性。
结果与结论:①CCK-8方法检测结果表明,3组支架材料都能够支持细胞增殖,第1天和第4天45°含锶介孔生物玻璃支架上的细胞数量略高于60°和90°含锶介孔生物玻璃支架,但差异无显著性意义(P > 0.05);第7天45°含锶介孔生物玻璃支架上的细胞数量显著高于60°和90°含锶介孔生物玻璃支架,差异有显著性意义(P < 0.05);②在第14天,45°含锶介孔生物玻璃支架的细胞碱性磷酸酶活性显著高于60°和90°含锶介孔生物玻璃支架,差异有显著性意意义(P < 0.05);在第21天,3组支架的碱性磷酸酶活性差异无显著性意义(P > 0.05);③结果表明,45°含锶介孔生物玻璃支架相较于60°和90°含锶介孔生物玻璃支架,更能促进MC3T3-E1细胞的增殖和成骨分化。

关键词: 生物材料, 材料相容性, 三维打印, 生物相容性, 含锶介孔生物玻璃, 聚己内酯, MC3T3-E1细胞, 支架, 大孔形态, CCK-8, 碱性磷酸酶

Abstract:

BACKGROUND: Macropore morphology of a composite scaffold prepared by the three-dimensional printing technique is of great importance in determining the physicochemical and biological properties of tissue engineering scaffolds.
OBJECTIVE: To fabricate strontium-containing mesoporous (Sr-MBG) bioactive glass (PCL) scaffolds by the three-dimensional printing technique, and to explore the effect of these scaffolds on MC3T3-E1 proliferation and osteogenic differentiation, thereby to find out the optimal macropore morphology.
METHODS: Sr-MBG/PCL composite scaffolds were fabricated by the three-dimensional printing technique. The angles between fibrous latitudes and longitudes were set to 45°, 60° and 90°. Then the proliferation and alkaline phosphatase activity of MC3T3-E1 cells on the scaffolds were tested.
RESULTS AND CONCLUSION: Cell counting kit-8 results showed that MC3T3-E1 cells could proliferate on all the three kinds of scaffolds. The proliferation rate of MC3T3-E1 cells on the 45° Sr-MBG/PCL scaffolds was just slightly higher than that on the 60° and 90° Sr-MBG/PCL scaffolds at days 1 and 4 (P > 0.05), but there was a significant increase at day 7 (P < 0.05). The 45° Sr-MBG/PCL scaffolds exhibited a significant increase in alkaline phosphatase activity of MC3T3-E1 cells compared to the 60° and 90° Sr-MBG/PCL scaffolds at day 14 (P < 0.05), while there was no significant difference among three groups at day 21 (P > 0.05). These results indicate that the 45° Sr-MBG/PCL scaffold is more suitable to promote the proliferation and osteogenic differentiation of the MC3T3 cells than the 60° and 90° Sr-MBG/PCL scaffolds.

Key words: Biocompatible Materials, Strontium, Osteoblasts, Cell Proliferation, Alkaline Phosphatase, Tissue Engineering

中图分类号: