中国组织工程研究

中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (18): 2839-2845.doi: 10.3969/j.issn.2095-4344.2017.18.009

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

新型多孔钽金属支架材料的生物学评价

谢  辉1，2，马志杰2，王建川2，王本杰2，王  威2，尉晓蔚2，赵德伟1，2
  

  1. 1大连理工大学电信学部生物医学工程系，辽宁省大连市  116023；2大连大学附属中山医院骨科，辽宁省大连市  116001
  • 收稿日期:2017-04-01 出版日期:2017-06-28 发布日期:2017-07-07
  • 通讯作者: 赵德伟，主任医师，教授，大连大学附属中山医院骨一科，辽宁省大连市 116001
  • 作者简介:谢辉，男，安徽省宿州市人，汉族，大连理工大学在读博士，主治医师，主要从事髋关节疾病研究。
  • 基金资助:

    国家十二五科技支撑项目(2012BAI17B02)，项目名称：新一代骨科新型生物医用材料及产品研发；国家十三五“国家重点研发计划” 项目(2016YFC1102000)，项目名称：生物活性脊柱及节段骨缺损修复器械的产品研发

Biological evaluation of a novel porous tantalum scaffold

Xie Hui1, 2, Ma Zhi-jie2, Wang Jian-chuan2, Wang Ben-jie2, Wang Wei2, Wei Xiao-wei2, Zhao De-wei1, 2   

  1. 1Department of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Dalian 116023, Liaoning Province, China; 2Department of Orthopedics, Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China
  • Received:2017-04-01 Online:2017-06-28 Published:2017-07-07
  • Contact: Zhao De-wei, Chief physician, Professor, Department of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Dalian 116023, Liaoning Province, China; Department of Orthopedics, Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China
  • About author:Xie Hui, Studying for doctorate, Attending physician, Department of Biomedical Engineering, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Dalian 116023, Liaoning Province, China; Department of Orthopedics, Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China
  • Supported by:

    the Key Projects in the National Science & Technology Pillar Program during the Twelfth Five-Year Plan Period, No. 2012BAI17B02; the National Major Research and Development Plan during the Thirteenth Five-Year Plan Period, No. 2016YFC1102000

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摘要：

文章快速阅读：

 

文题释义：
多孔钽金属材料：具有高容积孔隙率、低弹性模量和高摩擦系数的特性，受到越来越多的医疗工作者和材料科学家的广泛关注，高容积孔隙率使更多骨组织和富含血管的纤维组织向人工假体内部生长，使植入后的假体与宿主骨产生稳定连接；低弹性模量使假体植入后产生较少的应力遮挡效应，有利于正常的生物学应力传导；高摩擦系数使假体植入后能保持较好的初始稳定性，因此其特别适用于骨替代、关节置换和人体组织填充等领域。
化学气相沉积技术：是传统的制备薄膜的技术，其原理是利用气态的先驱反应物，通过原子、分子间化学反应，使得气态前驱体中的某些成分分解，而在基体上形成薄膜。化学气相沉积包括常压化学气相沉积、等离子体辅助化学沉积、激光辅助化学沉积、金属有机化合物沉积等。

背景：利用涂层制备技术在传统材料基体上沉积金属钽涂层，既利用了金属钽优异生物学性能又可降低成本，为金属钽器件的应用提供了一条切实可行的方向。
目的：制备金属钽涂层支架材料，观察其体内外生物相容性。
方法：采用常压化学气相沉积技术在多孔碳化硅基体上制备多孔金属钽涂层支架材料，进行以下实验：①体外实验：将骨髓间充质干细胞与多孔金属钽涂层支架材料共培养2周，MTT法检测细胞增殖；培养第5，10，15天，扫描电镜观察细胞在材料表面附着情况；②体内实验：在犬股骨头骨缺损处植入多孔钽材料，分别于植入后6，12周处死实验动物，将标本硬组织切片染色后，显微镜下观察多孔钽材料与周围组织的生长情况。
结果与结论：①体外实验结果：随着培养时间的延长，骨髓间充质干细胞呈现出极其旺盛的增殖，相互排列紧密，细胞之间连接并爬行生长进入多孔钽孔隙中，细胞完全覆盖在多孔钽表面，细胞与细胞之间形成团状重叠现象；②体内实验结果：植入6周，材料与骨组织周围界限清晰，多孔钽孔隙中有少量骨组织爬行，有少许骨小梁长入，未附着部分可见孔隙周围空虚及缝隙，材料周围未见组织破坏及排斥反应；植入12周，可明显观察到材料与骨组织生长良好，多孔钽表面和孔隙内大量骨组织长入，材料孔隙与组织紧密连接，有大量骨小梁长入，多孔钽与骨组织融于一体；③结果表明：采用常压化学气相沉积技术制备的金属钽涂层支架材料具有良好的生物相容性。

关键词: 生物材料, 材料相容性, 碳化硅, 多孔钽, 化学气相沉积法, 骨髓间充质干细胞, 生物相容性

Abstract:

BACKGROUND: Based on the excellent biological performance of tantalum, tantalum coating can be prepared on the conventional substrate by the coating preparation technique, which lowers the cost and importantly provides a feasible direction for the application of tantalum metal devices.
OBJECTIVE: To study the biocompatibility of porous silicon carbide scaffold with tantalum coating.
METHODS: By atmospheric pressure chemical vapor deposition, the porous tantalum-coated silicon carbide scaffold was prepared. In an in vitro experiment, bone marrow mesenchymal stem cells were co-cultured with the porous tantalum-coated silicon carbide scaffold for 2 weeks. Then cell proliferation was detected using MTT method. Cell adhesion on the scaffold was observed using scanning electron microscope at 5, 10, 15 days after culture. In an in vivo experiment, the porous tantalum-coated silicon carbide material was implanted into the region of femoral head defects in dogs. Experimental animals were killed at 6, 12 weeks after implantation, and then the specimens of hard tissue sections were stained to observe the implant material and growth of its surrounding tissues under the microscope.
RESULTS AND CONCLUSION: (1) In vitro experiment results: with increasing time in the co-culture, bone marrow mesenchymal stem cells exhibited very strong proliferation, mutual connection and close arrangement. Interconnected cells crept into the pores of the porous tantalum-coated silicon carbide scaffold, and completely covered the tantalum surface, then forming overlapped cell clusters. (2) In vivo experimental results: 6 weeks after implantation, there was a clear boundary between the implant and surrounding bone tissues, and only a small amount of bone tissues crept into the porous tantalum-coated silicon carbide material in the presence of a little trabecular bone. Some voids and cracks around the pores of the implant were detectable. Neither damage nor rejection of tissues around materials occurred. At 12 weeks after implantation, the implant was fused well with the surrounding tissues in the presence of a large amount of bone tissues growing into the surface and pores and a large number of trabecular bones. To conclude, the tantalum-coated silicon carbide scaffold prepared by the atmospheric pressure chemical vapor deposition has good biocompatibility.

Key words: Materials Testing, Tantalum, Stem Cells, Tissue Engineering

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