中国组织工程研究

中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (18): 2846-2851.doi: 10.3969/j.issn.2095-4344.2017.18.010

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

镁合金材料与人内皮细胞的生物相容性

王心玲1，胡桐楠2，储冰峰2
  

  1. 1解放军总后勤部第一门诊部口腔中心，北京市  100842；2解放军总医院口腔科，北京市  100853
  • 收稿日期:2017-01-13 出版日期:2017-06-28 发布日期:2017-07-07
  • 通讯作者: 胡桐楠，在读硕士，解放军总医院口腔科，北京市 100853
  • 作者简介:王心玲，女，1980年生，山东省高密市人，汉族，2004年解放军第二军医大学长海医院毕业，硕士，副主任医师，主要从事口腔修复研究。
  • 基金资助:

    军队医学科技青年培育项目(130NP198)：不同致龋性变形链球菌差异非编码小RNA的筛选与功能研究

Biocompatibility of magnesium alloys with human endothelial cells

Wang Xin-ling1, Hu Tong-nan2, Chu Bing-feng2
  

  1. 1Stomatological Center, First Out-patient Department of PLA General Logistics Department, Beijing 100842, China; 2Department of Stomatology, PLA General Hospital, Beijing 100853, China
  • Received:2017-01-13 Online:2017-06-28 Published:2017-07-07
  • Contact: Hu Tong-nan, Studying for master’s degree, Department of Stomatology, PLA General Hospital, Beijing 100853, China
  • About author:Wang Xin-ling, Master, Associate chief physician, Stomatological Center, First Out-patient Department of PLA General Logistics Department, Beijing 100842, China
  • Supported by:

    the Military Medical Science and Technology Training Project for the Youth, No. 130NP198

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摘要：

文章快速阅读：

 

文题释义：
镁及镁合金：比刚度和比强度高，密度小，弹性模量与人骨密度相接近，具有良好的生物相容性，同时其具有生物可降解性，容易被机体吸收，属于常量元素。镁合金在生物体降解过程中释放的镁离子又能参与机体的生理代谢，发挥补镁的生理作用，在口腔颌面部颌骨生物医用材料领域具有广阔的应用前景。
镁合金的细胞相容性：研究釆用直接接触法将人脐静脉内皮细胞接种到镁合金表面，研究镁合金对人脐静脉内皮细胞生长形态的影响，进而探讨镁合金血管支架在口腔领域的应用前景。制备镁合金浸提液配置为条件培养基，诱导培养细胞，采用间接法研究合金对血管内皮细胞的影响。

背景：镁合金是一种易降解且具有良好力学性能的材料。
目的：研究AM50和WE43铸造镁合金对人脐静脉血管内皮细胞增殖及生长形态的影响。
方法：取对数生长期的人脐静脉血管内皮细胞，直接接种于AM50铸造镁合金或WE43铸造镁合金表面，培养24 h后，免疫荧光染色观察细胞形态。取对数生长期的人脐静脉血管内皮细胞，分别以100%、50%、10%浓度的AM50铸造镁合金或WE43铸造镁合金浸提液培养，以常规培养的细胞为阴性对照组，培养12，24，48 h后，检测细胞相对增殖率；培养24 h后，免疫荧光染色观察细胞形态，同时检测细胞上清液pH值与元素水平。
结果与结论：①细胞相对增殖率：与对照组相比，不同浓度的AM50和WE43镁合金浸提液对人脐静脉内皮细胞增殖无影响；②免疫荧光染色观察结果：接种于AM50和WE43镁合金表面的人脐静脉血管内皮细胞面积较阴性对照组缩小，伸展不足，胞浆内细胞骨架结构模糊；分别以10%、50%、100% AM50铸造镁合金浸提液或WE43铸造镁合金浸提液处理人脐静脉血管内皮细胞 24 h，细胞形态与阴性对照组细胞较一致，胞浆内细胞骨架结构清楚；③细胞上清液pH值与元素含量：与阴性对照组相比，100%浓度AM50和WE43镁合金浸提液组细胞上清pH明显上升(P < 0.001)，镁元素质量浓度明显升高；④结果表明：AM50和WE43铸造镁合金对人脐静脉血管内皮细胞增殖及生长无明显影响，具有良好的细胞相容性。

关键词: 生物材料, 材料相容性, 镁合金, 内皮细胞, 细胞增殖, 生物相容性, 口腔生物材料

Abstract:

BACKGROUND: Magnesium alloy is a material that is easily degradable and has good mechanical properties.
OBJECTIVE: To study the effects of the two kinds of magnesium alloy materials (AM50 and WE43) on proliferation and morphology of human umbilical vein endothelial cells.
METHODS: Human umbilical vein endothelial cells at logarithmic phase were cultured in 100%, 50%, 10% extracts of AM50 and WE43 or directly seeded on the surface of AM50 and WE43 for 24 hours to study the morphological effect of magnesium alloys on the cells. Human umbilical vein endothelial cells cultured routinely served as negative controls. Relative growth rate of the cells was detected at 12, 24, 48 hours after culture. Cell morphology was observed using immunofluorescence staining at 24 hours after culture, and pH and element levels in the cell supernatant were measured.
RESULTS AND CONCLUSION: (1) AM50 and WE43 extracts showed no effects on the proliferation of human umbilical vein endothelial cells in comparison with the negative control group. (2) Compared with the negative control group, reduced area of human umbilical vein endothelial cells was found on the surface of AM50 or WE43 alloy by the immunofluorescent staining, and the cytoskeleton of the cytoplasm was blurred. However, after 24 hours of culture, morphological changes of the cells cultured in 10%, 50% and 100% AM50 or WE43 extracts were consistent with those cultured routinely. The cytoskeleton of the cytoplasm was clearly visible. (3) Compared with the negative control group, the pH values of 100% AM50 and WE43 extract groups were significantly upregulated, and the level of magnesium element was also increased remarkably. In summary, AM50 and WE43 alloys possess good cytocompatibility with human umbilical vein endothelial cells, and have no influence on the cell proliferation and growth.   

Key words: Dental Alloys, Cell Proliferation, Endothelial Cells, Tissue Engineering

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