中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (10): 1514-1519.doi: 10.3969/j.issn.2095-4344.2017.10.007

• 药物控释材料 drug delivery materials • 上一篇    下一篇

人β-防御素3/聚乳酸-羟基乙酸缓释微球制备及体外释药性能

孙治邦,周义钦,陈  松,吴海山   

  1. 解放军第二军医大学附属长征医院,上海市  200003
  • 收稿日期:2017-02-13 出版日期:2017-04-08 发布日期:2017-05-08
  • 通讯作者: 吴海山,博士,教授,解放军第二军医大学附属长征医院,上海市 200003
  • 作者简介:孙治邦,男,1988年生,安徽省六安市人,汉族,解放军第二军医大学在读硕士,主要从事关节置换感染方面的研究。
  • 基金资助:

    上海市卫生局课题(2012353),课题名称:β-防御素-3/聚乳酸聚乙醇酸缓释微球抑制人工关节术后生物膜形成的机制研究

Human beta defense 3/poly(lactic-co-glycolic acid) controlled-release microspheres: preparation and in vitro releasing performance

Sun Zhi-bang, Zhou Yi-qin, Chen Song, Wu Hai-shan   

  1. Changzheng Hospital, Second Military Medical University of PLA, Shanghai 200003, China
  • Received:2017-02-13 Online:2017-04-08 Published:2017-05-08
  • Contact: Wu Hai-shan, M.D., Professor, Changzheng Hospital, Second Military Medical University of PLA, Shanghai 200003, China
  • About author:Sun Zhi-bang, Studying for master’s degree, Changzheng Hospital, Second Military Medical University of PLA, Shanghai 200003, China
  • Supported by:

    the Project of Shanghai Health Bureau, No. 2012353

摘要:

文章快速阅读:


 

文题释义:
人防御素-3
:是首先在银屑病患者的皮损组织中被发现的一种内源性小分子多肽,后续的研究表明它在人体的上皮细胞和黏膜组织中均存在广泛的表达。人防御素-3具有广谱的抗菌性能,同时也有报道具有促进愈合、抗肿瘤等多种特性。
体外释药性能:在体外对药物进行释放速率的监测,以观察药物的释放特性,判断药物的作用时间以及为后续用量提供参考。


背景:目前,关节置换后感染的治疗方法为早期的一期清创手术和标准的二期翻修手术,单纯使用抗生素治疗疗效甚微,课题组拟使用载药缓释微球来探索局部抑制感染治疗方法的可行性。
目的:观察人β-防御素3/聚乳酸-羟基乙酸缓释微球的理化性质及体外释药特性。
方法:采用改良复乳-溶剂挥发法,以聚乳酸-羟基乙酸为载体材料,人β-防御素3为药物制备人β-防御素3/聚乳酸-羟基乙酸缓释微球。采用扫描电镜观察其表面形态,标尺精确测量每个微球大小,采用分光光度计测定微球的载药量和包封率,通过测定人β-防御素3/聚乳酸-羟基乙酸微球的缓释时间,分析微球的体外释药特性。
结果与结论:①人β-防御素3/聚乳酸-羟基乙酸缓释微球的表面光滑,均匀分布,流动性良好,其平均粒径为219.49 nm;②人β-防御素3的载药量为(20.67±0.17)%,包封率为(54.52±1.31)%;③人β-防御素3的25 d累计释药量平均为(74.12±0.43)%;④结果表明,人β-防御素3/聚乳酸-羟基乙酸缓释微球的体外释药性能良好,理论上可以达到缓释抗菌的目的,为后续的动物抗菌实验研究奠定基础。

ORCID: 0000-0002-4479-7002(孙怡邦)

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程

关键词: 生物材料, 缓释材料, HBD-3/PLGA缓释微球, 理化性质, 载药量, 包封率, 体外释药性能

Abstract:

BACKGROUND: A simple use of antibiotic drugs as anti-infection therapy after joint replacement is not enough for subsequent debridement and secondary revision surgeries. Therefore, our team intended to confirm the feasible use of controlled-release microspheres in the local anti-infection treatment.
OBJECTIVE: To prepare the Human beta defense 3 (HBD-3)/poly(lactic-co-glycolic acid) (PLGA) micro-spheres and to investigate the microsphere physicochemical properties and drug release characteristics in vitro.
METHODS: With PLGA as a carrier, HBD-3/PLGA controlled-release microspheres were prepared by using double emulsion-solvent evaporation method. Scanning electron microscopy was used to observe its surface morphology. The size of each microsphere was accurately determined using scaleplate. Drug loading capacity and encapsulation efficiency of HBD-3/PLGA controlled-release microspheres were calculated using spectrophotometer. HBD-3/PLGA microsphere controlled-release time was determined in order to analyze the drug release characteristics of the microsphere.
RESULTS AND CONCLUSION: The HBD-3/PLGA controlled-release microsphere possessed smooth surface, uniform distribution and good liquidity. The average particle size was 219.49 nm, the drug loading capacity of HBD-3 was (20.67±0.17)% and the encapsulation efficiency was (54.52±1.31)%. The cumulative release percentage of HBD-3 was (74.12±0.43)%. The HBD-3/PLGA controlled-release microsphere has well controlled-release performance in vitro. In theory, the purpose of antibacterial controlled-release can be achieved, laying a foundation for subsequent animal antibacterial experiments.

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程

Key words: beta-Defensins, Delayed-Action Preparations, Arthroplasty, Replacement, Infection, Tissue Engineering

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