中国组织工程研究

中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (6): 859-863.doi: 10.3969/j.issn.2095-4344.2017.06.007

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犬股动脉置换壳聚糖-硫酸化丝素蛋白人工血管：内皮层及血管层的生成

马  力1，谢宜旭1，常  羽1，马  立2
  

  1. 1郑州大学附属郑州市中心医院心胸外科，河南省郑州市  450007；2河南省人民医院呼吸科，河南省郑州市  450002
  • 收稿日期:2017-01-11 出版日期:2017-02-28 发布日期:2017-03-16
  • 作者简介:马力，男，1973年生，河南省开封市人，副主任医师，主要从事新心胸外科方面研究。
  • 基金资助:

    河南省科学技术厅项目(152300410148)

Canine femoral artery displacement with the chitosan-sulfated silk fibroin artificial blood vessel: the formation of vascular endothelial cells, vascular smooth muscle cells and fibroblasts

Ma Li1, Xie Yi-xu1, Chang Yu1, Ma Li2
  

  1. 1Department of Cardiothoracic Surgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, Henan Province, China; 2Department of Respiratory Medicine, Henan Provincial People’s Hospital, Zhengzhou 450002, Henan Province, China
  • Received:2017-01-11 Online:2017-02-28 Published:2017-03-16
  • About author:Ma Li, Associate chief physician, Department of Cardiothoracic Surgery, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007, Henan Province, China
  • Supported by:

    the Project of the Science and Technology Department of Henan Province, No. 152300410148

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摘要：

文章快速阅读：

 

文题释义：
组织工程血管：组织工程是将工程学和生命科学进行结合，利用生物材料制备病损组织模型，并经过在模型上种植细胞，达到模仿病损组织的形态，使其恢复为病损前的结构并具备该组织原有功能的目的。通过体外培养种子细胞，选择适合种子细胞生长的可降解“支架”材料，将支架与细胞共生体植入患病部位。当“支架”降解后，细胞逐渐在病损部位增殖进而恢复病损组织的正常结构和应有功能，进而对创伤进行修复并最终恢复该组织原有功能。
生物相容性：分为生物学反应和材料反应两部分，其中生物反应包括血液反应，免疫反应和组织反应；材料反应主要表现在材料物理和化学性质的改变。生物相容性主要决定于材料的性质和用途。材料及制品本身的性质，包括形状、大小及表面粗糙程度，材料聚合或制备过程残留的有毒低分子物质、材料加工工艺污染、材料在体内的降解产物等都与其生物相容性相关。当考虑将材料用于生物医学领域时，其生物相容性是需要考虑和评价的重要指标。

背景：前期实验制备了壳聚糖-硫酸化丝素蛋白人工血管。
目的：通过犬股动脉置换壳聚糖-硫酸化丝素蛋白人工血管，探讨组织工程血管的功效性能。
方法：将8只比格犬随机分为2组，实验组于股动脉进行壳聚糖-硫酸化丝素蛋白人工血管置换；正常对照组不进行任何处理。置换后6个月，检测凝血四项后，采用苏木精-伊红染色观察血管结构，免疫组织化学观察血管结构形成，电镜观察血管内皮层及血管层生成情况。
结果与结论：①苏木精-伊红染色：实验组血管形成了内皮细胞层、平滑肌层和成纤维细胞层3层主要血管组织，在组织形态上与正常对照组血管无显著差别；②免疫组织化学染色：实验组FactorⅧ染色可见组织边缘一深色带染色即为内皮层形成；á-SMA染色可见较多棕色颗粒沉淀，为血管平滑肌形成；Vimentin染色亦可见较多棕色颗粒沉淀，提示人工血管也形成了成纤维细胞层。该3层组织与正常对照组血管无明显差异；③电镜观察：实验组人工血管内表面及切面与正常对照组血管相似，切面也形成了3层组织，但排列不及正常血管整齐；④凝血四项：两组凝血酶原时间、活化部分凝血活酶时间、凝血酶时间、纤维蛋白原无差异；⑤结果表明：壳聚糖-硫酸化丝素蛋白人工血管具有较好的生物相容性及功效性能。

关键词: 生物材料, 材料相容性, 人工血管, 丝素蛋白, 壳聚糖, 凝血四项

Abstract:

BACKGROUND: The chitosan-sulfated silk fibroin artificial blood vessel was obtained in our previous study.
OBJECTIVE: To investigate the biocompatibility and function of the chitosan-sulfated silk fibroin artificial blood vessel replacement into the canine femoral artery.
METHODS: Eight Beagles were randomly allotted to two groups, and chitosan-sulfated silk fibroin artificial blood vessel was implanted into the femoral artery (experimental group), but animalsin control group received no intervention. Six months after implantation, the coagulation function was detected, the vascular formation, including endothelial, smooth muscle and fibroblast layers were observed through hematoxylin-eosin staining under electron microscope.
RESULTS AND CONCLUSION: (1) Hematoxylin-eosin staining showed that vascular endothelial cells, vascular smooth muscle cells and fibroblasts were found in the experimental group, which were similar with the normal vascular structures. (2) Immunohistochemistry: in the experimental group, there was a deeply stained band at the tissue edege through Factor VIII staining, suggesting the formation of the endothelium; there were abundant brown particles precipitated in á-SMA staining, suggesting the vascular smooth muscle cell formation; Vimentin staining showed various brown particle precipitations indicating the fibroblast formation, and all were close to the normal vascular structures. (3) Electron microscope observed that the inner surface and section of the artificial vessel were similar with the normal one, and the three-layer structures formed in the section arranged irregularly. (4) There were no differences in the prothrombin time, activated partial thromboplastin time, thrombin time and fibrinogen between groups. To conclude, these results suggest that the chitosan-sulfated silk fibroin artificial blood vessel holds good biocompatibility and performances.

Key words: Blood Vessel Prosthesis, Silk, Chitosan, Tissue Engineering

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