中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (49): 8005-8009.doi: 10.3969/j.issn.2095-4344.2015.49.023

• 有限元分析动物模型 Finite element analysis of animal models • 上一篇    下一篇

肛门直肠有限元模型黏膜节点位移量评价消痔灵注射的效果

柯敏辉1，叶 玲1，陈立武1，牛素生2，郑鸣霄1，张之玺3   

  1. 1福建中医药大学附属第二人民医院肛肠科，福建省福州市 350003；2福建中医药大学骨伤学院，福建省福州市 350122；3福州大学光弹实验室，福建省福州市 350108
  • 收稿日期:2015-09-06 出版日期:2015-11-30 发布日期:2015-11-30
  • 通讯作者: 叶玲，主任医师，福建中医药大学附属第二人民医院肛肠科，福建省福州市 350003
  • 作者简介:柯敏辉，男，1981年生，福建省福州市人，2005年福建中医药大学毕业，硕士，主治医师，主要从事肛门直肠疾病的临床研究。
  • 基金资助:

    国家自然科学基金面上项目(81173272)；福建省自然基金项目(2015J01402)

Evaluation of Xiaozhiling injection effect on rectal internal mucosal prolapse based on the amount of displacement of mucosal nodes in a finite element model of rabbit anorectum

Ke Min-hui1, Ye Ling1, Chen Li-wu1, Niu Su-sheng2, Zheng Ming-xiao1, Zhang Zhi-xi3   

  1. 1Department of Anorectal Surgery, Second People’s Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China; 2College of Osteopathy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China; 3Laboratory of Photoelasticity, Fuzhou University, Fuzhou 350108, Fujian Province, China
  • Received:2015-09-06 Online:2015-11-30 Published:2015-11-30
  • Contact: Ye Ling, Chief physician, Department of Anorectal Surgery, Second People’s Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China
  • About author:Ke Min-hui, Master, Attending physician, Department of Anorectal Surgery, Second People’s Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81173272; the Natural Foundation of Fujian Province of China, No. 2015J01402

PDF

307

被引次数

5

摘要：

背景：前期研究确立了与人直肠黏膜内脱垂相似、稳定性好的2个月直肠黏膜内脱垂兔造模方案。目前中医治疗直肠黏膜内脱垂以消痔灵注射为主，其近期疗效肯定，但远期仍存在着一定的复发率。
目的：借助兔肛门直肠有限元模型黏膜节点的位移量评价消痔灵注射治疗直肠黏膜内脱垂大效果。
方法：将32只兔按直肠黏膜内脱垂造模方案造模，造模后随机分为2组：一组为直肠黏膜内脱垂组8只兔，分组后即处死解剖取其直肠进行力学指标测算；一组为消痔灵注射组24只兔，于注射后第3，7，14天分别随机选取8只兔解剖出直肠进行相同的力学指标测算。最后将2组数据输入兔肛门直肠有限元模型，在肛门直肠角上方、肛门直肠角平面、肛门直肠角下方3个横断面的直肠黏膜上，各选取不同冠状位(腹侧、中间位、背侧)的节点，在模拟排便受力的情况下测算这些节点的位移量。
结果与结论：经各节点内部比较，在消痔灵注射前后不同时期，每个节点的位移量均存在显著性差异；经节点间比较，在消痔灵注射前后的相同时期，同一横断面节点间的位移量存在显著性差异，同一冠状面节点间的位移量同样存在显著性差异。提示消痔灵注射治疗直肠黏膜内脱垂有效，但注射后直肠黏膜有限元不同节点位移量的差异可能是影响疗效的因素之一。 

关键词: 实验动物模型, 有限元分析动物模型, 组织构建, 直肠黏膜内脱垂, 消痔灵, 有限元, 节点, 国家自然科学基金

Abstract:

BACKGROUND: Previous studies have confirmed a scheme of establishing rabbit models of rectal internal mucosal prolapse within 2 months, which were similar to rectal internal mucosal prolapse in humans and had good stability. At present, Xiaozhiling injection is a preferred traditional Chinese medicine for treatment of rectal internal mucosal prolapse. Its short-term curative effect is positive, but rectal internal mucosal prolapse recurs after long-term administration of Xiaozhiling injection.
OBJECTIVE: To evaluate the effect of Xiaozhiling injection in the treatment of rectal internal mucosal prolapse based on the amount of displacement of mucosal nodes in a finite element model of rabbit anorectum.
METHODS: Thirty-two rabbit models of rectal internal mucosal prolapse were randomly divided into rectal internal mucosal prolapse group (n=8) and Xiaozhiling injection group (n=24). Rbbits in the rectal internal mucosal prolapse group were sacrificed and dissected to harvest the rectum for mechanical measurements. At the 3rd, 7th and 14th days after administration of Xiaozhiling injection, eight rabbits per time point from the Xiaozhiling 
injection group were sacrificed to harvest the rectum for mechanical measurements. At last, the data from these two groups were input into a finite element model of the rabbit anorectum. The nodes in different coronal planes (ventral, middle and dorsal) were selected on three rectal mucosal cross-sections harvested from different areas (above, at and below the anorectal angle). The amounts of displacement of these nodes were measured under the condition of simulated defecation.
RESULTS AND CONCLUSION: There was significant difference in the amount of displacement of each node between different time periods before and after administration of Xiaozhiling injection. There was significant difference in the amount of displacement between mucosal nodes from the same cross-section and between mucosal nodes from the same coronal plane in the same time period before and after administration of Xiaozhiling injection. These results suggest that Xiaozhiling injection is effective in the treatment of rectal internal mucosal prolapse, however, the difference in amount of displacement of different nodes in the rectal mucosa finite element after administration of Xiaozhiling injection may be one of the factors affecting the efficacy.  

中图分类号: