中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (45): 7309-7313.doi: 10.3969/j.issn.2095-4344.2015.45.017

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

针刺联合神经干细胞移植对脑性瘫痪幼鼠模型的作用机制

介小素，侯玉晋   

  1. 河南中医学院第二附属医院儿童康复科，河南省郑州市 450002
  • 收稿日期:2015-09-20 出版日期:2015-11-05 发布日期:2015-11-05
  • 通讯作者: 介小素，河南中医学院第二附属医院儿童康复科，河南省郑州市 450002
  • 作者简介:介小素，女，1977年生，河南省人，汉族，2007年广西中医学院毕业，硕士，主治医师。
  • 基金资助:

     河南中医学院苗圃工程项目(MP2011-16)

Combined therapy of acupuncture and neural stem cell transplantation in immature rats with cerebral palsy

Jie Xiao-su, Hou Yu-jin   

  1. Department of Children Rehabilitation, Second Affiliated Hospital, Henan University of TCM, Zhengzhou 450002, Henan Province, China
  • Received:2015-09-20 Online:2015-11-05 Published:2015-11-05
  • Contact: Jie Xiao-su, Department of Children Rehabilitation, Second Affiliated Hospital, Henan University of TCM, Zhengzhou 450002, Henan Province, China
  • About author:Jie Xiao-su, Master, Attending physician, Department of Children Rehabilitation, Second Affiliated Hospital, Henan University of TCM, Zhengzhou 450002, Henan Province, China
  • Supported by:

    the Nursery Garden Engineering of Henan University of TCM, No. MP2011-16

PDF

382

被引次数

5

摘要：

背景：针灸治疗脑性瘫痪效果显著，神经干细胞治疗也可以改善脑性瘫痪症状，对于针灸和神经干细胞联合治疗脑性瘫痪的研究较少。
目的：观察针刺和神经干细胞联合治疗对脑性瘫痪鼠的影响及其作用机制。
方法：建立幼鼠脑性瘫痪模型，分别给与单纯针刺、单纯神经干细胞移植、针刺和神经干细胞联合治疗，同时设正常对照组和模型组作对比。
结果与结论：与模型组、单纯针刺组、单纯神经干细胞移植组相比，针刺和神经干细胞联合治疗组的脑性瘫痪幼鼠行为学评分明显改善(P < 0.05)，神经细胞数量和Bcl-2蛋白表达增高(P < 0.05)，凋亡神经细胞数量和Bax蛋白表达降低(P < 0.05)。模型组、单纯针刺组、单纯神经干细胞移植组上述各指标变化差异不显著。结果说明，针刺+神经干细胞联合治疗可以明显改善脑性瘫痪幼鼠的运动学习和记忆能力，减轻神经细胞凋亡，其机制可能与其抑制Bax蛋白表达，上调Bcl-2蛋白表达有关。
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 干细胞, 移植, 神经干细胞, 针刺, 治疗, 脑性瘫痪, 大鼠, Bax, Bcl-2, 凋亡

Abstract:

BACKGROUND: Both acupuncture and neural stem cell therapy have good achievements in the treatment of cerebral palsy, but there are few reports about their combined therapy.
OBJECTIVE: To observe the therapeutic effect of combined therapy of acupuncture and neural stem cell transplantation on cerebral palsy rats and to explore the relevant mechanism of action.
METHODS: Cerebral palsy models were built in newborn rats, and then, the model rats were subjected to acupuncture, neural stem cell transplantation and their combination, respectively. Meanwhile, normal control group and model group were set up.
RESULTS AND CONCLUSION: Compared with the other groups, in the combined therapy group, the behavior scores of cerebral palsy rats improved significantly (P < 0.05), the number of nerve cells and Bcl-2 protein expression were increased significantly (P < 0.05), and the number of apoptotic nerve cells and Bax protein expression were decreased significantly (P < 0.05). Additionally, there were no significant differences in these indicators among the model, acupuncture and neural stem cell transplantation groups. These findings indicate that the combined therapy of acupuncture and neural stem cell therapy can obviously improve learning and memory ability of cerebral palsy rats and reduce neuronal apoptosis, which is probably associated with down-regulation of Bax protein and up-regulation of Bcl-2 protein. 
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Tissue Engineering, Stem Cells, Acupuncture, Apoptosis