中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (49): 7985-7989.doi: 10.3969/j.issn.2095-4344.2014.49.020

• 器官移植动物模型 organ transplantation and animal model • 上一篇    下一篇

科研型肝癌肝移植临床标本库的建立及意义

张 庆1,郝昱文2,岳 扬1,陈 虹1,王乐天1,陈新国1,沈中阳1   

  1. 武警总医院,1肝移植研究所,2计算机管理中心,北京市 100039
  • 修回日期:2014-09-03 出版日期:2014-11-30 发布日期:2014-11-30
  • 通讯作者: 沈中阳,主任医师,博士生导师,武警总医院器官移植研究所,北京市 100039
  • 作者简介:张庆,女,1969年生,汉族,博士,副主任医师,硕士生导师,主要从事肝癌的发病及其防治研究。
  • 基金资助:

    国家自然科学基金项目(81372595);首都临床特色应用研究项目(Z121107001012168);武警部队资助科研项目二类(WZ 2010027)

Establishment and significance of research-based clinical liver transplantation specimen bank for hepatocellular carcinoma  

Zhang Qing1, Hao Yu-wen2, Yue Yang1, Chen Hong1, Wang Le-tian1, Chen Xin-guo1, Shen Zhong-yang1   

  1. 1Institute of Liver Transplantation, 2Computer Management Center, General Hospital of Chinese People’s Armed Police Force, Beijing 100039, China
  • Revised:2014-09-03 Online:2014-11-30 Published:2014-11-30
  • Contact: Shen Zhong-yang, Chief physician, Doctoral supervisor, Institute of Liver Transplantation, General Hospital of Chinese People’s Armed Police Force, Beijing 100039, China
  • About author:Zhang Qing, M.D., Associate chief physician, Master’s supervisor, Institute of Liver Transplantation, General Hospital of Chinese People’s Armed Police Force, Beijing 100039, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81372595; the Capital Clinical Characteristics Applied Research Project, No. Z121107001012168; the Armed Forces Scientific Research Project, No. WZ 2010027

     

摘要:

背景:建立规范化的肝癌肝移植临床样本库可为开展相关领域研究提供资源平台,是进行科学研究的先决条件。

目的:初步建立科研型肝癌肝移植临床标本库,研究并制定规范化的肝癌肝移植标本采集、处理、保存操作流程,建立完善、全面的肝癌肝癌肝移植标本信息化管理系统。
方法:鉴国内外标本库建立的标准化程序,制定肝癌肝移植标本库操作流程及质量控制体系,规范化收集、处理及保存肝癌肝移植受者的肿瘤组织及血液标本,同时收集良性肝病肝移植受者及健康供者的肝组织及血液标本作为研究对照。对所收集的标本及相关临床资料信息进行系统化管理。
结果与结论:自2009年8月肝癌肝移植标本库收集有完整临床资料的501例肝移植供、受者组织及血液标本,其中肝癌标本203例,良性肝病标本214例,健康供肝标本84例。肝癌标本包括肿瘤组织、癌旁组织及远端非癌组织标本,共计1 773份;随机抽取45例标本进行质量监测,显示所收集的标本质量较好,开发了规范化的标本信息资料计算机管理系统。实验初步建立了具有一定规模的科研型肝癌肝移植标本库,有利于提高标本的质量,可操作性较好。


中国组织工程研究
杂志出版内容重点:肾移植肝移植移植;心脏移植;组织移植;皮肤移植;皮瓣移植;血管移植;器官移植组织工程


全文链接:

关键词: 移植, 组织工程, 生物样本库, 修复重建, 移植, 肝细胞肝癌, 肝移植, 规范化收集, 信息化管理, 国家自然科学基金

Abstract:

BACKGROUND: The establishment of a standardized clinical liver transplantation specimen bank is the primary condition for scientific research in this field, which can help to provide a qualified sample resource platform for research. 

OBJECTIVE: To primarily establish biological specimen bank of hepatocellular carcinoma for liver transplantation, to explore the standardized procedures of specimen collection, processing and preservation of hepatocellular carcinoma for liver transplantation, and to establish the sound and comprehensive information management system of clinical information of collected specimens.
METHODS: In accordance with standardized procedures to establish biological specimen banks, the operational processes and quality control system were formulated. Liver tissue and blood samples of hepatocellular carcinoma recipients undergoing liver transplantation were regularly collected, managed and stored. Simultaneously, liver tissue and blood samples of benign liver disease in liver transplant recipients and of healthy donor were collected as controls. A systematic management was conducted in collected specimens and corresponding clinical information.
RESULTS AND CONCLUSION: From August 2009, tissue and blood samples of 501 cases of receipts and donors undergoing liver transplantation with complete clinical information were collected from the specimen bank, including 203 hepatocellular carcinoma specimens, 214 benign liver disease specimens and 84 healthy donor specimens. These specimens included tumor tissue, adjacent tissues and distal non-cancerous tissue specimens, totally 1 773. A total of 45 specimens were randomly selected for quality monitoring. The collected specimens had a high quality. Specimen information data computer management system was developed. This study initially established a standardized research-based clinical transplantation specimen bank, which is helpful to elevate sample quality and has a good manipuility.


中国组织工程研究
杂志出版内容重点:肾移植肝移植移植;心脏移植;组织移植;皮肤移植;皮瓣移植;血管移植;器官移植组织工程


全文链接:

Key words:  liver neoplasms, liver transplantation, biological specimen banks

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