中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (43): 6907-6913.doi: 10.3969/j.issn.2095-4344.2014.43.004

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

微骨折与自体骨髓间充质干细胞外基质支架修复猪膝关节软骨缺损

李祥全，唐  成，宋科荣，金成哲   

  1. 南京医科大学附属南京医院，江苏省南京市  210006
  • 收稿日期:2014-07-23 出版日期:2014-10-15 发布日期:2014-10-15
  • 通讯作者: 金成哲，博士，副主任医师，南京医科大学附属南京医院，江苏省南京市 210006
  • 作者简介:李祥全，男，1989年生，江苏省徐州市人，汉族， 2014年南京医科大学毕业，硕士，医师，主要从事软骨再生相关研究。
  • 基金资助:

    江苏省医学重点人才项目(RC2011017)

Micro-fracture enhanced by autologous bone marrow mesenchymal stem cells extracellular matrix scaffold to treat articular cartilage defects in the knee of pigs 

Li Xiang-quan, Tang Cheng, Song Ke-rong, Jin Cheng-zhe   

  1. Nanjing Hospital of Nanjing Medical University, Nanjing 210006, Jiangsu Province, China
  • Received:2014-07-23 Online:2014-10-15 Published:2014-10-15
  • Contact: Jin Cheng-zhe, M.D., Associate chief physician, Nanjing Hospital of Nanjing Medical University, Nanjing 210006, Jiangsu Province, China
  • About author:Li Xiang-quan, Master, Physician, Nanjing Hospital of Nanjing Medical University, Nanjing 210006, Jiangsu Province, China
  • Supported by:

    the Key Medical Personnel Projects of Jiangsu Province, No. RC2011017

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摘要：

背景：微骨折术方法简单，操作方便，是治疗关节软骨缺损有效的方法之一，但仍然存在再生软骨为纤维软骨、再生软骨退化等问题。现在学者们主要致力于使用各种方法改良微骨折修复软骨缺损的效果。
目的：探索微骨折处理软骨缺损区域后植入自体骨髓间充质干细胞外基质支架治疗猪膝关节软骨缺损的疗效。
方法：分离并原代培养猪骨髓间充质干细胞，收集其分泌的细胞外基质膜，采用交联、冻干技术将收集的基质膜制备成三维多孔支架。选取小型成年猪，制备双膝股骨髁、股骨滑车部全层软骨缺损模型，深2 mm，直径6 mm；采用自体左右对照模式，右膝作为对照组，使用单纯微骨折治疗软骨缺损，左膝作为实验组，采用微骨折处理软骨缺损区域后，植入预先制备的支架。术后6个月使用番红固绿染色、Masson染色等评价软骨再生情况，使用Wakitani评分整体评估再生软骨，并测定再生组织糖胺聚糖、DNA含量。
结果与结论：术后6个月，实验组股骨滑车和股骨髁处均可见软骨修复，表面光滑，对照组股骨滑车修复组织表面较平整，股骨髁未见明显修复。实验组股骨滑车和股骨髁再生软骨经番红固绿染色、Masson染色均显示软骨层基质含量丰富，软骨下骨骨小梁密集，对照组软骨层染色不明显，软骨下骨修复欠佳。实验组Wakitani评分、糖胺聚糖含量高于对照组，DNA含量低于对照组(P < 0.05)。结果可见微骨折结合自体骨髓间充质干细胞外基质支架修复软骨效果良好，股骨滑车和股骨髁治疗效果无显著差异。


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

关键词: 生物材料, 软骨生物材料, 骨髓间充质干细胞, 细胞外基质, 微骨折术, 软骨缺损, 组织工程, 支架

Abstract:

BACKGROUND: Micro-fracture surgery method is simple, easy to operate, which is an effective way to treat articular cartilage defects, but there are still some problems such as regenerated fibrocartilage and regenerated cartilage degradation. Scholars have focused on the use of various methods to improve the micro-fracture effect on repairing cartilage defects.
OBJECTIVE: To explore the effects of micro-fracture enhanced by autologous bone marrow mesenchymal stem cells extracellular matrix (aBMSC-dECM) scaffold for treating cartilage defects in minipig models.
METHODS: Bone marrow was extracted from the minipigs and bone marrow mesenchymal stem cells were obtained. aBMSC-dECM membranes were collected. Cross-linking and freeze-drying technology were used to make the three-dimensional porous aBMSC-dECM scaffold. Full thickness cartilage defects, 2 mm in depth and 6 mm in diameter, were created on the femoral condyles and trochlea grooves of the two knees of the minipigs. The right knees were treated with micro-fracture as control and the left were treated with micro-fracture enhanced by aBMSC-dECM scaffold. Six months later, histological examination and Wakitani score were used to evaluate the cartilage regeneration, and glycosaminoglycans and DNA contents in the regenerative tissue were determined.
RESULTS AND CONCLUSION: After 6 months, the tissue treated by micro-fracture enhanced by aBMSC-dECM scaffold got better surface and integrated with the surrounding cartilage. Safranin O and fast green staining and Masson staining showed that the regenerated cartilage of the left knee, with abundant matrix and dense bone trabeculae, was better than that of the right. Wakitani score of the left knee was higher than that of the right. Glycosaminoglycans content of the left knee was much more than that of the right, while the DNA content was lower in the left knee than the right knee. Better results were observed in the left knee undergoing micro-fracture enhanced by aBMSC-dECM scaffold, and improvements in the femoral condyles and trochlea grooves showed no differences.


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: bone marrow, mesenchymal stem cells, extracellular matrix, cartilage, articular

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