中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (2): 171-176.doi: 10.3969/j.issn.2095-4344.2014.02.002

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

不同方向关节软骨缺损时软骨修复及膝关节运动功能恢复有无差异？

耿晓鹏1，王  霞2，杨  震1，窦永峰1，朱  锴1，房清敏1，孙兆忠1，李建军1   

  1. 1滨州医学院附属医院脊柱外科，山东省滨州市  256603；2滨州市人民医院，山东省滨州市  256610
  • 收稿日期:2013-10-14 出版日期:2014-01-08 发布日期:2014-01-08
  • 通讯作者: 杨震，在读硕士，滨州医学院附属医院脊柱外科，山东省滨州市
  • 作者简介:耿晓鹏，男，1970年生，汉族，2006年河北医科大学毕业，博士，副主任医师，硕士生导师，主要从事脊柱外科方面的研究。
  • 基金资助:

    滨州医学院科研启动基金资助项目(BY2006KYQD07)

Cartilage repair and motor function recovery in knee joint during articular cartilage defects at different directions

Geng Xiao-peng1, Wang Xia2, Yang Zhen1, Dou Yong-feng1, Zhu Kai1, Fang Qing-min1, Sun Zhao-zhong1, Li Jian-jun1   

  1. 1Department of Spinal Surgery, Affiliated Hospital, Binzhou Medical University, Binzhou 256603, Shandong Province, China; 2Binzhou Municipal People’s Hospital, Binzhou 256610, Shandong Province, China
  • Received:2013-10-14 Online:2014-01-08 Published:2014-01-08
  • Contact: 256603Yang Zhen, Studying for master’s degree, Department of Spinal Surgery, Affiliated Hospital, Binzhou Medical University, Binzhou 256603, Shandong Province, China
  • About author:Geng Xiao-peng, M.D., Associate chief physician, Master’s supervisor, Department of Spinal Surgery, Affiliated Hospital, Binzhou Medical University, Binzhou 256603, Shandong Province, China
  • Supported by:

    the Science Research Initial Project of Binzhou Medical University, No. BY2006KYQD07

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摘要：

背景：目前关于关节软骨缺损修复的研究方法较多，其中微骨折技术已得到普遍应用。
目的：观察不同方向(冠状位与矢状位)关节软骨缺损时膝关节运动功能恢复及组织修复形态学变化情况。
方法：建立兔膝关节股骨内侧髁全厚2 mm关节软骨缺损模型，根据切口方向分为冠状位组与矢状位组。分别在造模5，10，20周时行大体观察，取标本做石蜡切片后行苏木精-伊红染色、胶原蛋白染色，并应用光学显微镜和免疫组织学检查观察关节软骨缺损处的组织修复情况。造模后定期检查2组兔的关节活动度。
结果与结论：2组缺损处均可见越过股骨髁的一条白线，缺损修复的关节面与原位软骨平齐，并达到了骨性愈合，手术处理的膝关节对功能无影响。光学显微镜下2组均可见软骨下骨的部分重建，主要由纤维软骨修复，骨重建的水平低于邻近的原位软骨的潮线。免疫组织学检查可见2组标本的缺损处均呈现Ⅰ型胶原染色逐步减弱，Ⅱ型胶原染色逐步增强。提示不同方向(冠状位与矢状位)关节软骨缺损的组织修复及膝关节运动功能恢复无明显差异。



中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

关键词: 组织构建, 软骨组织工程, 关节软骨, 缺损, 冠状位, 矢状位, 病理学, 免疫组织学

Abstract:

BACKGROUND: At present, there are many researches about repairing articular cartilage defects. In particular, the microfracture technique has been widely used.
OBJECTIVE: To observe recovery of knee joint motor function and morphological changes in tissue repair during articular cartilage defects with different directions (coronal position and sagittal position).
METHODS: Articular cartilage fracture models with 2 mm-thick medial femoral condyles of rabbit knee joint were established. According to incision directions, models were assigned to coronal and sagittal groups. At 5, 10 and 20 weeks after model induction, general observation was performed. Specimens were sliced into paraffin sections, and subjected to hematoxylin-eosin staining and collagen staining. Tissue repair at the articular cartilage defects was observed using optical microscope and immunohistochemical method. After model induction, range of motion of rabbit joints was regularly examined in the two groups. 
RESULTS AND CONCLUSION: A white line was seen across the femoral condyles at defects in the two groups. Articular surface at defect repair was at the level of in situ cartilage, and reached a bone union. Knee joint treated by operation did not affect function. Under light microscope, partial reconstruction of subchondral bone was seen in the two groups, mainly fibrocartilage repair. The level of bony remodeling was lower than tidal line of adjacent in situ cartilage. Immunohistochemical method exhibited that type I collagen staining gradually reduced at defects of specimens, but type II collagen staining gradually increased. These results suggested that there was no significant difference in the recovery of motor function of knee joint and the repair of articular cartilage with different directions (coronal and sagittal position).



中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

Key words: cartilage, joints, wounds and injuries, knee joint, models, animal

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