中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (34): 6061-6066.doi: 10.3969/j.issn.2095-4344.2013.34.001

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials •    下一篇

复合同种异体成骨细胞的β-磷酸三钙人工骨修复桡骨缺损

杨华清1,张明宇2,党晓谦3,王坤正3   

  1. 1北京康复中心骨科,北京市  100144;2西安交通大学医学院附属红会医院运动损伤科,陕西省西安市  710054;3西安交通大学医学院第二附属医院骨科,陕西省西安市  710004
  • 出版日期:2013-08-20 发布日期:2013-08-20
  • 通讯作者: 张明宇,博士,西安交通大学医学院附属红会医院运动损伤科,陕西省西安市 710054 zhangmingyu2005@163.com
  • 作者简介:杨华清☆,男,1972年生,黑龙江省宁安市人,汉族,博士,骨科主任,擅长骨延长、骨再生、马蹄内翻足矫治,主要从事骨再生与肢体重建研究。 yhq402@126.com
  • 基金资助:

    教育部博士点基金项目(20070698083)。

Allogeneic osteoblasts compounded with beta-tricalcium phosphate for repair of radial defects

Yang Hua-qing1, Zhang Ming-yu2, Dang Xiao-qian3, Wang Kun-zheng3   

  1. 1 Department of Orthopedics, Beijing Rehabilitation Center, Beijing  100144, China; 2 Department of Sport Injury, Hong Hui Hospital, Xi’an Jiaotong University College of Medicine, Xi’an  710054, Shaanxi Province, China; 3 Department of Orthopedics, the Second Affiliated Hospital of Xi’an Jiaotong University College of Medicine, Xi’an  710004, Shaanxi Province, China
  • Online:2013-08-20 Published:2013-08-20
  • Contact: Ming-yu, M.D., Department of Sport Injury, Hong Hui Hospital, Xi’an Jiaotong University College of Medicine, Xi’an 710054, Shaanxi Province, China zhangmingyu2005@163.com
  • About author:Yang Hua-qing☆, M.D., Department of Orthopedics, Beijing Rehabilitation Center, Beijing 100144 , China yhq402@126.com
  • Supported by:

    Doctoral Fund of Ministry of Education of China, No. 20070698083*

摘要:

背景:作为骨支架材料,β-磷酸三钙具有良好的生物相容性、骨诱导性及生物力学性能。
目的:探讨β-磷酸三钙复合同种异体成骨细胞修复兔桡骨节段性骨缺损的效果。
方法:建立45只兔单侧桡骨骨缺损模型,随机分为3组,实验组缺损区植入复合同种异体成骨细胞的β-磷酸三钙,对照组缺损区植入β-磷酸三钙,空白对照组缺损区不植入任何材料。植入后4,8,16周观察新骨生成情况,通过形态学、X射线等观察指标客观评价各组成骨及骨缺损修复能力。
结果与结论:随着修复时间的延长,实验组骨缺损逐渐修复,至16周时X射线见支架与宿主骨之间的骨痂已完全骨化,骨缺损完全修复;组织学见缺损区皮质骨连续,髓腔再通,并且不同时间点实验组修复效果明显优于对照组与空白对照组(P < 0.01)。表明复合同种异体成骨细胞的β-磷酸三钙人工骨具有良好的成骨能力,有引导组织再生、防止骨不连的作用。

关键词: 生物材料, 组织工程骨材料, β-磷酸三钙, 人工骨, 成骨细胞, 骨缺损, 部级基金

Abstract:

BACKGROUND: As a bone scaffold material, β-tricalcium phosphate has good biocompatibility, osteoinductive, and biomechanical properties.
OBJECTIVE: To study the effect of allogeneic osteoblasts compounded with β-tricalcium phosphate in repairing rabbit radial defects.
METHODS: A total of 45 rabbit radial defect models were made and divided into three groups in random. Experimental group was repaired with the compound of allogeneic osteoblasts and β-tricalcium phosphate; control group with β-tricalcium phosphate; and blank control group with nothing. The new bone formation of each group was observed and assessed by X-ray and histopathological analysis at weeks 4, 8, 16 after implantation for evaluation of the bone repairing effect.
RESULTS AND CONCLUSION: With the repair time, the experimental group appeared to complete bone defect repair gradually. By the end of 16 weeks, the X-ray showed that the bone callus between the scaffold and the host was completely ossified, and bone defects were completely repaired in the experimental group. Histopathological observation showed continuous cortical bone formed in the defect area, and canal recanalization realized in the experimental group. Additionally, the repair effect in the experimental group was better than that in the control and blank control group at different time points (P < 0.01). It is suggested that the allogenic osteoblasts/β-tricalcium phosphate compound has the better effects on guiding bone regeneration and preventing from nonunion

Key words: biomaterials, tissue-engineered bone materials, β-tricalcium phosphate, artificial bone, osteoblasts, bone defect, ministerial grants-supported paper

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