中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (42): 7853-7856.doi: 10.3969/j.issn.2095-4344.2012.42.012

• 口腔组织构建 oral tissue construction • 上一篇    下一篇

葛根素在兔牙周组织改建中的作用

金珊丹，郑 嵘，吴立鹏，杨东红   

  1. 佳木斯大学附属口腔医院，黑龙江省佳木斯市 154002
  • 收稿日期:2012-03-21 修回日期:2012-04-09 出版日期:2012-10-14 发布日期:2012-10-14
  • 通讯作者: 吴立鹏，教授，佳木斯大学附属口腔医院，黑龙江省佳木斯市 154002
  • 作者简介:金珊丹★，女，1982年生，黑龙江省拜泉县人，汉族，2012年佳木斯大学口腔医学院毕业，硕士，主要从事口腔正畸研究。 Gzcjsd0606@163.com

Effect of puerarin on rabbit periodontal tissue remodeling

Jin Shan-dan, Zheng Rong, Wu Li-peng, Yang Dong-hong   

  1. Affiliated Stomatological Hospital of Jiamusi University, Jiamusi 154002, Heilongjiang Province, China
  • Received:2012-03-21 Revised:2012-04-09 Online:2012-10-14 Published:2012-10-14
  • Contact: Wu Li-peng, Professor, Affiliated Stomatological Hospital of Jiamusi University, Jiamusi 154002, Heilongjiang Province, China
  • About author:Jin Shan-dan, Master, Affiliated Stomatological Hospital of Jiamusi University, Jiamusi 154002, Heilongjiang Province, China Gzcjsd0606@163.com

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摘要：

背景：研究发现葛根素对体外骨形成具有一定的促进作用。
目的：葛根素在牙周组织改建中的作用。
方法：33只兔随机分成空白对照组3只、阴性对照组15只和和实验组15只。给兔双侧下颌第1磨牙安装加力装置，均加力14 d结束后，安装保持器装置，保持期对照组耳缘静脉注射生理盐水，实验组给予葛根素注射液。分别于加力保持1，3，7，14，21 d，每个时间点各处死3只，取组织标本。
结果与结论：实验组：第1天远中侧可见新骨形成，边缘有成骨细胞，近中侧可见较多的破骨细胞；第14，21天近远中侧均明显的新骨堆积，致密的骨小梁形成。阴性对照组：第1天组与实验组类似；第14天近远中侧均有新骨形成，见未完全钙化的类骨质，骨吸收陷窝消失，表面见成骨细胞；第21天近远中侧均见大量新骨形成。与阴性对照组比较，各时间点实验组骨形态发生蛋白2的表达量高于阴性对照组，第7，14，21天两组表达量差异有显著性意义(P < 0.05)。实验组骨形态发生蛋白2的表达呈逐渐增强的趋势，阴性对照组骨形态发生蛋白2的表达呈逐渐减少的趋势。实验组与阴性对照组骨形态发生蛋白2的表达均强于空白对照组(P < 0.05)。提示葛根素可能在保持期通过上调骨形态发生蛋白2的表达而促进成骨细胞抑制破骨细胞从而缩短保持期时间。

关键词: 葛根素, 正畸保持期, 骨形态发生蛋白2, 成骨细胞, 破骨细胞, 组织构建

Abstract:

BACKGROUND: Several studies have shown that puerarin has promoting effect on bone formation in vitro.
OBJECTIVE: To investigate the effect of puerarin on rabbit periodontal tissue remodeling.
METHODS: Totally 33 rabbits were randomly divided into the blank control group (three rabbits), the negative control group (15 rabbits) and the experimental group (15 rabbits). Rabbits’ bilateral mandibular first molars were installed stressing device, and after 14 days, the rabbits were installed retainer device. During retention period, the rabbits in the control group were injected normal saline, while the rabbits in the experimental group were injected puerarin. The two groups were maintained for 1, 3, 7, 14 and 21 days, respectively. Three rabbits were killed at each time point and their tissue samples were extracted.
RESULTS AND CONCLUSION: In the experimental group: new bone formed in the far side, and there were osteoblasts at the verge, in addition, more osteoclasts were found in the near side at first day; at days 14 and 21, obvious accumulation of new bone was found in the near and far side, and dense trabecular bone formed. In the negative control group: at first day, it was similar to the experimental group; at day 14, there were new bones formed in the near and far side, and completely calcified osteoid was not be seen, besides, lacunar resorption disappeared, and osteoblasts were found on the surface; at day 21, a number of new bones formed in the near and far side. Compared with the negative control group, bone morphogenetic protein-2 (BMP-2) expression of the experimental group was higher at each time point. The express levels between the two groups at days 7, 14 and 21 had significant difference (P < 0.05). The BMP-2 expression of the experimental group was increased gradually, while that of the negative control group was decreased gradually. The BMP-2 expression of the experimental group and negative control group were both higher than that of the blank control group (P < 0.05). These results suggest that puerarin may promote osteoblast and inhibit osteoclast so as to shorten the retention period through the up-regulation of BMP-2 expression in retention period.

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