中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (37): 6942-6946.doi: 10.3969/j.issn.2095-4344.2012.37.021

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

整合素连接激酶高表达对新生大鼠心肌细胞增殖的影响

王丙剑1，徐 标2，白 剑2，李倩君1，孙静娴3，张 娜2   

  1. 1南京医科大学附属淮安第一医院，江苏省淮安市 223300
    2南京医科大学附属鼓楼临床医学院心脏科，江苏省南京市 210008
    3江苏省省级机关医院心脏科，江苏省南京市 210029
  • 收稿日期:2012-05-09 修回日期:2012-06-18 出版日期:2012-09-09 发布日期:2012-09-09
  • 作者简介:王丙剑★，男，1979年生，江苏省沭阳县人，汉族，2001年南京医科大学毕业，硕士，主治医师，主要从事冠心病及心力衰竭治疗方面的研究。 wangbingjian@medmail.com.cn

Effects of over-expressed integrin-linked kinase on rat neonatal cardiomyocyte proliferation

Wang Bing-jian1, Xu Biao2, Bai Jian2, Li Qian-jun1, Sun Jing-xian3, Zhang Na2   

  1. 1Huaian First Hospital of Nanjing Medical University, Huaian 223300, Jiangsu Province, China
    2Department of Cardiology, Drum Tower Clinical Medical College, Nanjing Medical University, Nanjing 210008, Jiangsu Province, China
    3Department of Cardiology, Jiangsu Province Official Hospital, Nanjing 210029, Jiangsu Province, China
  • Received:2012-05-09 Revised:2012-06-18 Online:2012-09-09 Published:2012-09-09
  • About author:Wang Bing-jian★, Master, Attending physician, Huaian First Hospital of Nanjing Medical University, Huaian 223300, Jiangsu Province, China wangbingjian@medmail.com.cn

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443

被引次数

1

摘要：

背景：整合素连接激酶在调节细胞生存、抗凋亡以及促进细胞迁移、增殖、分化等方面发挥重要作用。
目的：观察整合素连接激酶在新生大鼠心肌细胞内高表达后对心肌细胞增殖能力的影响。
方法：取新生3 d内SD大鼠心脏，体外分离、培养心肌细胞72 h后，分别转染重组腺病毒载体或重组腺病毒载体+整合素连接激酶基因。
结果与结论：转染48 h，与转染重组腺病毒载体比较，转染重组腺病毒载体+整合素连接激酶基因的新生大鼠心肌细胞内DNA合成增加(P < 0.05)，有丝分裂增多(P < 0.05)，心肌细胞数量增多(P < 0.05)。在整合素连接激酶的作用下，新生大鼠心肌细胞的增殖能力明显提高。

关键词: 整合素连接激酶, 基因转染, 基因治疗, 大鼠心肌细胞, 增殖能力, 有丝分裂, 心力衰竭

Abstract:

BACKGROUND: Integrin-linked kinase plays an important role in regulating cell survival and anti-apoptosis to promote cell migration, proliferation, and differentiation.
OBJECTIVE: To explore the effects of over-expressed integrin-linked kinase on cardiomyocytes proliferation in neonatal rats.
METHODS: The heart of neonatal rats (1–3 days old) was extracted to in vitro isolate and culture cardiomyocytes for 72 hours. Then, the cells were transfected with recombinant adenovirus vector or recombinant adenovirus vector+ integrin-linked kinase gene.
RESULTS AND CONCLUSION: Compared with the recombinant adenovirus vector group, there was an increase in DNA synthesis (P < 0.05), mitosis (P < 0.05) and the number of cardiomyocytes (P < 0.05) in the recombinant adenovirus vector+ integrin-linked kinase gene group. Briefly, integrin-linked kinase can promote the proliferation of neonatal rat cardiomyocytes.

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