中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (37): 6916-6920.doi: 10.3969/j.issn.2095-4344.2012.37.016

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

白藜芦醇调控体外培养人骨关节炎滑膜细胞白细胞介素18的表达

杨建辉，吕建国，聂会勇，申晓东   

  1. 西安交通大学第一医院疼痛科，陕西省西安市 710061
  • 收稿日期:2011-11-15 修回日期:2011-12-20 出版日期:2012-09-09 发布日期:2012-09-09
  • 作者简介:杨建辉★，男，1964年生，河南省偃师市人，汉族，1998年西安交通大学医学院毕业，硕士，副主任医师，主要从事各类疼痛性疾病的临床研究。 hiuyang@sina.cn

Resveratrol down-regulates the expression of interleukin-18 in syoviocytes of osteoarthritis cultured in vitro

Yang Jian-hui, Lü Jian-guo, Nie Hui-yong, Shen Xiao-dong   

  1. Department of Pain, First Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China
  • Received:2011-11-15 Revised:2011-12-20 Online:2012-09-09 Published:2012-09-09
  • About author:Yang Jian-hui★, Master, Associate chief physician, Department of Pain, First Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China hiuyang@sina.cn

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被引次数

1

摘要：

背景：白藜芦醇可在体外抑制软骨细胞的凋亡。
目的：观察白藜芦醇对体外培养人骨关节炎滑膜细胞中白细胞介素18、白细胞介素1β和肿瘤坏死因子α表达的影响。
方法：对兔以白藜芦醇临床等效剂量灌胃后，制备含10%，20%，40%白藜芦醇含药血清，与人骨关节炎滑膜细胞共培养，以正常兔血清培养细胞为对照。
结果与结论：ELISA检测及免疫细胞化学检测结果显示，不同浓度白藜芦醇含药血清组体外培养滑膜细胞白细胞介素18、白细胞介素1β、肿瘤坏死因子α分泌量较正常兔血清组明显降低(P < 0.01)，并随白藜芦醇浓度的增加，白细胞介素18、白细胞介素1β、肿瘤坏死因子α分泌量逐渐降低(P < 0.01或P < 0.05)。白细胞介素1β、肿瘤坏死因子α水平依次与白细胞介素18水平呈正相关。表明白藜芦醇能够显著下调白细胞介素18、白细胞介素1β、肿瘤坏死因子α在骨关节炎滑膜细胞中的表达，减轻滑膜炎症反应。

关键词: 骨关节炎, 滑膜细胞, 细胞因子, 白藜芦醇, 白细胞介素18, 白细胞介素1β, 肿瘤坏死因子α

Abstract:

BACKGROUND: Resveratrol can inhibit chondrocytes apoptosis in vitro.
OBJECTIVE: To observe the effect of Res on the expression of interleukin-18, interleukin-1 beta and tumor necrosis factor-alpha in synoviocytes of osteoarthritis cultured in vitro.
METHODS: After the rabbits were lavaged with clinical equivalent dose of resveratrol, the 10%, 20% and 40% resveratrol-containing serum were prepared, and then co-cultured with the osteoarthritis synoviocyte. The normal rabbit culture serum was as control.
RESULTS AND CONCLUSION: Enzyme linked immunosorbent assay detection and immunocytochemistry results showed that the secretion of interleukin-18, interleukin-1 beta and TNF-α of synoviocyte cultured in vitro in resveratrol-containing serum group was significant lower than that in the non-resveratrol containing serum group (P < 0.01), and with the increasing of resveratrol concentration, the level of interleukin-18, interleukin-1 beta and tumor necrosis factor-alpha secretion was gradually reduced (P < 0.01 or P < 0.05). There was a positive correlation between interleukin-18, tumor necrosis factor-alpha and interleukin-1 beta. It indicates that resveratrol can down-regulate the expression of interleukin-18, interleukin-1 beta and tumor necrosis factor-alpha in synoviocyte and reduce the inflammatory reaction.

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