中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (32): 6011-6013.doi: 10.3969/j.issn.2095-4344.2012.32.023

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

红细胞冰冻前的最佳保存期探讨

王赤林，谢毓滨，邱 明，旷开其，钟待鸣   

  1. 长沙血液中心，湖南省长沙市 410001
  • 收稿日期:2011-12-03 修回日期:2012-02-24 出版日期:2012-08-05 发布日期:2012-08-05
  • 作者简介:王赤林，女，1953年生，湖南省常德市人，汉族，湖南医科大学师资本科班医学检验专业毕业，主任检验技师，主要从事输血和血液检测方面的研究。 wangchilin6@sina.com

Best storage time for red blood cells prior to freezing

Wang Chi-lin, Xie Yu-bin, Qiu Ming, Kuang Kai-qi, Zhong Dai-ming   

  1. Changsha Blood Center, Changsha 410001, Hunan Province, China
  • Received:2011-12-03 Revised:2012-02-24 Online:2012-08-05 Published:2012-08-05
  • About author:Wang Chi-lin, Chief technician, Changsha Blood Center, Changsha 410001, Hunan Province, China wangchilin6@sina.com

PDF

417

被引次数

2

摘要：

背景：既能适当延长冰冻红细胞在冰冻前的保存期，又能保证冰冻红细胞的质量，是十分有意义的研究课题。
目的：适当延长稀有血型红细胞冰冻前在(4±2) ℃的保存期，满足急症稀有血型患者的输血，降低血液成本，减轻工作强度。
方法：将冰冻前(4±2) ℃保存1~6 d和冰冻前(4±2) ℃保存7~12 d制备的冰冻红细胞进行质量比较；根据血液保养液的红细胞保护原理、美国AABB相关标准及质检结果将含添加剂的红细胞在冰冻前的保存期设定为自采血之日起14 d内，对采取此措施前后2001/2009本中心的Rh(D)阴性供血情况进行统计分析。
结果与结论：质量检测结果达到国家标准，两组质量检测数据比较差异无显著性意义；将含添加剂的红细胞在冰冻前的保存期设定为自采血之日起14 d内，本中心Rh(D)阴性血年供血量虽逐年增加，但冰冻红细胞的供血量却未增加，其占供血的比例还在逐年下降，(4±2) ℃保存血的供血比例则逐渐增加。冰冻前在(4±2) ℃保存14 d再制备的冰冻红细胞发往临床均未发现因此而产生的血液质量问题。结果可见增加Rh(D)阴性(4±2) ℃保存血供血量，减少冰冻红细胞供血量，能及时满足急症Rh(D)阴性患者的输血，降低制备冰冻红细胞导致的高血液成本，减轻员工制备冰冻红细胞的工作强度。

关键词: 冰冻红细胞, 冰冻前, 最佳保存期, Rh(D)阴性, (4±2) ℃

Abstract:

BACKGROUND: Appropriately extending the storage time of red blood cells prior to freezing and ensuring the quality of frozen red blood cells are beneficial for blood use in clinical emergency and can decrease blood costs.
OBJECTIVE: To appropriately extend the storage time at (4±2) ℃ for rare-type red blood cells prior to freezing, to meet the needs of emergency blood transfusion in patients with rare blood types, decrease blood costs, and to reduce the work intensity.
METHODS: A quality comparison was performed between red blood cells stored at (4±2) ℃ for 1-6 days and red blood cells stored at (4±2) ℃ for 7-12 days. According to the principle of blood preservative solution to protect red blood cells, the U.S. AABB related standards as well as the quality control results, the storage time for additive-containing red blood cells prior to freezing should be set within 14 days from the date of blood collection. Rh(D)-negative blood supplied by our center between 2001 and 2009 was statistically analyzed before and after taking this measure.
RESULTS AND CONCLUSION: Quality test results met the national standards and two sets of quality control data showed no significant statistical difference. The storage time for red blood cells with additives prior to freezing was set within 14 days after the date of blood collection. Although the annual blood supply of Rh(D)-negative blood of our center increased year by year, the supply of frozen red blood cells was not increased and its proportion in the total blood supply decreased yearly, while the proportion of blood supply for blood stored at (4±2) ℃ increased gradually. No clinical blood quality problems were found regarding the red blood cells which were stored at (4±2) ℃ for 14 days prior to freezing. Results showed that increasing the blood supply of Rh(D)-negative blood stored at (4±2) ℃ and decreasing the blood supply of frozen red blood cells can meet the needs of emergency blood transfusion of Rh D)-negative patients in time, lower the high blood costs for preparation of frozen red blood cells, and reduce the work intensity for staffs in preparing the frozen red blood cells.

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