中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (32): 5915-5919.doi: 10.3969/j.issn.2095-4344.2012.32.005

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

脯氨酸羟化酶抑制剂可抑制小鼠骨髓间充质干细胞凋亡

张 磊，赵学凌，李 彪，刘劲松，李 溪，龚跃昆   

  1. 昆明医学院第一附属医院骨科，云南省昆明市 650032)
  • 收稿日期:2011-10-11 修回日期:2011-12-01 出版日期:2012-08-05 发布日期:2012-08-05
  • 通讯作者: 龚跃昆，主任医师，昆明医学院第一附属医院骨科，云南省昆明市 650032
  • 作者简介:张磊☆，男，1981年生，四川省德阳市人，昆明医学院在读博士，主要从事骨科基础和临床研究。

Prolyl hydroxylase inhibitor prevents bone marrow mesenchymal stem cells apoptosis in mice

Zhang Lei, Zhao Xue-ling, Li Biao, Liu Jin-song, Li Xi, Gong Yue-kun   

  1. Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China
  • Received:2011-10-11 Revised:2011-12-01 Online:2012-08-05 Published:2012-08-05
  • Contact: Gong Yue-kun, Chief physician, Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China gongyuekun@yahoo.com.cn
  • About author:Zhang Lei☆, Studying for doctorate, Department of Orthopedics, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China lzhang670252@163.com

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摘要：

背景：脯氨酸羟化酶抑制剂能够通过稳定低氧诱导因子使其下游基因表达上调，进而对细胞凋亡发挥调控作用。
目的：观察脯氨酸羟化酶抑制剂二甲基乙二酰基甘氨酸对无血清培养引起的小鼠骨髓间充质干细胞凋亡的影响。
方法：分离传代培养并鉴定小鼠骨髓间充质干细胞。分别应用无血清(无血清对照组)及含体积分数10%胎牛血清(正常对照组)的DMEM培养基培养小鼠骨髓间充质干细胞24，48，72 h，实验组在无血清对照组的基础上给予1 mmol/L的二甲基乙二酰基甘氨酸。
结果与结论：实验所获的细胞通过流式细胞仪细胞表型鉴定和三系分化鉴定证实为骨髓间充质干细胞。流式细胞仪检测结果显示，与无血清对照组比较，实验组骨髓间充质干细胞凋亡率明显降低(P < 0.01)。说明脯氨酸羟化酶抑制剂二甲基乙二酰基甘氨酸可拮抗血清剥夺引起的骨髓间充质干细胞凋亡。

关键词: 脯氨酸羟化酶抑制剂, 二甲基乙二酰基甘氨酸, 骨髓间充质干细胞, 凋亡

Abstract:

BACKGROUND: Prolyl hydroxylase inhibitors can stabilize hypoxia inducible factor to up-regulate downstream genes expression, thereby regulating cell apoptosis.
OBJECTIVE: To evaluate the effects of the prolyl hydroxylase inhibitors dimethyloxalglycine (DMOG) on apoptosis of bone marrow-derived mesenchymal stem cells (BMSCs) induced by serum deprivation.
METHODS: Bone marrow mononuclear cells were harvested from Kunming mice bone marrow cavity of the femur and tibia and subcultured. The passage five mononuclear cells were identified by flow cytometry and tri-lineage differentiation. Bone marrow mononuclear cells were cultured in serum-free DMEM for serum deprivation (serum-deprivation group) and DMEM containing 10% fetal bovine serum (normal control group) for 24, 48, and 72 hours. Experimental group was treated with 1 mmol/L DMOG based on serum-free culture.
RESULTS AND CONCLUSION: The bone marrow mononuclear cells from the mice were purified through subculture and were verified as BMSCs through cell surface marker identification by flow cytometry. Compared with serum-deprivation group, the apoptosis rate was significantly decreased in experimental group (P < 0.01). Results indicate that DMOG can suppress BMSCs apoptosis induced by serum deprivation.

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