中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (28): 5227-5231.doi: 10.3969/j.issn.2095-4344.2012.28.020

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

针刺提高2型糖尿病模型大鼠胰岛β细胞胰岛素的表达

孙 志1，马 丽1，韩海荣2，张作记2，孔令斌2   

  1. 1南京中医药大学针药结合实验室，江苏省南京市 210029；
    2济宁医学院，山东省济宁市 274300
  • 收稿日期:2011-12-09 修回日期:2011-12-27 出版日期:2012-07-08 发布日期:2012-07-08
  • 作者简介:孙志☆，男，1962年生，山东省单县人，汉族，2003年南京中医药大学毕业，博士，主任医师，教授，主要从事针灸治疗内分泌代谢病研究。 sunzhi63@163.com

Improved effect of acupuncture on insulin expression of islet beta cells in type 2 diabetes rats

Sun Zhi1, Ma Li1, Han Hai-rong2, Zhang Zuo-ji2, Kong Ling-bin2   

  1. 1Laboratory of Integrated Acupuncture and Medicine, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China;
    2Jining Medical College, Jining 274300, Shandong Province, China
  • Received:2011-12-09 Revised:2011-12-27 Online:2012-07-08 Published:2012-07-08
  • About author:Sun Zhi,☆ Doctor, Chief physician, Professor, Laboratory of Integrated Acupuncture and Medicine, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China sunzhi63@163.com

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摘要：

背景：针刺治疗2型糖尿病具有很好的疗效。
目的：观察针刺对2型糖尿病模型大鼠胰岛β细胞胰岛素表达的影响。
方法：将糖尿病模型大鼠按随机化原则分为针刺组、西药组、模型组，同时设置正常对照组。针刺组大鼠取足三里、内庭和胰俞穴给予针刺治疗，西药组用罗格列酮0.2 mg/kg灌胃，模型组用双蒸水2 mL/kg灌胃，均1次/d，连续4周。
结果与结论：①血糖：针刺组明显低于模型组、西药组(P < 0.05)。②血脂：针刺组胆固醇和三酰甘油低于模型组 (P < 0.05)；高密度脂蛋白胆固醇明显高于模型组(P < 0.01)。针刺组三指标与西药组比较差异无显著性意义。③胰岛β细胞胰岛素表达：针刺组显著高于模型组和西药组(P < 0.05)。④胰岛形态：模型组与西药组胰岛结构不完整，结构破坏，胰岛素染色效果差；针刺组胰岛结构趋向完整，胰岛素染色颗粒明显。说明针刺可显著提高2型糖尿病模型大鼠胰岛β细胞胰岛素表达水平，有效改善胰岛β细胞功能。

关键词: 针灸, 2型糖尿病模型, 胰岛β细胞, 胰岛素表达, 大鼠

Abstract:

BACKGROUND: Acupuncture for type 2 diabetes has obtained good clinical effects.
OBJECTIVE: To observe the effect of acupuncture on insulin expression of islet beta cells in type 2 diabetes rats.
METHODS: The diabetes model rats were randomly divided into acupuncture, rosiglitazone, model and normal control groups. Acupoints of Zusanli, Neiting and Yishu were selected in the acupuncture group for needling. Rosiglitazone (0.2 mg/kg) were given intragastrically in the rosiglitazone group. Double distilled water (2 mL/kg) was given intragastrically in the model group once daily for 4 consecutive weeks.
RESULTS AND CONCLUSION: ① Blood glucose level of the acupuncture group was significantly lower than that of the model and rosiglitazone groups (P < 0.05). ② Blood lipid: Cholesterol and triglyceride of the acupuncture group were significantly lower than those of the model group (P < 0.05), and high-density lipoprotein cholesterol of the acupuncture group was significantly higher than that of the model group (P < 0.01). The above three indicators between the acupuncture and rosiglitazone groups had no significant difference. ③ The insulin expression of islet beta cells in the acupuncture group was significantly higher than that of the model and rosiglitazone groups (P < 0.05). ④ Morphology of islet: Islet structure was incomplete and damaged, and the staining of insulin was poor in the model and rosiglitazone groups. Islet structure tended to be complete, and staining of insulin was visible in the acupuncture group. It is indicated that acupuncture can significantly increase the insulin expression and improve the function of islet beta cells in type 2 diabetes rats.

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