中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (20): 3195-3200.doi: 10.3969/j.issn.2095-4344.0320

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

甲强龙联合脂多糖诱导成年犬股骨头动-静脉系统的组织病理学特点

张  耀1，2，赵德伟1，2，王子华2，尉晓蔚2，田丰德2，杨  帆2   

  1. 1大连理工大学生物医学工程学院，辽宁省大连市  116024；2大连大学附属中山医院骨科，辽宁省大连市  116001
  • 收稿日期:2018-05-02 出版日期:2018-07-18 发布日期:2018-07-18
  • 通讯作者: 赵德伟，博士生导师，主任医师。大连理工大学生物医学工程学院，辽宁省大连市 116024；大连大学附属中山医院骨科，辽宁省大连市 116001
  • 作者简介:张耀，男，大连理工大学生物医学工程系在读博士，大连大学附属中山医院骨科主任医师，主要从事骨肿瘤骨病及生物医学材料的研究。
  • 基金资助:

    国家自然科学基金(81601885)

Histopathological characteristics of femoral head arteriovenous system induced by methylprednisolone and lipopolysaccharide in adult dogs

Zhang Yao1, 2, Zhao De-wei1, 2, Wang Zi-hua2, Yu Xiao-wei2, Tian Feng-de2, Yang Fan2   

  1. 1School of Biomedical Engineering, Dalian University of Technology, Dalian 116024, Liaoning Province, China; 2Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China
  • Received:2018-05-02 Online:2018-07-18 Published:2018-07-18
  • Contact: Zhao De-wei, Doctoral supervisor, Chief physician, School of Biomedical Engineering, Dalian University of Technology, Dalian 116024, Liaoning Province, China; Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China
  • About author:Zhang Yao, Doctoral candidate, Chief physician, School of Biomedical Engineering, Dalian University of Technology, Dalian 116024, Liaoning Province, China; Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81601885

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摘要：

文章快速阅读：

文题释义：
激素性股骨头坏死：是因为长时间使用激素而引起的一种股骨头坏死，从而引起骨细胞进一步缺血、坏死、骨小梁断裂、股骨头塌陷。
脂多糖：是革兰阴性细菌细胞壁中的一种成分，脂多糖对宿主是有毒性的。脂多糖只有当细菌死亡溶解或用人工方法破坏菌细胞后才释放出来，所以叫做内毒素。
摘要
背景：糖皮质激素类的诸多作用机制对于与骨坏死的病理过程的启动有密切关系，然而对于激素性骨坏死时股骨头动、静脉血管在不同时段发生的病理形态学变化的区别仍缺少相关记述。
目的：观察大剂量皮质激素联合脂多糖诱导下的成年犬在不同时段其动脉和静脉系统的组织病理形态学变化，以讨论这种变化对骨坏死发生和发展造成的可能影响。
方法：将健康成年犬15只随机分为4，8周观察组及对照组，每组5只(10髋)。4，8周观察组行静脉注射脂多糖10 g/kg，于24 h后肌注甲强龙20 mg/kg，连续注射3次，每次间隔24 h，干预后4，8周麻醉处死，取股骨头行双侧病理切片染色，对骨内及支持带动静脉血管缩窄度进行分析测量。
结果与结论：诱导4，8周后，犬骨内静脉的结构形态发生明显变化，表现为正常型静脉结构显著减少，而中度狭窄型、阻塞塌陷型静脉结构占比显著升高。在骨内动脉系统，8周观察组相对其他2组骨内区域动脉血管内膜相对增厚，血管缩窄程度高(P < 0.05)。结果证实，在皮质激素联合脂多糖诱导下，成年犬股骨头动脉和静脉系统出现病理变化的时间节点存在差异。静脉系统在药物诱导早期(4周)最先呈现出病理变化，而动脉系统病理变化出现在药物诱导8周后。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0002-4725-111X(张耀)

关键词: 股骨头坏死, 动物实验, 骨内动静脉, 糖皮质激素-脂多糖诱导, 组织学, 病理学, 血管缩窄度, 组织工程

Abstract:

BACKGROUND: Many mechanisms of action of glucocorticoids are closely related to the occurrence of osteonecrosis. However, the differences in pathomorphological changes between femoral head arteries and veins at different periods of steroid-induced osteonecrosis are rarely reported.
OBJECTIVE: To observe the histopathological changes of the arterial and venous system in adult dogs induced by high-dose corticosteroid combined with lipopolysaccharide, and to explore effects of these changes on the occurrence and development of osteonecrosis.
METHODS: Fifteen healthy adult dogs were randomly divided into, 4- and 8-week observation groups and control group (n=5 (10 hips) per group). The dogs in the observation groups underwent venous injection of 10 g/kg lipopolysaccharide, then intramuscular injection of 20 mg/kg methylprednisolone after 24 hours, for 3 times, with 24 hours in between. Afterwards, the dogs were sacrificed at 4 and 8 weeks respectively. The bilateral femoral heads were removed for pathological observation. The stenotic degree of arteries and veins in the bone and supporting band was measured.
RESULTS AND CONCLUSION: At 4 and 8 weeks after induction, the structure and morphology of normal intraosseous vein decreased significantly, while the proportion of moderate-stenotic and blocked-collapsed veins increased significantly. Compared with the 4-week observation and control groups, in the 8-week observation group, the thickness of arterial intima in the bone was significantly increased, and the stenotic degree was significantly increased (P < 0.05). These results indicate that there is difference in the occurrence of pathological changes between femoral artery and venous system in adult dogs under the induction of corticosteroids combined with lipopolysaccharide. The venous system presents the pathological changes at the early stage after induction (4 weeks), and the pathological changes occurred in the arterial system at 8 weeks.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Tissue Engineering, Femur Head Necrosis, Lipopolysaccharides

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