中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (4): 558-563.doi: 10.3969/j.issn.2095-4344.0090

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

天麻素对脊髓损伤模型大鼠神经功能恢复和胶质纤维酸性蛋白表达的影响

肖雪飞1，3，李娟娟2，黄  辉1，张  翔1，黄  鹞1，李经辉1，余化霖1   

  1. 1昆明医科大学第一附属医院神经外二科，云南省昆明市  650032；2昆明医科大学基础医学院组胚教研室，云南省昆明市  650500；3重庆医科大学第一附属医院璧山医院，重庆市  402760
  • 收稿日期:2017-09-12 出版日期:2018-02-08 发布日期:2018-02-08
  • 通讯作者: 李经辉，博士，硕士生导师，副主任医师，昆明医科大学第一附属医院神经外二科，云南省昆明市 650032 并列通讯作者：余化霖，博士，博士生导师，教授，主任医师，昆明医科大学第一附属医院神经外二科，云南省昆明市 650032
  • 作者简介:肖雪飞，男，1988年生，重庆市人，汉族，2017年昆明医科大学毕业，硕士，医师，主要从事创伤外科研究。
  • 基金资助:

    国家自然科学基金(81560206)；云南省应用基础研究项目(2017FE467-033)；云南省教育厅科学研究基金项目(2013Y287)

Gastrodin effects on the neural functional recovery and expression of glial fibrillary acidic protein in a rat model of spinal cord injury

Xiao Xue-fei1, 3, Li Juan-juan2, Huang Hui1, Zhang Xiang1, Huang Yao1, Li Jing-hui1, Yu Hua-lin1   

  1. 1Second Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China; 2Department of Histology and Embryology, School of Basic Medicine, Kunming Medical University, Kunming 650500, Yunnan Province, China; 3Bishan Hospital, the First Affiliated Hospital of Chongqing Medical University, Chongqing 402760, China
  • Received:2017-09-12 Online:2018-02-08 Published:2018-02-08
  • Contact: Li Jing-hui, M.D., Mater’s supervisor, Associate chief physician, Second Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China Corresponding author: Yu Hua-lin, M.D., Doctoral supervisor, Professor, Chief physician, Second Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China
  • About author:Xiao Xue-fei, Master, Physician, Second Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China; Bishan Hospital, the First Affiliated Hospital of Chongqing Medical University, Chongqing 402760, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81560206; the Applied Basic Research Project of Yunnan Province, No. 2017FE467-033; the Science Research Foundation of Education Department of Yunnan Province, No. 2013Y287

PDF

241

被引次数

1

摘要：

文章快速阅读：

文题释义：
天麻素：天麻素是从天然药物天麻中提取的一种酚甙类化合物，是天麻中的主要活性成分之一，具有高效、低毒，不易在体内蓄积的特点。研究表明天麻素可以透过血脑屏障，并分布于中枢神经系统，具有抑制星形胶质细胞活化、保护神经元的作用。
钳夹型脊髓损伤模型：通过使用固定钳夹力的医用颅内动脉瘤夹对大鼠脊髓进行钳夹损伤，其损伤机制和病理生理变化与临床类似，能够按照实验需求调整钳夹力度、角度、时间、节段部位和夹闭程度，可以自由模拟临床脊髓损伤中最常见的挤压损伤机制和病理生理变化，模型具有相似性、可重复性、可靠性和可控性等特点。

摘要
背景：研究表明，天麻素在体外可以抑制无血清损伤后星形胶质细胞活化，改善脊髓损伤微环境，减轻继发损伤。
目的：观察天麻素对钳夹型大鼠脊髓损伤模型神经功能恢复、胶质纤维酸性蛋白的影响。
方法：48只成年SD大鼠随机分为3组，脊髓损伤组建立钳夹型大鼠脊髓损伤模型；假手术组仅切除椎板；天麻素组造模后给予天麻素100 mg/(kg•d)治疗；假手术组和脊髓损伤组给予等量生理盐水。术后分别于1，3，7，14，21，28 d采用BBB运动功能评分和Rivlin斜板实验评估脊髓功能恢复情况，术后28 d进行灌注、取材，观察损伤节段脊髓形态变化、免疫荧光观察胶质纤维酸性蛋白表达变化、尼氏染色观察尼氏小体变化情况。
结果与结论：①造模后各组大鼠BBB评分和Rivlin斜板实验评分均下降，随着时间推移各组评分逐渐升高，假手术组评分于各时相点均高于其余各组(P < 0.05)，天麻素组在术后3 d及其后各时相点评分均明显均高于脊髓损伤组(P < 0.05)；②术后28 d，天麻素组和脊髓损伤组胶质纤维酸性蛋白表达较假手术组均明显增加(P < 0.05)，天麻素组胶质纤维酸性蛋白表达较脊髓损伤组明显降低(P < 0.05)；③天麻素组和脊髓损伤组Nissl小体计数均较假手术组少(P < 0.05)，染色较浅，部分Nissl小体溶解，天麻素组Nissl小体计数较脊髓损伤组明显增多(P < 0.05)；④假手术组脊髓组织结构完整，脊髓损伤组脊髓结构形态紊乱，脊髓空洞形成，灰白质界限不清，天麻素组脊髓空洞较小，灰白质界限尚清晰；⑤结果表明，天麻素可以在脊髓损伤后抑制星形胶质细胞活化增生和保护神经元，改善肢体运动功能。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0003-1824-9966(肖雪飞)

关键词: 天麻素, 神经损伤与修复动物模型, 钳夹型脊髓损伤, 运动功能, 星形胶质细胞, 神经元, 尼氏小体, 运动功能, 组织构建

Abstract:

BACKGROUND: Gastrodin has been shown to inhibit the in vivo astrocyte activation after injury, improve the microenvironment of spinal cord injury (SCI), and reduce secondary injuries.
OBJECTIVE: To observe the effects of gastrodine on the neural functional recovery and glial fibrillary acidic protein in a rat model of clamping-induced SCI.
METHODS: Forty-eight adult Sprague-Dawley rats were randomly divided into three groups, and the models of clamping-induced SCI were established. The rats in sham group were only subjected to laminectomy; gastrodine group was given 100 mg/kg•d gastrodine; the sham and SCI groups were given equal volume of normal saline. The function recovery of spinal cord was evaluated by Basso, Beattie, Bresnahan (BBB) scores, and Rivlin inclined plane experiment at postoperative 1, 3, 7, 14, 21 and 28 days. The morphological changes of spinal cord were observed after perfusion at 28 days, expression level of glial fibrillary acidic protein was detected by immunofluorescence, and changes of Nissl body were observed through Nissl staining.
RESULTS AND CONCLUSION: BBB and Rivlin inclined plane scores in each group were significantly decreased postoperatively, and the scores then increased with time; the scores at each time point in the sham group were significantly higher than those in the other groups (P < 0.05); the scores in the gastrodine group were significantly higher than those in the SCI group from day 3 (P < 0.05). At postoperative 28 days, the expression level of glial fibrillary acidic protein was the lowest in the sham group, followed by gastrodine group, and the highest in the SCI group (P < 0.05). In the gastrodine and SCI groups, Nissl bodies were slightly stained partially dissolved, and the account of Nissl bodies in this two groups was less than that in the sham group (P < 0.05), but the gastrodine group had significantly more Nissl bodies than the SCI group (P < 0.05). The spinal cord in the sham group revealed the complete structure; in the SCI group, the structure of spinal cord was in disorder, syringomyelia formatted, and the gray-white matter boundary was obscure; gastrodine group showed smaller syringomyelia and gray-white matter boundary were clear. These results show that gastrodin can inhibit the proliferation of astrocytes after SCI, play neuroprotection, and improve motor function.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Spinal Cord Injuries, Gastrodia, Nerve Regeneration, Tissue Engineering

中图分类号: