中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (23): 4181-4186.doi: 10.3969/j.issn.1673-8225.2012.23.001

• 骨髓干细胞 bone marrow stem cells •    下一篇

膜联蛋白A1在兔骨髓间充质干细胞体外诱导成骨和成脂早期的表达

潘新元1，梁博伟2，赵劲民2，殷国前1   

  1. 广西医科大学第一附属医院，1整形美容外科，2创伤手外科，广西壮族自治区南宁市 530021
  • 收稿日期:2011-10-18 修回日期:2011-11-02 出版日期:2012-06-03 发布日期:2013-11-06
  • 通讯作者: 赵劲民，博士生导师，教授，主任医师，广西医科大学第一附属医院，创伤手外科，广西壮族自治区南宁市 530021 zhaojinmin@126.com
  • 作者简介:潘新元★，男，1984年生，广西壮族自治区隆安县人，壮族，广西医科大学在读硕士，主要从事组织修复方面的研究。 u2coldplay@163.com 梁博伟★，男，1973年生，广西壮族自治区博白县人，汉族，主治医师，2005年中山大学毕业，硕士，主要从事创伤、脊柱外科研究。 LBW5515@163.com
  • 基金资助:

    国家自然科学基金资助项目(30860285)，课题名称：早期激素性股骨头缺血性坏死的蛋白质组学动态分析

Expression of annexin A1 in rabbit bone marrow mesenchymal stem cells during their early differentiation into osteoblasts and adipocytes in vitro

Pan Xin-yuan1, Liang Bo-wei2, Zhao Jin-min2, Yin Guo-qian1   

  1. 1Department of Plastic and Aesthetic Surgery,
    2Department of Trauma and Hand Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China;
  • Received:2011-10-18 Revised:2011-11-02 Online:2012-06-03 Published:2013-11-06
  • Contact: Zhao Jin-min, Doctoral supervisor, Professor, Chief physician, Department of Trauma and Hand Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China zhaojinmin@126.com
  • About author:Pan Xin-yuan★, Studying for master’s degree, Department of Plastic and Aesthetic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China Liang Bo-wei★, Master, Attending physician, Department of Trauma and Hand Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China LBW5515@163.com u2coldplay@163.com

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328

被引次数

1

摘要：

背景：骨髓间充质干细胞分化过程中膜联蛋白A1表达变化的研究报道各有不同看法。
目的：分析兔骨髓间充质干细胞在体外诱导成骨和成脂过程中膜联蛋白 A1基因的表达变化。
方法：应用全骨髓贴壁法分离培养骨髓间充质干细胞，分别加入含成骨诱导剂、成脂诱导剂及不添加任何诱导剂的培养基进行培养。
结果与结论：膜联蛋白A1基因在成骨诱导过程中与未诱导细胞相比有明显下调趋势，差异有显著性意义(P < 0.01)，而在成脂诱导剂中则为上升(P < 0.01)。成骨诱导剂对细胞生长存在抑制作用并增加细胞凋亡(P < 0.01)，而成脂诱导剂对细胞生长与凋亡作用较小(P > 0.05)。因此排除了诱导剂对细胞的作用之后，推测膜联蛋白A1基因可能与骨髓间充质干细胞体外向脂肪细胞分化存在一定关系，但尚不能肯定其在成骨分化中的作用。

关键词: 膜联蛋白A1, 骨髓间充质干细胞, 体外诱导成骨、成脂, 荧光定量RT-PCR, 干细胞

Abstract:

BACKGROUND: Studies have reported different views on the expression of annexin A1 (ANX A1) in bone marrow mesenchymal stem cells (BMSCs) during their differentiation.
OBJECTIVE: To investigate the expression of ANX A1 in rabbit BMSCs during their differentiation into osteoblasts and adipocytes in vitro.
METHODS: BMSCs were isolated by whole bone marrow adherence method, and then the cells were cultured in media with osteogenic inducer, adipogenic inducer and non-inducer respectively.
RESULTS AND CONCLUSION: The expression of ANX A1 mRNA was obviously decreased during the cell differentiation into osteoblasts compared with those without differentiation (P < 0.01). In contrast, the expression of ANX A1 mRNA was obviously increased in BMSCs cultured in medium with adipogenic inducer (P < 0.01). Osteogenic inducer could inhibit the growth of BMSCs to achicve an increase in apoptosis (P < 0.01), while the adipogenic inducer had little effects on cell growth and apoptosis (P > 0.05). ANX A1 may play an important role in regulation of BMSCs differentiation into adipocytes in vitro without the role of inducer. However, the association with osteogenic differentiation is uncertain.

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