中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (22): 4092-4095.doi: 10.3969/j.issn.1673-8225.2012.22.024

• 骨质疏松基础实验 basic experiments of osteoporosis • 上一篇    下一篇

仙灵骨葆对尾悬吊拟失重大鼠骨量丢失的抑制

皮俊杰1，吕志伟2，闫志荣1，荣建民3   

  1. 1唐山市开滦医院分院，河北省唐山市  063000；2河北联合大学，河北省唐山市063000；3唐山市开滦医院，河北省唐山市  063000
  • 收稿日期:2011-09-24 修回日期:2011-10-31 出版日期:2012-05-27 发布日期:2012-05-27
  • 通讯作者: 吕志伟，讲师，河北联合大学解剖教研室，河北省唐山市063000 ncmclzw@126.com
  • 作者简介:皮俊杰，男，1973年生，河北省安平县人，汉族，1996年华北煤炭医学院毕业，主治医师，主要从事外科疾病的基础与临床研究。pjj123654@sohu.com tfm9911316202@163.com

Inhibition of Xianlinggubao on bone loss in tail-suspended rats

Pi Jun-jie1, Lü Zhi-wei2,Yan Zhi-rong1, Rong Jian-min3   

  1. 1Branch of Kailuan Hospital, Tangshan  063000, Hebei Province, China; 2Hebei United University, Tangshan  063000, Hebei Province, China; 3Kailuan Hospital, Tangshan  063000, Hebei Province, China
  • Received:2011-09-24 Revised:2011-10-31 Online:2012-05-27 Published:2012-05-27
  • Contact: Lü Zhi-wei, Lecturer, Hebei United University, Tangshan 063000, Hebei Province, China ncmclzw@126.com
  • About author:Pi Jun-jie, Attending physician, Branch of Kailuan Hospital, Tangshan 063000, Hebei Province, China pjj123654@sohu.com tfm9911316202@163.com

PDF

351

被引次数

4

摘要：

背景：以淫羊藿苷为主要成分的中药制剂仙灵骨葆具有促进骨形成的作用。
目的：观察仙灵骨葆对尾悬吊拟失重大鼠骨量丢失的抑制作用及机制。
方法：将SD大鼠随机分为正常对照组、拟失重组、仙灵骨葆组，后两组大鼠采用尾悬吊法模拟失重，仙灵骨葆组悬吊同时灌胃给予仙灵骨葆250 mg/(kg•d)，拟失重组悬吊同时灌胃给予双蒸水，4周后取右侧股骨行骨密度、三点弯曲生物力学及骨组织形态计量学检测。
结果与结论：正常对照组骨密度、胫骨近端骨小梁相对体积、骨小梁厚度、骨小梁数量、生物力学最大载荷显著高于拟失重组、仙灵骨葆组(P < 0.05)。仙灵骨葆组胫骨远端1/4骨密度、胫骨近端骨小梁相对体积显著高于拟失重组(P < 0.05)，生物力学最大载荷也高于拟失重组，但差异无显著性意义。说明仙灵骨葆灌胃干预可促进松质骨的骨形成，部分阻止拟失重大鼠骨量的丢失。
 

关键词: 拟失重, 仙灵骨葆, 骨密度, 骨组织形态计量学, 生物力学

Abstract:

BACKGROUND: Chinese herbal preparation Xianlinggubao whose main ingredient is icariin can promote bone formation.
OBJECTIVE: To investigate the effect and related mechanism of Xianlinggubao on bone loss in tail-suspended rats.
METHODS: Sprague-Dawley rats were divided into 3 groups: control group (A), simulated weightlessness group (B) and Xianlinggubao group (C). Rats in the group B and group C underwent tail-suspension, and in the group C was administered Xianlinggubao by gavage with dosage of 250 mg/(kg•d) at the same time while in the group B was administered double distilled water as control. Bone mineral density (BMD), biomechanical three-point bending and bone morphology of the right femurs were detected after 4 weeks removing the right femurs.
RESULTS AND CONCLUSION: BMD, trabecular bone volume, bone trabecular thickness, trabecular number and biomechanics maximum load in the group A were markedly higher than those in groups B and C (P < 0.05), BMD of tibia distal 1/4, trabecular bone volume of proximal tibia in group C were higher as compared to group B (P < 0.05). Biomechanics maximum load in the group C was also higher than that of the group B, but there was no significant difference. Xianlinggubao inhibition by gavage can promote bone formation and partially prevent bone loss in tail-suspended rats.

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