中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (17): 3078-3081.doi: 10.3969/j.issn.1673-8225.2012.17.010

• 骨科植入物 orthopedic implant • 上一篇    下一篇

激光共聚焦显微镜观察钛板和死骨表面细菌生物膜的形成能力*

张志宏，孙效棠，冯安平，王万明   

  1. 解放军南京军区福州总医院骨二科，福建省福州市  350025
  • 收稿日期:2012-01-04 修回日期:2012-03-02 出版日期:2012-04-22 发布日期:2012-04-22
  • 通讯作者: 孙效棠，博士，主治医师，主要从事创伤和脊柱外科，福州总医院骨二科，福建省福州市 350025
  • 作者简介:张志宏，男，1966年生，辽宁省抚顺市人，汉族，1990年解放军第三军医大学毕业，副主任医师，主要从事创伤和脊柱外科研究。
  • 基金资助:

    福建省自然科学基金资助(2009J05082)。

Formation capability of bacterial biofilm on titanium plate versus necrotic bone

Zhang Zhi-hong, Sun Xiao-tang, Feng An-ping, Wang Wan-ming   

  1. Second Department of Orthopedics and Traumatology, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA, Fuzhou 350025, Fujian Province, China
  • Received:2012-01-04 Revised:2012-03-02 Online:2012-04-22 Published:2012-04-22
  • Contact: Sun Xiao-tang, Doctor, Attending physician, Second Department of Orthopedics and Traumatology, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA, Fuzhou 350025, Fujian Province, China xiaotangsun@hotmail.com
  • About author:Zhang Zhi-hong, Associate chief physician, Second Department of Orthopedics and Traumatology, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA, Fuzhou 350025, Fujian Province, China zhihongzhang85@yahoo.com.cn
  • Supported by:

     the Natural Science Foundation of Fujian Province, No. 2009J05082*

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被引次数

1

摘要：

背景：细菌在金属植入物和死骨表面附着并形成的生物膜是造成骨骼肌肉系统的慢性感染的根本原因。
目的：观察并比较细菌在钛板和死骨表面形成细菌生物膜的能力。
方法：用改良的基质培养法制备细菌生物膜模型，将10块钛板圆柱和10片死骨随机配对，每一对放置在同一个培养瓶中用其菌液浸泡淹没，在同一个培养环境中培养。用荧光染料对各组细菌生物膜进行染色，激光共聚焦显微镜下观察并采集图像。
结果与结论：与死骨相比，钛板表面的细菌生物膜厚度较小(P < 0.05)，其细菌生物膜中层和深层的活菌率较高(P < 0.05)。说明细菌在死骨形表面成细菌生物膜的能力强于钛板。
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关键词: 细菌生物膜, 钛板, 死骨, 激光共聚焦显微镜, 硬组织植入物

Abstract:

BACKGROUND: Bacteria attachment and biofilm formation at the surface of metal implants and sequestrum are major reasons for chronic infection of musculoskeletal system.
OBJECTIVE: To characterize and compare the formation capability of bacterial biofilm on titanium plate and necrotic bone.
METHODS: The model of bacterial biofilm was developed with modified stroma culture approach. Ten titanium plate cylinders and ten pieces of necrotic bone were randomly matched. Each pair was placed in one culture flask and immersed in bacteria solution. The biofilm of each group was stained with fluorochrome, observed and photographed using confocal laser scanning microscope.
RESULTS AND CONCLUSION: Compared with the necrotic bone, the thickness of bacterial biofilm on the titanium plate was less
(P < 0.05) and the percentage of live bacteria in the medium layer and the bottom layer was higher (P < 0.05). Results indicate that the bacterial biofilm tends to more easily develop on the surface of necrotic bone than on the surface of titanium plate.

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