中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (16): 2939-2942.doi: 10.3969/j.issn.1673-8225.2012.16.021

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

外源性单磷酸鸟苷环二聚体抑制变形链球菌生物膜的形成能力*☆

闫文娟   

  1. 南方医科大学南方医院口腔科，南方医科大学口腔医学院，广东省广州市  510515
  • 收稿日期:2011-10-01 修回日期:2012-01-13 出版日期:2012-04-15 发布日期:2012-04-15
  • 作者简介:闫文娟☆，女，1973年生，山东省青州市人，汉族，2009年解放军第四军医大学毕业，博士，主治医师，主要从事龋病的预防研究。ywj918@ sohu.com
  • 基金资助:

    国家自然科学基金资助项目(81100747)。

Inhibitory effect of exogenous bis-(3'-5')-cyclic dimeric guanosine monophosphate on the biofilm formation of Streptococcus mutans

Yan Wen-juan   

  1. Department of Stomatology, Nanfang Hospital of Southern Medical University, Guangzhou   510515, Guangdong Province, China
  • Received:2011-10-01 Revised:2012-01-13 Online:2012-04-15 Published:2012-04-15
  • About author:Yan Wen-juan☆, Doctor, Attending physician, Department of Stomatology, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China ywj918@sohu.com
  • Supported by:

    the National Natural Science Foundation of China, No. 81100747*

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摘要：

背景：有研究发现外源性的单磷酸鸟苷环二聚体(bis-(3'-5')-cyclic dimeric guanosinemonophosphate，c-di-GMP)能够抑制金黄色葡萄球菌生物膜的形成，且作用呈剂量依赖性。
目的：观察外源性c-di-GMP对变形链球菌生物膜形成能力的影响。
方法：将不同浓度(0，2，20，200，400 µmol/L)外源性c-di-GMP作用于变形链球菌生物膜48 h，使用酶标仪测定吸光度值，观测生物膜形成量的改变，以生理盐水作为阴性对照。同时在离体牙的新鲜釉质片上形成变形链球菌生物膜，以      200 μmol/L的c-di-GMP与生理盐水分别作用于生物膜48 h，扫描电镜观察结构的改变。
结果与结论：与阴性对照组比较，c-di-GMP明显抑制了变形链球菌生物膜的形成，而且这种抑制成剂量依赖关系，当c-di-GMP浓度为200 µmol/L时，变形链球菌生物膜的形成能力下降了65%左右，达到400 µmol/L时，生物膜的形成能力几乎被完全抑制(P < 0.05)。扫描电镜结果显示，c-di-GMP处理组细菌排列无明显规律，细胞外基质减少。表明c-di-GMP可以抑制变形链球菌的生物膜形成能力。
 

关键词: 单磷酸鸟苷环二聚体(c-di-GMP), 变形链球菌, 生物膜, 金黄色葡萄球菌, 龋齿, 外源性

Abstract:

BACKGROUND: Studies have found that the exogenous bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) can inhibit biofilm formation of staphylococcus aureus in a dose-dependent manner.
OBJECTIVE: To investigate the effect of c-di-GMP on biofilm formation of Streptococcus mutans.
METHODS: Different concentrations of exogenous c-di-GMP (0, 2, 20, 200, 400 µmol/L) were acted on Streptococcus mutans biofilms for 48 hours. Absorbance value was measured by using microplate reader. Changes of biofilms formation were observed. Similar volumes of normal saline were added as negative control. At the meantime, Streptococcus mutans biofilms formed in vitro on fresh enamel discs of extraction tooth were also acted on with the concentration of 200 μmol/L c-di-GMP and normal saline for   48 hours respectively and observed under scanning electron microscope.
RESULTS AND CONCLUSION: The biofilm formation of the Streptococcus mutans was significantly inhibited in the presence of c-di-GMP as compared with the negative control group. When the concentration of c-di-GMP was 200 μmol/L, the biofilm formation ability of the Streptococcus mutans decreased by 65% and when the concentration was 400 μmol/L, the biofilm formation ability was almost inhibited (P < 0.05). Results of scanning electron microscope showed that Streptococcus mutans were not regularly and the extra cellular polysaccharide was reduced with the pulsing of c-di-GMP. It is showed that c-di-GMP can inhibit the biofilm formation ability of Streptococcus mutans.

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