中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (15): 2801-2804.doi: 10.3969/j.issn.1673-8225.2012.15.031

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

锶矿泉水对人肾小管上皮细胞增殖及ATP酶活性的影响*

蔺  艳1，盘强文1，冯志强1，李著华1，赵成涛2，冉  兵1   

  1. 1泸州医学院生理教研室，四川省泸州市646000；   2泸州云峰集团，四川省泸州市  64600
  • 收稿日期:2012-01-12 修回日期:2012-03-05 出版日期:2012-04-08 发布日期:2012-04-08
  • 通讯作者: 冉兵，副教授，硕士生导师，泸州医学院生理教研室，四川省泸州市 646000 rbfx@163.com
  • 作者简介:蔺艳，女，1973年生，汉族，重庆市人，1997年泸州医学院毕业，讲师，主要从事肾功能保护的研究。 ymndemail@sohu.com
  • 基金资助:

    四川省卫生厅项目(090188)。

Effects of strontium mineral water on the proliferation and adenosine-triphosphatase activity of human renal tubular epithelial cells

Lin Yan1, Pan Qiang-wen1, Feng Zhi-qiang1, Li Zhu-hua1, Zhao Cheng-tao2, Ran Bing1   

  1. 1Teaching and Research Room of Physiology, Luzhou Medical College, Luzhou  646000, Sichuan Province, China; 2Luzhou Yunfeng Group, Luzhou  646000, Sichuan Province, China
  • Received:2012-01-12 Revised:2012-03-05 Online:2012-04-08 Published:2012-04-08
  • Contact: author: Ran Bing, Associate professor, Master’s supervisor, Teaching and Research Room of Physiology, Luzhou Medical College, Luzhou 646000, Sichuan Province, China rbfx@163.com
  • About author:Lin Yan, Lecturer, Teaching and Research Room of Physiology, Luzhou Medical College, Luzhou 646000, Sichuan Province, China ymndemail@sohu.com
  • Supported by:

    Project of Department of Health of Sichuan Province, No. 090188*

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摘要：

背景：一定浓度的锶对人体健康有益，锶对人体多种细胞的增殖和酶活性有影响。
目的：用不同浓度的锶矿泉水配制的培养基在体外培养人肾小管上皮细胞，观察细胞增殖情况及Na+-K+-ATP酶的活性。
方法：体外培养人肾小管上皮细胞，用普通DMEM液体培养基(高糖)培养24 h后，分别用含锶质量浓度2，4，6，8 mg/L的矿泉水和双蒸水的条件培养基继续培养，分别于48，72，96和110 h行MTT法观察HK-2细胞的增殖情况。培养110 h的细胞以比色法测无机磷的量来判断Na+-K+-ATP酶的活性。
结果与结论：MTT结果表明：质量浓度2 mg/L锶矿泉水对HK-2细胞增殖无影响；细胞培养至110 h，双蒸水组出现生长抑制时，质量浓度4，6，8 mg/L锶矿泉水组却依然能保持旺盛的增殖状态(P < 0.05)，生长周期延长，尤以质量浓度4 mg/L锶矿泉水组的效果最突出(P < 0.05)。Na+-K+-ATP酶活性测试结果表明：与双蒸水组比较，锶矿泉水组Na+-K+-ATP酶活性更高(P < 0.05)，其中质量浓度6 mg/L锶矿泉水组的酶活性最强(P < 0.05)。结果证实，锶矿泉水可延长肾小管细胞的增殖周期，可促进肾小管细胞的转运功能。
关键词：锶；肾小管细胞；增殖；ATP酶；矿泉水；浓度；组织工程
doi:10.3969/j.issn.1673-8225.2012.15.031

关键词: 锶, 肾小管细胞, 增殖, ATP酶, 矿泉水, 浓度, 组织工程

Abstract:

BACKGROUND: A certain concentration of strontium is beneficial for the human body. Strontium can affect the proliferation and enzyme activity of a variety of cells in the human body.
OBJECTIVE: To investigate the effects of strontium mineral waters with different concentrations on the proliferation and Na+-K+-adenosine-triphosphatase (Na+-K+-ATPase) activity of human renal tubular epithelial cells (HK-2 cells).
METHODS: Human renal tubular epithelial cells were cultured in vitro. After cultured in a normal Dulbecco’s Modified Eagle’s Medium (high glucose) for 24 hours, the cells were cultured in the conditioned medium containing 2, 4, 6, 8 mg/L strontium mineral waters and double distilled water. The proliferation of HK-2 cells was observed by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay at hours 48, 72, 96 and 110. The Na+-K+-ATPase activity of the cells cultured for 110 hours was judged by measuring the amount of inorganic phosphorus.
RESULTS AND CONCLUSION: The results of MTT showed that 2 mg/L strontium mineral water could not affect the proliferation of HK-2 cells. When the cells were cultured for 110 hours, the cells in the double distilled water group were found growth inhibition, while the proliferation of the cells in the 6, 8, 10 mg/L strontium mineral water groups could still keep vigorous (P < 0.05). Moreover, the cell growth cycle was prolonged in the 6, 8, 10 mg/L strontium mineral water groups, especially in the 4 mg/L strontium mineral water group (P < 0.05). Compared with the double distilled water group, the Na+-K+-ATPase activity of strontium mineral waters groups was higher in the strontium mineral water groups (P < 0.05), especially in the 6 mg/L strontium mineral water group (P < 0.05). These findings suggest that the strontium mineral water with higher concentration can prolong proliferation cycle of renal tubular cells and promote the transport function of renal tubular cells.

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