中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (12): 2158-2162.doi: 10.3969/j.issn.1673-8225.2012.12.016

• 膜生物材料 membrane biomaterials • 上一篇    下一篇

肺癌患者中心静脉导管相关表皮葡萄球菌icaA、icaD表达及转化生长因子β1与 生物膜的关系**☆

雷玉洁1，黄云超1，杨  丽2，郭凤丽2，徐玉善3，叶联华1，陈小波1，赵光强1，贺  猛1   

  1. 昆明医学院第三附属医院(云南省肿瘤医院)， 1胸心外科，2检验科，云南省昆明市  650118; 3昆明医学院第一附属医院内分泌与代谢病科，云南省昆明市  650032
  • 收稿日期:2011-07-13 修回日期:2011-08-15 出版日期:2012-03-18 发布日期:2012-03-18
  • 通讯作者: 黄云超，教授，博士生导师，昆明医学院第三附属医院胸心外科，云南省昆明市 650118
  • 作者简介:雷玉洁☆，女，1977年生，云南省丽江市人，昆明医学院在读博士，讲师，主要从事胸心外科生物材料相关研究。
  • 基金资助:

    国家自然科学基金项目(30872555); 云南省科技厅社会发展科技计划应用基础研究项目(2009ZC116M)。

Correlation between central venous catheter-related staphylococcus epidermidis icaA, icaD, transforming growth factor beta 1 and biofilms in lung cancer patients**☆

Lei Yu-jie1, Huang Yun-chao1, Yang Li2, Guo Feng-li2, Xu Yu-shan3, Ye Lian-hua1, Chen Xiao-bo1,  Zhao Guang-qiang1, He Meng1   

  1. 1Department of Cardiothoracic Surgery, 2Department of Clinical Laboratory, Third Affiliated Hospital of Kunming Medical University (Yunnan Tumor Hospital), Kunming  650118, Yunnan Province, China; 3Department of Endocrinology, First Affiliated Hospital of Kunming Medical University, Kunming  650032, Yunnan Province, China
  • Received:2011-07-13 Revised:2011-08-15 Online:2012-03-18 Published:2012-03-18
  • Contact: author: Huang Yun-chao, Professor, Doctoral supervisor, Department of Cardiothoracic Surgery, Third Affiliated Hospital of Kunming Medical University (Yunnan Tumor Hospital), Kunming 650118, Yunnan Province, China huangych2001@ yahoo.com.cn
  • About author:Lei Yu-jie☆, Studying for doctorate, Lecturer, Department of Cardiothoracic Surgery, Third Affiliated Hospital of Kunming Medical University (Yunnan Tumor Hospital), Kunming 650118, Yunnan Province, China leiyujie952499@ yahoo.com.cn
  • Supported by:

     the National Natural Science Foundation of China, No. 30872555*; the Application Basis of Science and Technology Development Plan of Yunnan Province, No. 2009ZC116M*

PDF

407

摘要：

背景：研究证实，以生物材料为中心的感染细菌临床株致病力与其在中心静脉导管材料表面形成细菌生物膜的能力呈正相关。
目的：分析肺癌患者中心静脉导管相关表皮葡萄球菌icaA、icaD mRNA表达及外周血转化生长因子β1水平与细菌生物膜形成的关系。
方法：种属鉴定相关性血流感染肺癌患者表皮葡萄球菌类型后行细菌基因组 DNA 抽提，PCR法检测生物膜形成相关基因icaA、icaD mRNA表达及生物膜表型。酶联免疫吸附试验检测相关性血流感染与未感染肺癌患者血清转化生长因子β1水平。
结果与结论：相关性血流感染肺癌患者表皮葡萄球菌操纵子icaA、icaD基因表达与生物膜形成呈正相关(P < 0.01)，且表皮葡萄球菌生物膜阳性患者外周血转化生长因子β1水平较无相关性血流感染肺癌患者高(P < 0.05)。表明置入中心静脉插管引起表皮葡萄球菌感染icaA、icaD基因表达阳性肺癌患者较易形成细菌生物膜，外周血高水平转化生长因子β1对细菌生物膜形成有积极作用。

关键词: 肺癌, 留置导管, 导管材料,  , ica基因, 转化生长因子&beta, 1

Abstract:

BACKGROUND: It is confirmed that pathogenicity of biomaterials-centered infection is positive correlated to bacterial biofilm formation of Staphylococcus epidermidis on the surface of catheter-related materials. 
OBJECTIVE: To analyze the relations between expressions of central venous catheter-related Staphylococcus epidermidis icaA, icaD, transforming growth factor β1 (TGF-β1) and formation of bacterial biofilm.
METHODS: The type of Staphylococcus epidermidis in lung cancer cases with catheter-related bloodstream infections (CRBSI) was indentified, followed by bacterial genomic DNA extraction. The expression of biofilm formation-related genes icaA, icaD mRNA and phenotype of biofilm were detected by PCR. The serum TGF-β1 levels in cases with or without CRBSI were measured by enzyme-linked immunosorbent assay.
RESULTS AND CONCLUSION: The expression of Staphylococcus epidermidis operon icaA and icaD gene was positive correlated to biofilm formation in lung cancer cases with CRBSI (P < 0.01); particularly, the TGF-β1 levels in CRBSI cases were greater than that of non-CRBSI cases (P < 0.05). The results demonstrated that, central venous catheter infection causes positive Staphylococcus epidermidis icaA and icaD gene expressions in lung cancer cases and is prone to form biofilm, high level of peripheral TGF-β1 may play a positive role in bacterial biofilm formation.

中图分类号: