中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (10): 1871-1874.doi: 10.3969/j.issn.1673-8225.2012.10.035

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

流体切应力与内皮祖细胞铜锌超氧化物歧化酶基因表达及活性*****☆

杨  震1，赖光华1，夏文豪1，罗初凡2，王洁梅1，陈  龙1，廖新学1，靳亚飞1，陶  军1   

  1. 中山大学附属第一医院，1高血压血管病科，2心血管介入病区，广东省广州市 510080
  • 收稿日期:2011-09-05 修回日期:2011-11-01 出版日期:2012-03-04 发布日期:2012-03-04
  • 通讯作者: 陶军，博士，教授，博士生导师，中山大学附属第一医院高血压血管病科，广东省广州市 510080 taojungz@yahoo.com
  • 作者简介:杨震☆，男，1978年生，湖南省衡阳市人，汉族，2007年中山大学毕业，博士，主治医师，主要从事心血管内科方面的研究。yangzhen107107@yahoo.com.cn
  • 基金资助:

    国家自然科学基金资助项目(30770895，   u0732002, 30800215)；广东省自然科学基金博士启动项目(8451008901000793)；广州市科技计划(2007Z3-E0241)。

Effect of shear stress on the copper-zinc superoxide dismutase gene expression and activity in human endothelial progenitor cells 

Yang Zhen1, Lai Guang-hua1, Xia Wen-hao1, Luo Chu-fan2, Wang Jie-mei1, Chen Long1, Liao Xin-xue1, Jin Ya-fei1, Tao Jun1   

  1. 1Department of Hypertension & Vascular Disease; 2Department of Cardiac Intervention, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China
  • Received:2011-09-05 Revised:2011-11-01 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Tao Jun, M.D., Professor, Doctoral supervisor, Department of Hypertension & Vascular Disease, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China taojungz@yahoo.com
  • About author:Yang Zhen☆, M.D., Attending physician, Department of Hypertension & Vascular Disease, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China yangzhen107107@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30770895*, u0732002*, 30800215*; Doctoral Launching Project of Natural Science Foundation of Guangdong Province, No. 84510089010007 93*; Science and Technology Development Program of Guangzhou Province, No. 2007Z3-E0241*

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摘要：

背景：流体切应力是调控内皮祖细胞的重要非药理学手段，但目前其对内皮祖细胞抗氧化功能的作用尚不清楚。
目的：观察不同切应力对内皮祖细胞铜锌超氧化物歧化酶(Cu/Zn-superoxide dismutase，Cu/Zn-SOD)基因表达和活性的影响，以探讨流体切应力对内皮祖细胞抗氧化功能的调节作用。
方法：健康成人外周血的单个核细胞诱导分化为内皮祖细胞，分为静态组、低切应力组、中切应力组和高切应力组4组进行观察。
结果与结论：外周血单个核细胞分化成为内皮祖细胞，倒置荧光显微镜下呈典形的“纺锤样”梭形细胞，ac-LDL吞噬及lectin抗体荧光标记双阳性，FLK-1和VWF免疫荧光抗体染色均为阳性。各切应力组内皮祖细胞CuZn-SOD活性均高于静态组，并且切应力越大，内皮祖细胞Cu/Zn-SOD分泌水平越高。荧光定量RT-PCR表明，切应力处理能上调内皮祖细胞CuZn-SOD mRNA表达，并且切应力水平越高，CuZn-SOD mRNA表达越强。说明切应力能上调内皮祖细胞Cu/Zn-SOD基因表达，增强内皮祖细胞Cu/Zn-SOD活性，提高内皮祖细胞的抗氧化活性。因此，在生理范围内增加体外切应力，能上调内皮祖细胞的功能活性。
关键词：内皮祖细胞；切应力；超氧化物歧化酶；内皮；基因表达
doi:10.3969/j.issn.1673-8225.2012.10.035

关键词: 关键词：内皮祖细胞, 切应力, 超氧化物歧化酶, 内皮, 基因表达

Abstract:

BACKGROUND: Shear stress is an important non-pharmacological method to regulated endothelial progenitor cells (EPCs). However, the effect of shear stress on the anti-oxidative activity of EPCs is not clear.
OBJECTIVE: To observe the effect of shear stress on the copper-zinc superoxide dismutase (Cu/Zn-SOD) gene expression and activity in EPCs, in order to investigate the regulatory effects of shear stress on the EPCs function.
METHODS: The peripheral blood mononuclear cells of healthy adult were inducted into EPCs. Then, they were divided into four different experimental groups which included stationary group, low-flow shear stress group (0.05 mN /cm2), medium-flow shear stress group (0.15 mN/cm2) and high-flow shear stress group (0.25 mN/cm2).
RESULTS AND CONCLUSION: The peripheral blood mononuclear cells were differentiated into EPCs. They presented typical “spindle-shaped” appearance, and were positively labeled by acetylated-LDL, lectin, FLK-1 and VWF. The Cu/Zn-SOD activity of human EPCs in shear stress treatment groups was higher than that in stationary group. The higher the shear stress, the higher the Cu/Zn-SOD activity of human EPCs. Quantitative real-time PCR indicated that shear stress could increase the CuZn-SOD mRNA expression of EPCs, the higher the shear stress, the stronger the CuZn-SOD mRNA expression of EPCs. In vitro shear stress could upregulate the gene expression of Cu/Zn-SOD in EPCs, increase the Cu/Zn-SOD activity in human EPCs, which contributes to enhance the anti-oxidative activity of EPCs. Therefore, enhanced shear stress within the physiological range could regulate the function of EPCs, which can act as an important strategy of enhancing their therapeutic approach for vascular endothelial injury.

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