中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (10): 1833-1836.doi: 10.3969/j.issn.1673-8225.2012.10.027

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

骨髓间充质干细胞移植影响慢性脑缺血大鼠认知功能及海马区Nogo-A、NgR的表达*★

王燕琳，闫  禹，宋  波，张惠莉，龚光明，陈  思，方  慧，许予明   

  1. 郑州大学第一附属医院神经内三科，河南省郑州市 450052
  • 收稿日期:2012-02-03 修回日期:2012-02-13 出版日期:2012-03-04 发布日期:2012-03-04
  • 通讯作者: 许予明，博士，主任，教授，博士生导师，郑州大学第一附属医院神经内三科，河南省郑州市 450052 xuyuming@zzu.edu.cn
  • 作者简介:王燕琳★，女，1986年生，郑州大学在读硕士，主要从事脑血管病的基础与临床研究。410215135@qq.com
  • 基金资助:

    河南省重大公益科研项目(091100910800)。

Transplantation of bone marrow mesenchymal stem cells improves cognitive function of rats with chronic cerebral ischemia and decreases Nogo-A and NgR protein expression in the hippocampus   

Wang Yan-ling, Yan Yu, Song Bo, Zhang Hui-li, Gong Guang-ming, Chen Si, Fang Hui, Xu Yu-ming   

  1. Third Department of Neurology, First Affiliated Hospital of Zhengzhou University, Zhengzhou   450052, Henan Province, China
  • Received:2012-02-03 Revised:2012-02-13 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Xu Yu-ming, M.D., Professor, Doctoral supervisor, Third Department of Neurology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China xuyuming@ zzu.edu.cn.
  • About author:Wang Yan-lin★, Studying for master’s degree, Third Department of Neurology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China 410215135@qq.com
  • Supported by:

     the Major Program of Public Science Research of Henan Province, No. 091100910800*

PDF

358

被引次数

4

摘要：

背景：骨髓间充质干细胞移植可降低脊髓损伤及脑梗死大鼠的Nogo-A及NgR的表达。
目的：观察骨髓间充质干细胞移植对慢性脑缺血大鼠认知功能及海马区Nogo-A及NgR蛋白的表达。
方法：将30只SD大鼠随机等分为假手术组、模型组及骨髓间充质干细胞组，后2组采用双侧颈总动脉永久结扎法建立大鼠慢性脑缺血模型，骨髓间充质干细胞组在建模7 d后尾静脉注射移植用ficoll密度梯度离心法分离培养大鼠骨髓间充质干细胞1×107个。
结果与结论：与假手术组比较，模型组大鼠平均逃避潜伏期明显延长(P < 0.01)，穿过原平台位置次数明显减少(P < 0.01)，海马中Nogo-A与NgR蛋白表达明显增加(P < 0.05)；而与模型组比较，骨髓间充质干细胞组大鼠平均逃避潜伏期明显缩短(P < 0.01)，穿过原平台位置次数明显增加(P < 0.05)，海马组织中Nogo-A及NgR表达明显减少(P < 0.05)。说明骨髓间充质干细胞移植具有保护慢性脑缺血大鼠空间学习与记忆能力的作用，其作用可能与降低Nogo-A及NgR蛋白表达有关。
关键词：骨髓间充质干细胞；细胞移植；慢性脑缺血；Nogo-A；NgR；认知
doi:10.3969/j.issn.1673-8225.2012.10.027

关键词: 骨髓间充质干细胞, 细胞移植, 慢性脑缺血, Nogo-A, NgR, 认知

Abstract:

BACKGROUND: Transplantation of bone marrow mesenchymal stem cells can decrease Nogo-A and NgR expression in rats with spinal cord injury and cerebral infarction.
OBJECTIVE: To investigate the effects of transplantation of bone marrow mesenchymal stem cells on cognitive function of rats with chronic cerebral ischemia and Nogo-A and NgR protein expression in the hippocampus.
METHODS: Thirty Sprague-Dawley rats were randomly divided into sham-surgery, model and bone marrow mesenchymal stem cells groups. Rats in the latter two groups were established into model of chronic cerebral ischemia by permanent ligation of bilateral common carotid artery. In the bone marrow mesenchymal stem cells group, at 7 days after chronic cerebral ischemia induction, bone marrow mesenchymal stem cells (1×107) isolated by ficoll density gradient centrifugation were transfused into rats via the tail vein.
RESULTS AND CONCLUSION: Compared with sham-surgery group, the average escape latency was significantly shortened (P < 0.01), the number that rats passed through the platform was significantly increased (P < 0.05), and Nogo-A and NgR expression in the hippocampal tissue was significantly decreased (P < 0.05) in the bone marrow mesenchymal stem cells group. These findings suggest that transplantation of bone marrow mesenchymal stem cells protects spatial learning and memory abilities of rats with chronic cerebral ischemia. This effect is possibly related to decreased Nogo-A and NgR protein expression.

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