中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (10): 1729-1732.doi: 10.3969/j.issn.1673-8225.2012.10.005

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

心肌组织裂解液诱导骨髓间充质干细胞向心肌组织样结构的分化***★

赵文婧，李  琴，吴  卓，陈维平   

  1. 广西医科大学组织学与胚胎学教研室，广西壮族自治区南宁市 530022
  • 收稿日期:2012-01-04 修回日期:2012-01-19 出版日期:2012-03-04 发布日期:2012-03-04
  • 通讯作者: 陈维平，广西医科大学组织学与胚胎学教研室，广西壮族自治区南宁市 530022 chwp8852@126.com
  • 作者简介:赵文婧★，女，1983年生，湖南省邵东市人，汉族，2012年广西医科大学毕业，硕士，主要从事干细胞应用与基础方面的研究。 182801861@qq.com
  • 基金资助:

    广西科技厅资助项目(桂科青0229009)，人胚胎干细胞分离、克隆与分化研究；广西科技厅资助项目(桂科攻 0592007-1G)，人胚胎干细胞库建立与应用研究；广西科技厅资助项目(桂科自0832157)，胰腺干细胞的基础研究与应用资源的建立 。

Application of myocardial tissue lysates on the differentiation of bone marrow mesenchymal stem cells into cardiac tissue-like structure 

Zhao Wen-jing, Li Qin, Wu Zhuo, Chen Wei-ping   

  1. Department of Histology and Embryology, Guangxi Medical University, Nanning  530022, Guangxi Zhuang Autonomous Region, China
  • Received:2012-01-04 Revised:2012-01-19 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Chen Wei-ping, Department of Histology and Embryology, Guangxi Medical University, Nanning 530022, Guangxi Zhuang Autonomous Region, China chwp8852@126.com
  • About author:Zhao Wen-jing★, Master, Department of Histology and Embryology, Guangxi Medical University, Nanning 530022, Guangxi Zhuang Autonomous Region, China 182801861@qq.com
  • Supported by:

    Fund of Guangxi Science and Technology Agency, No.Guike qing0229009*, Guike gong0592007-1G*, Guikezi0832157*

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摘要：

背景：体内实验提示骨髓间充质干细胞所处的微环境对其分化的方向有着重要的影响。
目的：体外诱导骨髓间充质干细胞分化形成心肌组织样结构。
方法：选用生长良好的第3代骨髓间充质干细胞，以心肌组织裂解液连续培养4周，倒置显微镜下连续观察细胞形态特征的变化规律，收集诱导后的组织行苏木精-伊红染色，电镜观察其超微结构。采用波形蛋白、Ⅷ因子相关抗原进行免疫组织化学染色。
结果与结论：以心肌组织裂解液连续诱导培养4周。第12天部分“竹节样”细胞融合形成多核肌管样结构，第14天肌管样结构出现有节律性的收缩，搏动频率为90~110次/min；透射电镜下细胞内可见肌节样结构，部分肌细胞具自律性搏动的功能特点；苏木精-伊红染色可见大量肌束样结构。提示心肌组织裂解液可模拟心肌组织微环境，有效促使骨髓间充质干细胞分化为具有典型形态结构特征和相应功能活动的心肌细胞，同时诱导部分骨髓间充质干细胞分化为结缔组织细胞、内皮样细胞，与心肌细胞共同形成心肌组织样结构。显示在特定微环境的适宜条件下，骨髓间充质干细胞具有多向分化、发育形成多种功能上密切相关的细胞，形成组织样结构的趋势。
关键词：心肌组织裂解液；组织样结构；体外诱导；分化；间充质干细胞；心肌
doi:10.3969/j.issn.1673-8225.2012.10.005

关键词: 心肌组织裂解液, 组织样结构, 体外诱导, 分化, 间充质干细胞, 心肌

Abstract:

BACKGROUND: In vivo experiment has shown that the microenvironment of bone marrow mesenchymal stem cells (BMSCs) plays an important role on induced differentiation.
OBJECTIVE: To in vitro induce the BMSCs to differentiate into cardiac tissue-like structure.
METHODS: Changes of morphological characteristics of the third generation of BMSCs that cultured with myocardial tissue lysates for 4 weeks were observed under inverted microscope. The tissue was collected and preformed with hematoxylin-eosin staining, and the ultrstructure of the induced cells was observed by electron microscope. Immunohistochemical staining was preformed using vimentin and Ⅷ factor-related antigen.
RESULTS AND CONCLUSION: Myocardial tissue lysates were cultured for 4 weeks. At the 12th day, some “bamboo-like” cells were fused to form multinucleated myotube-like structure. From the 14th day, rhythmic contraction could be observed with the beating frequency about 90-110/min; sarcomere-like structure of filaments could be seen under transimission electron microscope and some muscle cells had the function of automatic pulsating. Hematoxylin-eosin staining showed a large number of bundle-like structures. Myocardial tissue lysates can be used as simulated cardiac tissue microenvironment inducer to promote the BMSCs to differentiate into myocardial cells with typical morphological features and the corresponding functions, and induce part of the BMSCs to differentiate into connective tissue cells, endothelial like cells, and form cardiac tissue-like structure together with cardiac cells. BMSCs have the multiple differentiation potential, forming a variety of cells with similar functions and forming the tissue-like structure trend in a certain microenvironment.

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