中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (10): 1721-1724.doi: 10.3969/j.issn.1673-8225.2012.10.003

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

17β-雌二醇对大鼠骨髓间充质干细胞向成骨细胞分化的诱导调控*****☆

韩  娜1，寇玉辉2，王天兵2，殷晓峰2，何培英1，张培训2   

  1. 北京大学人民医院，1临床分子生物学研究所，2创伤骨科，北京市 100044
  • 收稿日期:2012-01-11 修回日期:2012-01-31 出版日期:2012-03-04 发布日期:2012-03-04
  • 通讯作者: 张培训，博士，副主任医师，北京大学人民医院创伤骨科，北京市 100044 zhangpeixun@126.com
  • 作者简介:韩娜☆，女，1975年生，山东省淄博市人，汉族，2008年北京大学医学部毕业，博士，助理研究员，主要从事骨折愈合、骨代谢、周围神经损伤方面的研究。hannaqd@yahoo.com.cn
  • 基金资助:

    国家自然科学基金项目(30973066、31040043)，北京市自然科学基金项目(7113170)，教育部博士点新教师基金项目(20090001120096)和北京大学人民医院研究与发展基金项目(RDB2010-30)。

Effects of 17 beta-estradiol on osteogenic differentiation of rat primary cultured bone marrow mesenchymal stem cells

Han Na1, Kou Yu-hui2, Wang Tian-bing2, Yin Xiao-feng2, He Pei-ying1, Zhang Pei-xun2   

  1. 1Institute of Clinical Molecular Biology, 2Department of Orthopedics and Traumatology, Peking University People’s Hospital, Beijing 100044, China
  • Received:2012-01-11 Revised:2012-01-31 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Zhang Pei-xun, Department of Orthopedics and Traumatology, Peking University People’s Hospital, Beijing 100044, China zhangpeixun@126.com
  • About author:Han Na☆, M.D., Research Assistant, Institute of Clinical Molecular Biology, Peking University People's Hospital, Beijing 100044, China hannaqd@yahoo.com.cn
  • Supported by:

     the National Natural Science Foundation of China, No. 30973066*, 31040043*; the Natural Science Foundation of Beijing, No. 7113170*; Doctoral Fund of Ministry of Education of China, No. 20090001120096*; Peking University People’s Hospital Research and Development Funds, No. RDB2010-30*

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摘要：

背景：研究发现骨髓间充质干细胞上存在雌激素受体，雌激素通过调节骨髓间充质干细胞的增殖、分化特性发挥促成骨作用。
目的：观察17β-雌二醇对骨髓间充质干细胞向成骨细胞分化的诱导作用，并探讨其作用机制。
方法：在基础成骨诱导培养基培养的第3代大鼠骨髓间充质干细胞中分别加入0(对照组)，0.001，0.01，0.1 nmol/L 17β-雌二醇干预。Elisa法检测培养的骨髓间充质干细胞分泌Ⅰ型胶原的水平，RT-PCR及Western blotting法分别检测Runx2因子mRNA及蛋白水平。
结果与结论：与对照组比较，在给予0.001，0.01，0.1 nmol/L 17β-雌二醇干预后第5天，骨髓间充质干细胞Ⅰ型胶原表达显著升高(P < 0.05)，第7天仍旧呈高表达(P < 0.05)。同时，在17β-雌二醇干预的第5，7天，Runx2因子mRNA及蛋白表达水平随17β-雌二醇浓度的增加而升高(P < 0.05)，并呈剂量依赖性。表明17β-雌二醇可诱导骨髓间充质干细胞向成骨细胞转化，其可能通过上调Runx2因子表达发挥促成骨作用。
关键词：诱导分化；骨髓间充质干细胞；17β-雌二醇；Ⅰ型胶原；Runx2
doi:10.3969/j.issn.1673-8225.2012.10.003

关键词: 诱导分化, 骨髓间充质干细胞, 17&beta, -雌二醇, 型胶原, Runx2

Abstract:

BACKGROUND: The estrogen receptor exists in bone marrow mesenchymal stem cells and estrogen promotes bone formation by regulating the proliferation and differentiation of bone marrow mesenchymal stem cells.
OBJECTIVE: To explore the effects of 17β-estradiol on the osteogenic differentiation of bone marrow mesenchymal stem cells (MSCs) and the underlying mechanism.
METHODS: MSCs were separated, purified, cultured and expanded. Different concentrations of 17β-estradiol was administrated to MSCs for some period. The level of type I collagen in culture medium was detected by Elisa method, and the expression of Runx2 mRNA and protein was analyzed by RT-PCR or Western blotting methods, respectively.
RESULTS: Compared with the control group, type I collagen expression in the MSCs was significantly increased on the 5th day after administration of 0.001, 0.01, 0.1 nmol/L 17β estradiol (P < 0.05) and it maintained high expression on the 7th day (P < 0.05). Runx2 mRNA expression increased in a dose-dependent manner on the 5th and 7th days after administration of 17 β-estradiol
(P < 0.05). These findings suggest that 17β-estradiol can induce the osteogenic differentiation of MSCs, which occurs possibly by upregulation of Runx2 expression.

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