中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (8): 1367-1370.doi: 10.3969/j.issn.1673-8225.2012.08.009

• 细胞外基质材料 extracellular matrix materials • 上一篇    下一篇

去细胞坐骨神经天然支架的成分分析★

曹荣龙，李长宇，张相彤   

  1. 哈尔滨医科大学附属第一临床医学院神经外科，黑龙江省哈尔滨市  150001
  • 收稿日期:2011-07-21 修回日期:2011-11-26 出版日期:2012-02-19 发布日期:2012-02-19
  • 通讯作者: 张相彤，教授，主任医师，硕士生导师，哈尔滨医科大学附属第一临床医学院神经外科，黑龙江省哈尔滨市 150001 zgxgtg@ sina.com.cn
  • 作者简介:曹荣龙★，男，1985年生，山东省沂水县人，在读硕士，主要从事坐骨神经去细胞支架在中枢神经损伤中的应用研究。 caoronglong241@163.com

Component analysis of a natural acellular sciatic nerve scaffold 

Cao Rong-long, Li Chang-yu, Zhang Xiang-tong   

  1. Department of Neurosurgery, the First Clinical College of Harbin Medical University, Harbin  150001, Heilongjiang Province, China
  • Received:2011-07-21 Revised:2011-11-26 Online:2012-02-19 Published:2012-02-19
  • Contact: Zhang Xiang-tong, Professor, Chief physician, Master’s supervisor, Department of Neurosurgery, the First Clinical College of Harbin Medical University, Harbin 150001, Heilongjiang Province, China zgxgtg@sina.com.cn
  • About author:Cao Rong-long★, Studying for master’s degree, Department of Neurosurgery, the First Clinical College of Harbin Medical University, Harbin 150001, Heilongjiang Province, China caoronglong241@ 163.com

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摘要：

背景：目前周围神经去细胞的方法较多，应用较多的是化学萃取法和液氮冻融法，但是化学萃取法耗时较长，而液氮冻融法则去除细胞不彻底。作者在查阅大量文献和多次实践的基础上，最终确定了冻融+化学萃取+机械振荡的优化法对坐骨神经进行脱细胞处理。
目的：对优化法制备的去细胞坐骨神经天然支架进行形态学分析，并对其成分进行鉴定。
方法：取Wistar大鼠双侧坐骨神经，随机分为2组，对照组不做特殊处理，实验组用冻融+化学萃取+机械振荡的优化法进行脱细胞处理。
结果与结论：用优化法制备的去细胞坐骨神经天然支架呈三维管状立体结构，许旺细胞、髓鞘及轴突去除完全。经免疫组化鉴定，Ⅰ型、Ⅲ型胶原和层粘连蛋白等细胞外基质主要成分得到保留。

关键词: 坐骨神经, 去细胞, 支架, 细胞外基质, 成分

Abstract:

BACKGROUND: Currently, there are so many acellular methods used in the peripheral nerve, and two common methods are chemical extraction and frozen-thawing method. However, chemical extraction has the disadvantage of time-consuming and frozen-thawing method cannot achieve complete cell-free. Freeze-thaw+chemical extraction+mechanical vibration were chosen as optimization method for acellular processing of sciatic nerve in this study based on a large amount of literatures and repeated practice.
OBJECTIVE: To analyze the morphology and composition of the natural acellular sciatic nerve scaffold prepared by optimization method.
METHODS: Bilateral sciatic nerves from Wistar rats were randomly divided into two groups. The control group did not receive special treatment. The experimental group was treated with freeze-thaw+chemical extraction+mechanical vibration to prepare acellular scaffold.
RESULTS AND CONCLUSION: Schwann cells, myelin sheaths and axons were removed completely from the optimized acellular sciatic nerve scaffold. And the scaffold showed a three-dimensional tubular structure. Extracellular matrixes of type Ⅰ collagen, type Ⅲ collagen and laminin were retained.

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