中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (8): 1331-1335.doi: 10.3969/j.issn.1673-8225.2012.08.001

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials •    下一篇

聚左旋乳酸/壳聚糖纳米纤维三维多孔支架复合骨髓间充质干细胞修复兔骨缺损**★△

王  欢1, 2，林宏生1, 2，查振刚1, 2，谭文成3△，郑力恒4△，姚  平5，张嘉晴6，苗贵强7   

  1. 1暨南大学骨科疾病研究所，广东省广州市510632； 2暨南大学附属第一医院骨科，广东省广州市
    510630；3澳门仁和医疗中心，澳门特别行政区
    999078；4澳门仁伯爵医院，澳门特别行政区
    999078；暨南大学医学院，5生理学教研室，6生物化学教研室，广东省广州市
    510632；7佛山市南海区人民医院，广东省佛山市
    528200
  • 收稿日期:2011-11-20 修回日期:2011-12-14 出版日期:2012-02-19 发布日期:2012-02-19
  • 通讯作者: 林宏生，教授，博士生导师，暨南大学骨科疾病研究所，广东省广州市510632；暨南大学附属第一医院骨科，广东省广州市 510630tlinhsh@jnu.cn
  • 作者简介:王欢★，男，1986年生，安徽省明光市人，暨南大学在读硕士，主要从事关节及脊柱外科与组织工程方面的研究。 illiyu@qq.com
  • 基金资助:

    国家863高技术研究发展计划资助项目 (2007AA09Z440)；澳门科学技术发展基金(031/2009/A)。

Three-dimensional porous poly(L-lactic acid)/chitosan nanofiber scaffolds combined with bone marrow mesenchymal stem cells in repairing of bone defects

Wang Huan1, 2, Lin Hong-sheng1, 2, Zha Zhen-gang1, 2, Tan Wen-cheng3△, Zheng Li-heng4△, Yao Ping5, Zhang Jia-qing6,
Miao Gui-qiang7   

  1. 1Institute of Bone Disease, Jinan University, Guangzhou  510632, Guangdong Province, China; 2Department of Orthopedics, the First Affiliated Hospital of Jinan University, Guangzhou  510630, Guangdong Province, China; 3Renhe Medical Center of Macao, Macao Special Administrative Region  999078, China; 4Hospital Conde S. of Macao, Macao Special Administrative Region  999078, China; 5Department of Physiology,      6Department of Biochemistry, Jinan  University Medical School, Guangzhou  510632, Guangdong Province, China;    7People's Hospital of Nanhai District of Foshan, Foshan  528200, Guangdong Province, China
  • Received:2011-11-20 Revised:2011-12-14 Online:2012-02-19 Published:2012-02-19
  • Contact: Lin Hong-sheng, Professor, Doctoral supervisor, Institute of Bone Disease, Jinan University, Guangzhou 510632, Guangdong Province, China; Department of Orthopedics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China tlinhsh@jnu.cn
  • About author:Wang Huan★, Studying for master’s degree, Institute of Bone Disease, Jinan University, Guangzhou 510632, Guangdong Province, China; Department of Orthopedics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China illiyu@qq.com
  • Supported by:

    National 863 High Technology Research and Development Program of China, No.2007AA09Z440*; Macao Science and Technology Development Foundation, No.031/2009/A*

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摘要：

背景：骨髓间充质干细胞具有向多种间质细胞谱系分化的能力，且支架材料的性能对骨缺损的修复有重要影响。
目的：观察聚左旋乳酸/壳聚糖纳米纤维三维多孔支架复合骨髓间充质干细胞治疗骨缺损。
方法：对骨缺损模型兔分别采用空白植入、髂后上棘自体松质骨移植、聚左旋乳酸/壳聚糖纳米纤维多孔支架移植和复合了骨髓间充质干细胞的聚左旋乳酸/壳聚糖纳米纤维多孔支架移植修复缺损部位。
结果与结论：至移植12周，移植复合了骨髓间充质干细胞的聚左旋乳酸/壳聚糖纳米纤维多孔支架的实验兔的缺损处有骨组织生成，支架材料降解，已完成缺损修复，其修复情况接近松质骨组；髂后上棘自体松质骨移植的实验兔的缺损修复完好，新形成的骨组织较规则；只植入聚左旋乳酸/壳聚糖纳米纤维多孔支架的实验兔有少量骨组织形成，材料部分降解；空白植入的实验兔缺损处无新生骨组织生成，主要由纤维结缔组织填充。说明新型的生物支架材料聚左旋乳酸/壳聚糖纳米纤维三维多孔支架与来源于新西兰大白兔的骨髓间充质干细胞复合培养后，植入同种异体兔股骨髁缺损处，使骨缺损的修复速度加快，表现为较好的体内诱导成骨的作用。

关键词: 纳米纤维, 聚左旋乳酸, 骨髓间充质干细胞, 骨缺损, 骨组织工程

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) have the ability to differentiate to a variety of mesenchymal cell lineages, and the properties of scaffold material have a great impact on bone defects.
OBJECTIVE: To observe the possibility of repairing bone defects by three-dimensional porous poly(L-lactic acid)/chitosan nanofiber (PLLA/CSNF) scaffolds combined with BMSCs.
METHODS: The rabbit bone defect models were implanted with posterior superior iliac spine autogenous cancellous bone (cancellous bone group), PLLA/CSNF porous scaffold and the PLLA/CSNF porous scaffold combined with BMSCs (BMSCs+PLLA/CSNF group). The model group was without implantation.   
RESULTS AND CONCLUSION: Bone tissue was formed in the defected position in the BMSCs+PLLA/CSNF group after implanted for 12 weeks, the scaffolds were degraded and the repair of the defect was completed, the effect of repairing defects was similar to that in the cancellous bone group; in the cancellous bone group, the defects were healing well and the newly formed bone tissue were arranged in order; some new bone tissue were formed and the scaffolds were degraded partly in PLLA/CSNF group while the defect was mainly filled with fibrous connective tissue in model group and there was no new bone tissue formation. The three-dimensional porous PLLA/CSNF scaffolds co-cultured with BMSCs implanted into the defects of the femoral condyle could increase the speed of repairing and possesses a superior osteoinductivity in vivo.

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