中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (7): 1184-1187.doi: 10.3969/j.issn.1673-8225.2012.07.011

• 血管组织构建 vascular tissue construction • 上一篇    下一篇

黄芪多糖胶原干预周围组织中核因子κB与血管内皮细胞生长因子表达而
促进毛细血管新生**★

周  勇1，姚  昶2，高  峰1，高卫卫1，陈  运2，缪雪华2   

  1. 1南京中医药大学，江苏省南京市  210029；2南京中医药大学附属医院普外科，江苏省南京市  210029
  • 收稿日期:2011-07-19 修回日期:2011-11-05 出版日期:2012-02-12 发布日期:2012-02-12
  • 通讯作者: 姚昶，博士，主任中医师，南京中医药大学附属医院普外科，江苏省南京市 210029 yaochang67@yahoo.com
  • 作者简介:周勇★，男，1987年生，汉族，南京中医药大学在读硕士，主要从事创伤修复的研究。
  • 基金资助:

    江苏省自然基金(BK2008491)；江苏省中医药领军人才基金(LJ200902)。

Enhancing angiogenesis by improving the expression of vascular endothelial growth factor and nuclear factor kappa B in surrounding tissues induced by astragalus polysaccharides collagen

Zhou Yong1, Yao Chang2, Gao Feng1, Gao Wei-wei1, Chen Yun2, Mou Xue-hua2   

  1. 1Nanjing University of Traditional Chinese Medicine, Nanjing  210029, Jiangsu Province, China; 2Department of General Surgery, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing  210029, Jiangsu Province, China
  • Received:2011-07-19 Revised:2011-11-05 Online:2012-02-12 Published:2012-02-12
  • Contact: Yao Chang, Doctor, Chief physician, Department of General Surgery, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China yaochang67@yahoo.com
  • About author:Zhou Yong★, Studying for master’s degree, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China
  • Supported by:

    Natural Science Foundation of Jiangsu Province, No. BK2008491*; Fund for Leading Talents of Chinese Medicine in Jiangsu Province, No. LJ200902*

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摘要：

背景：补气生血类中药具有与生长因子促血管生成作用相近的功效。
目的：观察黄芪多糖胶原促血管新生的疗效，及其对血管内皮细胞生长因子基因表达的核因子κB通路的影响。
方法：采用大鼠背部皮下植入模型，分别将黄芪多糖胶原和空白胶原植入大鼠背部两侧，于植入后3，7，14，21 d处死大鼠，取胶原周围肉芽组织进行检测。
结果与结论：造模后3 d时，大鼠肉芽组织中微血管数开始增加，7~14 d时达到峰值，之后下降。在造模后3，7，14 d，植入黄芪多糖胶原的大鼠周围肉芽组织中毛细血管数、血管内皮细胞生长因子mRNA及核因子κB蛋白的表达均显著高于植入空白胶原的大鼠(P < 0.01)，且核因子κB蛋白的表达早于毛细血管新生与血管内皮细胞生长因子mRNA的表达。说明黄芪多糖胶原促血管新生作用有可能是通过活化核因子κB信号通路而上调血管内皮细胞生长因子mRNA表达实现的。

关键词: 黄芪多糖, 胶原, 血管新生, 核因子&kappa, B, 血管内皮细胞生长因子

Abstract:

BACKGROUND: Lots of traditional Chinese medicines with the effects of nourishment and angiogenesis have the similar functions with those of angiogenic growth factors.
OBJECTIVE: To explore the angiogenic mechanism of astragalus polysaccharides (APS) collagen sponge by its regulating expression of vascular endothelial growth factor (VEGF) mRNA and the up signal pathway of nuclear factor κB (NF-κB) protein.
METHODS: Collagen sponges with and without APS immobilization were implanted inside subcutaneous pockets for 3, 7, 14 and 21 days respectively. The surrounding granulation tissue of collagen was taken to detect.
RESULTS AND CONCLUSION: The number of miocrovessels in granulation tissue was increased at 3 days after modeling and reached to peak at 7 to 14 day, and then decreased gradually. The number of miocrovessels in granulation tissue and the expressions of VEGF-mRNA and NF-κB protein of surrounding tissues of collagen sponges with APS immobilization were higher than that without APS immobilization (P < 0.01). The protein expression of NF-κB was earlier than the expression of VEGF-mRNA and newborn miocrovessels. The partly angiogenic mechanisms of collagen sponges with APS may be realized through the activation of the NF-κB protein pathway to improve VEGF-mRNA expression in the surrounding tissues.

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