中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (7): 1145-1149.doi: 10.3969/j.issn.1673-8225.2012.07.002

• 骨组织构建 bone tissue construction • 上一篇    下一篇

骨组织块法和酶消化法联合培养人成骨细胞★

陈  洁1，兰由玉1，何成松1，赵  毅2，刘  毅2   

  1. 1泸州医学院附属医院风湿血液科，四川省泸州市  646000；2四川大学华西医院风湿免疫科，四川省成都市  610041
  • 收稿日期:2011-07-05 修回日期:2011-11-03 出版日期:2012-02-12 发布日期:2012-02-12
  • 通讯作者: 刘毅，博士，教授，四川大学华西医院风湿免疫科，四川省成都市 610041 yi2006liu@163.com
  • 作者简介:陈洁★，女，1979年生，四川省自贡市人，汉族，2010年泸州医学院毕业，硕士，主治医师，主要从事风湿免疫性疾病的诊治研究。 cjlion@qq.com

Human osteoblasts co-cultured with bone tissue and enzymatic digestion method in vitro

Chen Jie1, Lan You-yu1, He Cheng-song1, Zhao Yi2, Liu Yi2   

  1. 1Department of Rheumatology and Hematology, the Affiliated Hospital of Luzhou Medical College, Luzhou  646000, Sichuan Province, China; 2Department of Rheumatology and Immunology, West China Hospital of Sichuan University, Chengdu  610041, Sichuan Province, China
  • Received:2011-07-05 Revised:2011-11-03 Online:2012-02-12 Published:2012-02-12
  • Contact: Liu Yi, Doctor, Professor, Department of Rheumatology and Immunology, West China Hospital of Sichuan University, Chengdu 610041, Sichuan Province, China i2006liu@163.com
  • About author:Chen Jie★, Master, Attending physician, Department of Rheumatology and Hematology, the Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China cjlion@qq.com

PDF

327

被引次数

9

摘要：

背景：成骨细胞培养难度大，不同培养方法得到的成骨细胞数量、纯度、增殖及分化活性各有区别。
目的：探讨理想的人成骨细胞体外培养的方法。
方法：取人髂骨松质骨，同时结合骨组织块法和酶消化法分离培养人成骨细胞。
结果与结论：分离培养的人成骨细胞生长状态良好，纯度较高。细胞贴壁生长，形态多样化，多呈多边形、纺锤型、梭形、三角形，胞浆丰富向外伸展出生长突，具有典型的成骨细胞形态特征；细胞增殖良好，碱性磷酸酶染色及矿化结节茜素红染色阳性。说明联合骨组织块法和酶消化法可成功分离培养人成骨细胞，是进行人成骨细胞体外培养较为理想的方法。
关键词：成骨细胞；原代培养；碱性磷酸酶；组织块法；酶消化法
doi:10.3969/j.issn.1673-8225.2012.07.002

关键词: 成骨细胞, 原代培养, 碱性磷酸酶, 组织块法, 酶消化法

Abstract:

BACKGROUND: It is difficult to culture osteoblasts, and different in cell number, purity, proliferation and differentiation activity obtained by different culture methods.
OBJECTIVE: To investigate the ideal culturing method of the human osteoblasts in vitro. 
METHODS: Human osteoblasts were isolated from trabecular bone of ilium by bone tissue and enzymatic digestion methods.
RESULTS AND CONCLUSION: The isolated and cultured human osteoblasts were in good condition and had a high purity. The cells were adherent growth, diversification in morphology, and mostly showed polygonal, spindle-shaped, triangular, and the cytoplasm was abundant and the spurt was stretched out. It has the typical characteristics of osteoblasts. The cells proliferation was well, alkaline phosphatase staining and mineralized nodules alizarin red staining was positive. We successfully cultured osteoblasts in vitro from trabecular bone of ilium by bone tissue and enzymatic digestion methods; it indicated that was the ideal method to culture osteoblasts.

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