中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (6): 1041-1045.doi: 10.3969/j.issn.1673-8225.2012.06.021

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

荧光原位杂交技术在异基因造血干细胞移植监测中的作用**★

莫耀禧1，周瑞莲2，莫伟英1，蓝  梅2，林金盈2   

  1. 广西壮族自治区人民医院，1生殖医学与遗传中心，2血液内科，广西壮族自治区南宁市  530021
  • 收稿日期:2011-05-13 修回日期:2011-07-04 出版日期:2012-02-05 发布日期:2012-02-05
  • 通讯作者: 林金盈，主任医师，硕士，广西壮族自治区人民医院血液科，广西壮族自治区南宁市 530021 jinyinglin@sina.com
  • 作者简介:莫耀禧★，男，1977年生，广西壮族自治区南宁市人，汉族，2007年大连医科大学毕业，硕士，助理研究员，主要从事分子生物学、细胞遗传学等方面的研究。moson1_ok@yahoo.com.cn 并列第一作者：周瑞莲★，女，1980年生，山东省诸城市人，汉族，2008年吉林大学第一临床医学院毕业，硕士，主治医师，主要从事白血病的诊断治疗等方面的研究。qianzilian117@163.com
  • 基金资助:

    广西壮族自治区卫生厅课题(Z2008057，Z2009133)。

Roles of fluorescence in situ hybridization in allogeneic hematopoietic stem cell transplantation

Mo Yao-xi1, Zhou Rui-lian2, Mo Wei-ying1, Lan Mei2, Lin Jin-ying2   

  1. 1Reproductive Medicine and Genetic Center, 2Department of Hematology, the People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-05-13 Revised:2011-07-04 Online:2012-02-05 Published:2012-02-05
  • Contact: Lin Jin-ying, Master, Chief physician, Department of Hematology, the People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, Guangxi Zhuang Autonomous Region, China jinyinglin@sina.com
  • About author:Mo Yao-xi★, Master, Research assistant, Reproductive Medicine and Genetic Center, the People’s Hospital of Guangxi Zhuang autonomous Region, Nanning 530021, Guangxi Zhuang Autonomous Region, China moson1_ok@yahoo.com.cn Zhou Rui-lian, Master, Attending physician, Department of Hematology, the People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, Guangxi Zhuang Autonomous Region, China qianzilian117@163.com
  • Supported by:

     the Health Bureau of Guangxi Zhuang Autonomous Region, No. Z2008057*, Z2009133*

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摘要：

背景：异基因造血干细胞移植后，嵌合率下降与移植物被排斥及白血病复发密切相关，常规骨髓细胞学检查、染色体检查、流式细胞学检查等均不能敏感特异地监测嵌合率。
目的：验证荧光原位杂交技术检测异基因造血干细胞移植后嵌合率的敏感性和特异性。
方法：应用荧光原位杂交法检测异性同胞异基因造血干细胞移植后患者X、Y 性染色体及慢性粒细胞白血病序列特异性bcr/abl融合基因，以骨髓细胞学检查为对照。
结果与结论：3例异基因造血干细胞移植患者共复查骨髓细胞学检查及荧光原位杂交检查7次。4次骨髓细胞学检查及荧光原位杂交检查均完全相符，另外3次骨髓细胞学检查提示完全缓解，而荧光原位杂交检查能检测到少量异常信号，证实应用荧光原位杂交法较骨髓细胞学具有显著优越性，能动态敏感、特异地检测嵌合率。
关键词：荧光原位杂交；白血病；异基因；造血干细胞；细胞移植
doi:10.3969/j.issn.1673-8225.2012.06.021

关键词: 荧光原位杂交, 白血病, 异基因, 造血干细胞, 细胞移植

Abstract:

BACKGROUND: The chimeric rate is very important in the allogeneic hematopoietic stem cell transplantation. Its decline always means the host anti-graft or leukemia relapse. Many techniques, including bone marrow cytology, conventional cytogenetic and flow cytometry, are not sensitive and specific enough in monitoring the chimerism.
OBJECTIVE: To verify the sensitivity and specificity of fluorescence in situ hybridization (FISH) in the detection of allogeneic chimerism after allogeneic hematopoietic stem cell transplantation.
METHODS: The chimerism of the sex mismatched Allo-HSCT was detected by FISH, utilizing X, Y chromosomes and bcr/abl fusion gene, in comparison with bone marrow cytology.
RESULTS AND CONCLUSION: Through monitoring the chimerism by FISH in 3 cases, which were sex mismatched Allo-HSCT, it is confirmed that FISH is more sensitive and specific than bone marrow cytology, and has the unique advantage in detection of chimerisms.

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