中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (6): 981-984.doi: 10.3969/j.issn.1673-8225.2012.06.007

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

乙醇改变骨髓间充质干细胞神经肽的表达*★

王义生1，朱绍阳1，李月白2，赵国强3，张战峰1   

  1. 1郑州大学第一附属医院骨科，河南省高等学校临床医学重点学科开放实验室，河南省郑州市 450052；郑州大学基础医学院，2生物化学教研室，3微生物免疫教研室，河南省郑州市 450001
  • 收稿日期:2011-11-28 修回日期:2011-12-20 出版日期:2012-02-05 发布日期:2012-02-05
  • 作者简介:王义生★，男，1951年生，河南省新乡市人，汉族，1982年河南医学院毕业，硕士，教授，主任医师，博士生导师，主要从事骨坏死的发病机制与防治，关节脊柱外科方面的研究。 wangyisheng@zzu.edu.cn
  • 基金资助:

    国家自然科学基金资助项目(81171776)，课题名称：hCGRPα基因修饰BMSCs联合自体骨移植治疗股骨头坏死的实验研究。

Effects of alcohol on the expression of neuropeptide in human bone marrow mesenchymal stem cells  

Wang Yi-sheng1, Zhu Shao-yang1, Li Yue-bai2, Zhao Guo-qiang3, Zhang Zhan-feng1   

  1. 1Departmemt of Orthopedics, the First Affiliated Hospital of Zhengzhou University, Open Laboratory of Key Subjects of Clinical Medicine of Key Subjects, Zhengzhou  450052, Henan Province, China; 2Department of Biochemistry; 3Department of Microbiology & Immunology, Basic Medical College of Zhengzhou University, Zhengzhou  450001, Henan Province, China
  • Received:2011-11-28 Revised:2011-12-20 Online:2012-02-05 Published:2012-02-05
  • About author:Wang Yi-sheng★, Master, Professor, Chief physician, Doctoral supervisor, Departmemt of Orthopedics, the First Affiliated Hospital of Zhengzhou University, Open Laboratory of Key Subjects of Clinical Medicine of Henan Province, Zhengzhou 450052, Henan Province, China wangyisheng@zzu.edu.cn
  • Supported by:

    the National Natural Science Foundation of China, No.81171776*

PDF

317

被引次数

5

摘要：

背景：研究发现骨髓间充质干细胞的分化受神经系统调控。
目的：观察乙醇对人骨髓间充质干细胞降钙素基因相关肽受体、P物质受体、过氧化物酶体增殖子活化受体γ mRNA及Runx2 mRNA表达的影响。
方法：将第2代人骨髓间充质干细胞随机分组培养：实验组加入0.09 mol/L乙醇，对照组正常培养。
结果与结论：培养11 d后，实验组降钙素基因相关肽受体、P物质受体、Runx2 mRNA表达较对照组明显降低(P < 0.01)，过氧化物酶体增殖子活化受体γ mRNA水平较对照组明显增高(P < 0.01)。表明乙醇改变了骨髓间充质干细胞中神经肽类物质的表达，减弱了其向成骨细胞方向分化。

关键词: 乙醇, 神经肽, 人源性骨髓间充质干细胞, 降钙素基因相关肽, P物质

Abstract:

BACKGROUND: Studies have found that the nervous system can effect the differentiation of bone marrow mesenchymal stem cells (BMSCs).
OBJECTIVE: To investigate the effect of alcohol on the expressions of calcitontin gene-related peptide receptor (CGRPR) mRNA, substance P receptor (SPR) mRNA, peroxisom proliferator-activeted receptor-γ (PPARγ) mRNA and Runx2 mRNA in human BMSCs. 
METHODS: The second generation of BMSCs were divided into two groups at random. The cells in experimental group were treated with 0. 09 mol/L alcohol, and the control group was not treated.
RESULTS AND CONCLUSION: The expressions of FGF mRNA, CGRPR mRNA, SPR mRNA and Runx2 mRNA in the experimental group were significantly lower those that in the control group (P < 0.01), but the expression of PPARγ mRNA was increased compared with control group (P < 0.01). Alcohol could change the expressions of neuropeptide and depress the differentiation to osteoblasts.    

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