中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (2): 367-371.doi: 10.3969/j.issn.1673-8225.2012.02.040

• 组织构建临床实践 clinical practice in tissue construction • 上一篇    下一篇

三磷酸腺苷结合盒转运子A1基因rs2066715多态性与青少年汉族人群的血脂水平

曹小丽1，尹瑞兴2，秦娇琴1，罗  曼1   

  1. 广西医科大学第一附属医院，1神经内科，2心血管内科，心血管病研究所，广西壮族自治区南宁市 530021
  • 收稿日期:2011-09-15 修回日期:2011-09-23 出版日期:2012-01-08 发布日期:2012-01-08
  • 作者简介:曹小丽，女，1979年生，湖北省黄冈市人，汉族，2001年毕业于武汉大学，主治医师，主要从事脑血管疾病的基础和临床研究。 maten1996@gmail.com

Association of adenosine triphosphate binding cassette transporter A1 gene rs2066715 polymorphism with blood lipid levels in young adults of Han populations

Cao Xiao-li1, Yin Rui-xing2, Qin Jiao-qin1, Luo Man1   

  1. 1Department of Neurology, 2Department of Cardiology, Institute of Cardiovascular Diseases, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-09-15 Revised:2011-09-23 Online:2012-01-08 Published:2012-01-08
  • About author:Cao Xiao-li, Attending physician, Department of Neurology, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China maten1996@gmail.com

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摘要：

背景：三磷酸腺苷结合盒转运子A1基因多态性与血脂水平及冠状动脉性心脏病相关，但对血脂的影响在不同种族、不同性别及年龄的人群研究当中结论有所不同。
目的：分析三磷酸腺苷结合盒转运子A1基因rs2066715 单核苷酸多态性位点与青年汉族人群血脂水平的关系。
方法：采用分层抽样，聚合酶链反应-限制性片段长度多态性方法，对394例35岁以下汉族青少年进行三磷酸腺苷结合盒转运子A1基因rs2066715位点检测，比较不同基因型与血脂水平的关系以及基因与环境之间的相互作用。
结果与结论：汉族青年人群三磷酸腺苷结合盒转运子A1基因rs2066715位点A等位基因携带率显著高于欧裔人群；全部检测人群中GG型占33.50%, GA 型占48.98%, AA 型占15.72%。不同性别三种基因型频率差异无显著性意义(P > 0.05)；A等位基因携带者高密度脂蛋白胆固醇及载脂蛋白A1水平低于非携带者(P < 0.05)。提示青年汉族人群A等位基因携带率高，且三磷酸腺苷结合盒转运子A1基因A等位基因携带者血浆高密度脂蛋白胆固醇及载脂蛋白A1水平低下。
关键词：三磷酸腺苷结合盒转运子；单核苷酸多态性；青年；血脂水平；基因型
doi:10.3969/j.issn.1673-8225.2012.02.040

关键词: 三磷酸腺苷结合盒转运子, 单核苷酸多态性, 青年, 血脂水平, 基因型

Abstract:

BACKGROUND: Adenosine triphosphate (ATP) binding cassette transporter A1 gene rs2066715 polymorphism is related to blood lipid levels and coronary heart disease. However, the effects on blood lipid levels vary by race, age and gender in the previous researches.
OBJECTIVE: To investigate the association of rs2066715 single-nucleotide polymorphism of ATP binding cassette transporter A1 gene with blood lipid levels in young adults of Han populations.
METHOUDS: A total of 394 young adults of Han populations under the age of 35 were included. Genotypes of ATP binding cassette transporter A1 gene rs2066715 site were determined by stratified sampling and polymerase chain reaction-restriction fragment length polymorphism in all subjects. The association of different genotypes with blood lipid levels and the interaction between gene and environment were evaluated.
RESULTS AND CONCLUSION: The carrier rate of gene site A in ATP binding cassette transporter A1 gene rs2066715 among young adults of Han populations were significantly higher than that of European populations. There were no significant differences in the carrier rate of the three genotypes between genders (P > 0.05). The carrier rate of GG, GA and AA genotypes were 33.50%, 48.98% and 15.72%, respectively. The levels of high-density lipoprotein cholesterol and apolipoprotein A-I were lower in carriers of the A allele than that of the non-carriers (P < 0.01). These findings indicate that the carrier rate of the A allele among young adults of Han populations is high. The level of high-density lipoprotein cholesterol and apolipoprotein A-I is low in the carriers of the A allele in ATP binding cassette transporter A1 gene.

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