中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (2): 211-214.doi: 10.3969/j.issn.1673-8225.2012.02.004

• 骨组织构建 • 上一篇    下一篇

大鼠腭中缝牵张成骨中骨形态发生蛋白7的作用

李  威1，李  鹍2，王培军1，韩晶莹1   

  1. 哈尔滨医科大学第二临床医学院，1 口腔正畸科，2 科研科，黑龙江省哈尔滨市150086
  • 收稿日期:2011-11-01 修回日期:2011-12-06 出版日期:2012-01-08 发布日期:2012-01-08
  • 通讯作者: 韩晶莹，哈尔滨医科大学在读博士，哈尔滨医科大学第二临床医学院，口腔正畸科，黑龙江省哈尔滨150086 jingying5787@163.com
  • 作者简介:李威★，男，1983年生，辽宁省人，汉族，哈尔滨医科大学在读硕士，医师，主要从事口腔正畸的临床诊疗工作。 15904611995@163.com
  • 基金资助:

    黑龙江省自然科学基金(D20965)；市科委青年科技创新人才基金资助项目(2010RFQXS113)。

Role of bone morphogenetic protein-7 in the distraction osteogenesis of rat mid-palatal suture

Li Wei1, Li Kun2, Wang Pei-jun1, Han Jing-ying1   

  1. 1Department ofOrthodontics,2Research Division,the Second AffiliatedHospital of HarbinMedical University,Harbin 150086,HeilongjiangProvince, China
  • Received:2011-11-01 Revised:2011-12-06 Online:2012-01-08 Published:2012-01-08
  • Contact: Han Jing-ying,Studying fordoctorate,Department ofOrthodontics, theSecond AffiliatedHospital of HarbinMedical University,Harbin 150086,HeilongjiangProvince, Chinajingying5787@163.com
  • About author:Li Wei★, Studying formaster’s degree,Physician,Department ofOrthodontics, theSecond AffiliatedHospital of HarbinMedical University,Harbin 150086,HeilongjiangProvince, China 15904611995@163.com
  • Supported by:


    theNatural ScienceFoundation ofHeilongjiangProvince, No.D20965*; the YouthFoundation for Technological
    Innovation of theMunicipal Committeeof Science andTechnology, No.2010RFQXS113*

PDF

452

被引次数

6

摘要：

背景：已有实验证实，骨形态发生蛋白7可诱导骨组织和软骨组织的形成，在牵张成骨的过程中促进骨组织形成。
目的：测定骨形态发生蛋白7在腭中缝牵张成骨中的作用及机械扩张力作用下的变化。
方法：采用自制双眼扩弓簧对大鼠施加扩张力，建立上颌骨扩张大鼠模型。分别于建模后第1，3，5，7，9，12，14天随机选取大鼠取腭中缝组织，实时定量PCR法测定目的基因的变化规律。
结果与结论：建模后第3，9，12，14天大鼠腭中缝组织骨形态发生蛋白7 mRNA的表达逐渐升高(P < 0.05)。结果证实，大鼠腭中缝牵张成骨时，骨形态发生蛋白7 mRNA在机械力刺激下表达明显上调。

关键词: 骨形态发生蛋白7, 牵张成骨, 大鼠, 腭中缝, 骨组织构建

Abstract:

BACKGROUND: Experiments have confirmed that bone morphogenetic protein-7 can induce bone and cartilage formation; it canalso promote bone formation in the process of distraction osteogenesis.
OBJECTIVE: To explore the role of bone morphogenetic protein-7 in the suture distraction osteogenesis of rat mid-palatal suture and to detect the changes of bone morphogenetic protein-7 under the mechanical expansion force.
METHODS: Expansionary force was applied on rats using a self-made expansing arch spring for binoculus to construct maxillary expansion rat model. Rat mid-palatal sutures were randomly collected on 1, 3, 5, 7, 9, 12 and 14 days after model construction;real-time PCR was performed to detect the changing regularities of the target genes.
RESULTS AND CONCLUSION: The expression of the bone morphogenetic protein-7 mRNA in the rat mid-palatal suturesincreased gradually on 3, 9, 12 and 14 days after model construction (P < 0.05). These findings demonstrate that bonemorphogenetic protein-7 mRNA expression is significantly increased in the distraction osteogenesis process of rat mid-palatalsutures under the stimulus of mechanical force.

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