中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (2): 206-210.doi: 10.3969/j.issn.1673-8225.2012.02.003

• 骨组织构建 • 上一篇    下一篇

骨形态发生蛋白2基因转染犬牙髓细胞

冯艳红1，刁志虹2，高  毅1，李  威1
  

  1. 1河北省中医院口腔科，河北省石家庄市  050011；  
    2石家庄市第一医院，河北省石家庄市  050011
  • 收稿日期:2011-11-02 修回日期:2011-11-10 出版日期:2012-01-08 发布日期:2012-01-08
  • 通讯作者: 高毅，硕士，主任医师，教授，河北省中医院口腔科，河北省石家庄市050011 dzh666888@yahoo.cn
  • 作者简介:冯艳红，女，1975年生，河北省赵县人，汉族，1996年白求恩军医学院毕业，主治医师，主要从事口腔内科方面的研究。 hbedzz@sohu.com 并列第一作者：刁志虹，副主任医师，主要从事口腔颌面外科方面的研究。 dzh666888@yahoo.cn
  • 基金资助:

    河北省科技厅科技支撑计划(JB00-09276102D-15)，BMP 基因克隆转染牙髓细胞复合异种烧结骨盖髓的实验研究

Ultrastructure changes of dog dental pulp cells transfected with bone morphogenetic proteins 2 in vitro

Feng Yan-hong1, Diao Zhi-hong2, Gao Yi1, Li Wei1   

  1. 1Department ofStomatology, HebeiProvincial Hospital ofTraditional ChineseMedicine,Shijiazhuang050011, HebeiProvince, China;
    2First Hospital ofShijiazhuang,Shijiazhuang050011, HebeiProvince, China
  • Received:2011-11-02 Revised:2011-11-10 Online:2012-01-08 Published:2012-01-08
  • Contact: Gao Yi, Master, Chiefphysician, Professor,Department ofStomatology, HebeiProvincial Hospital ofTraditional ChineseMedicine,Shijiazhuang050011, HebeiProvince, China dzh666888@yahoo.cn
  • About author:Feng Yan-hong,Attending physician,Department ofStomatology, HebeiProvincial Hospital ofTraditional ChineseMedicine,Shijiazhuang050011, HebeiProvince, China hbedzz@sohu.com Diao Zhi-hong,Associate chiefphysician, FirstHospital ofShijiazhuang,Shijiazhuang050011, HebeiProvince, China. dzh66888@yahoo.cn Feng Yan-hong andDiao Zhi-hong wereconsidered as co-firstauthors.
  • Supported by:

    Support Program ofScience andTechnologyDepartment of HebeiProvince, No.JB00-09276102D-15*

PDF

494

被引次数

10

摘要：

背景：研究发现骨形态发生蛋白2具有诱导间充质细胞向成骨细胞表型分化以及诱导新骨及修复性牙本质形成的能力。
目的：通过对细胞超微结构的分析，探讨骨形态发生蛋白2基因转染的犬牙髓细胞，向成牙本质细胞转化的过程。
方法：将培养的性状稳定的第4代犬牙髓细胞分为3组：基因转染组转染pEGFP-N1-BMP-2基因，空白载体转染组转染EGFP-N1空白荧光载体，未转染组不转染。
结果与结论：成功构建pEGFP-N1-BMP-2真核表达质粒，并成功将其转染犬牙髓细胞， pEGFP-N1-BMP-2质粒转染促进犬牙髓细胞分泌骨形态发生蛋白2。pEGFP-N1-BMP-2质粒转染有促进牙髓细胞碱性磷酸酶活性的作用。透射电镜观察结果显示：转染后的犬牙髓细胞具有成牙本质细胞的形态特点。说明pEGFP-N1-BMP-2 真核表达质粒转染后的牙髓细胞，具有成牙本质细胞的特征。

关键词: 骨形态发生蛋白2, 真核表达质粒, 基因转染, 牙髓细胞, 超微结构

Abstract:

BACKGROUND: The research has shown that bone morphogenetic protein 2 (BMP-2) gene can induce mesenchymal stem cellsdifferentiate into osteoblast phenotype and has the potential to the formation of new bone and reparative dentin.
OBJECTIVE: To investigate the progress of dog dental pulp cells (DDPCs) transfected with BMP-2 differentiate into odontoblastthrough the analysis of ultrastructure.
METHODS: The forth generation of DDPCs with stable traits was divided into three groups: Gene transfection group wastransfected with pEGFP-N1-BMP-2; Empty vector transfection group was transfected with EGFP-N1 empty fluorescence vector;Control group was without transfection.
RESULTS AND CONCLUSION: pEGFP-N1-BMP-2 eukaryotic expression plasmid was constructed successfully and transfectedwith DDPCs. pEGFP-N1-BMP-2 plasmid transfection could promote the secretion of BMP-2 in BMP2-DDPCs and the activity ofalkaline phosphatase of DDPCs. The transmission electron microscope observation showed that the transfected DDPCs had thephenotype characteristics of odontoblast. It indicates that the DDPCs transfected with pEGFP-N1-BMP-2 eukaryotic expressionplasmid has the characteristics of odontoblast.

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