中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (2): 201-205.doi: 10.3969/j.issn.1673-8225.2012.02.002

• 骨组织构建 • 上一篇    下一篇

人工虎骨粉干预大鼠成骨细胞增殖和Ⅰ型胶原的表达

韩  娜1，王天兵2，寇玉辉2，张殿英2，张培训2，殷晓峰2，姜保国2
  

  1. 北京大学人民医院，1临床分子生物学研究所，2创伤骨科，北京市100044
  • 收稿日期:2011-09-21 修回日期:2011-11-04 出版日期:2012-01-08 发布日期:2012-01-08
  • 通讯作者: 姜保国，博士，主任医师，博士生导师，北京大学人民医院创伤骨科，北京市 100044 jiangbaoguo@vip.sina.com
  • 作者简介:韩娜☆，女，1975年生，山东省淄博市人，汉族，2008年北京大学医学部毕业，博士，助理研究员，主要从事骨折愈合、骨代谢等方面的研究。 hanna@bjmu.edu.cn
  • 基金资助:

    国家自然科学基金(30973066、31040043)，首都医学发展科研基金(2007-2011)，北京市自然科学基金(7113170)，教育部博士点新教师基金(20090001120096)和北京大学人民医院研究与发展基金(RDB2010-30 )资助。

Effect of bionic tiger-bone powder on rat osteoblasts proliferation and typeⅠcollagenexpression

Han Na1, Wang Tian-bing2, Kou Yu-hui2, Zhang Dian-ying2, Zhang Pei-xun2, Yin Xiao-feng2, Jiang Bao-guo2   

  1. 1Institute of Clinical Molecular Biology;   2Department of Orthopaedics and Traumatology, People's Hospital of Peking University, Beijing  100044, China；
  • Received:2011-09-21 Revised:2011-11-04 Online:2012-01-08 Published:2012-01-08
  • Contact: Jiang Bao-guo, Doctor, Chief physician, Doctoral supervisor, Department of Orthopaedics and Traumatology, People's Hospital of Peking University, Beijing 100044, China jiangbaoguo@vip.sina.com
  • About author:Han Na☆, Doctor, Assistant researcher, Institute of Clinical Molecular Biology, People's Hospital of Peking University, Beijing 100044, China hanna@bjmu.edu.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30973066*, 31040043*; Fund of Capital Medical Development and Research, No. 2007-2011*; Beijing Natural Science Foundation, No. 7113170*; Doctoral Fund of Ministry of Education of China, No. 20090001120096*; Peking University People’s Hospital Research and Development Funds, No. RDB2010-30*

     

PDF

300

被引次数

11

摘要：

背景：人工虎骨粉主要用于骨质疏松症、骨折、类风湿关节炎等骨科疾病的治疗，并在临床中取得较好的疗效，但其成骨作用的药物机制尚未明确。
目的：观察人工虎骨粉对大鼠成骨细胞增殖和Ⅰ型胶原表达的影响。
方法：采用新生SD大鼠颅盖骨组织二次酶消化法培养原代成骨细胞，其中人工虎骨粉干预组细胞培养液的药物终质量浓度分别为10-4，10-5，10-6 g/L。对照组给予不含人工虎骨粉的无血清DMEM细胞培养液。
结果与结论：噻唑蓝比色法检测结果显示，人工虎骨粉可显著提高成骨细胞增殖活力(P < 0.05)，流式细胞仪检测结果显示，人工虎骨粉可提高S期和M/G2期细胞分布比例，并伴有G0/ G1期细胞比例降低；RT-PCR和ELISA的检测结果显示，各质量浓度人工虎骨粉处理成骨细胞48 h，均可以提高成骨细胞Ⅰ型胶原的表达(P < 0.05)。结果表明人工虎骨粉对体外培养的大鼠原代成骨细胞具有促增殖作用并可以促进Ⅰ型胶原的合成和分泌。

关键词: 人工虎骨粉, 成骨细胞, Ⅰ型胶原, 细胞增殖, 大鼠

Abstract:

BACKGROUND: Bionic tiger-bone powder is mainly used for the treatment of osteoporosis, fracture, rheumatoid arthritis and other bone diseases, and has achieved better clinical efficacy. However, the mechanisms of osteogenesis has not clear yet.
OBJECTIVE: To observe the effects of bionic tiger-bone powder on the proliferation of rat osteoblasts and the expression of typeⅠcollagen.
METHODS: We cultivate primary osteoblasts with secondary enzyme digestion of the newborn rat skull bone tissue. The Concentrations of culture medium in bionic tiger-bone powder intervention group were 10-4, 10-5 and 10-6 g / L. The control group was injected with the serum-free DMEM culture medium without bionic tiger-bone powder.  
RESULTS AND CONCLUSION: MTT colorimetric assay results showed that bionic tiger-bone powder could improve osteoblasts proliferation vitality apparently (P < 0.05). Meanwhile the proportion of S phase and M/G2 phase cells was increased but that of G0/G1 phase cells was decreased through flow cytometry. The RT-PCR and ELISA test showed that bionic tiger-bone powder could increase the expression of osteoblasts typeⅠcollagen (P < 0.05) when handling with them in the concentration of 10-6, 10-5 and 10-4 mg/ml for 48 hours. Bionic tiger-bone powder could promote the proliferation of rat primary osteoblasts in vitro, and could promote the synthesis and secretion of typeⅠcollagen.