中国组织工程研究

中国组织工程研究 ›› 0, Vol. ›› Issue (0): 111-115.doi: 10.3969/j.issn.1673-8225.2012.01.024

• 骨髓干细胞 • 上一篇    下一篇

微孔膜法比较Stro-1+和Stro-1-间充质干细胞的免疫调节作用

许  雯1，张翼鷟1，赵丹丹2，赵智刚1，于  泳1，赵伟鹏1
  

  1. 1天津医科大学附属肿瘤医院血液科，天津市“肿瘤防治”重点实验室，天津市300060； 2解放军总医院第一附属医院血液科，北京市  100037
  • 收稿日期:2011-07-01 修回日期:2011-08-11
  • 通讯作者: 张翼鷟，博士，副主任医师，天津医科大学附属肿瘤医院，天津市 300060 aprilzyz@yahoo.com
  • 作者简介:许雯★，女，1986年生，天津市人，天津医科大学在读硕士，主要从事血液肿瘤的临床与实验研究。 littleapple33@126.com

Comparison of immunomodulatory effects between Stro-1+ and Stro-1- mesenchymal stem cellsby micro-porous membrane

Xu Wen1, Zhang Yi-zhuo1, Zhao Dan-dan2, Zhao Zhi-gang1, Yu Yong1, Zhao Wei-peng1   

  1. 1Department of Hematology, Cancer Institute and Hospital of Tianjin Medical University, Tianjin  300060, China; 2Department of Hematology, First Affiliated Hospital of General Hospital of Chinese PLA, Beijing  100037, China
  • Received:2011-07-01 Revised:2011-08-11
  • Contact: Zhang Yi-zhuo, Doctor, Associate chief physician, Department of Hematology, Cancer Institute and Hospital of Tianjin Medical University, Tianjin 300060, China aprilzyz@yahoo.com
  • About author:Xu Wen★, Studying for master’s degree, Department of Hematology, Cancer Institute and Hospital of Tianjin Medical University, Tianjin 300060, China littleapple33@126.com

PDF

454

被引次数

9

摘要：

背景：研究表明间充质干细胞对淋巴细胞的增殖具有免疫抑制效应。但间充质干细胞是非均一性的细胞群，Stro-1+的抑制效应显著强于Stro-1-间充质干细胞。
目的：评估Stro-1+和Stro-1-间充质干细胞对淋巴细胞增殖抑制效应的显著差异在间充质干细胞与淋巴细胞非直接接触的情况下是否依然存在。
方法：先将1×104，3×104 Stro-1+或Stro-1-间充质干细胞加入微孔膜培养板(Transwell)的下室，一两小时待细胞贴壁后，再将1×104反应性外周血淋巴细胞和等量经照射的刺激性外周血淋巴细胞加入培养板的上室进行共孵育。培养5 d后以3H-TdR掺入率检测外周血淋巴细胞的增殖情况。对照组为间充质干细胞与淋巴细胞直接接触的培养体系。
结果与结论：在间充质干细胞与淋巴细胞直接接触的条件下，Stro-1+间充质干细胞对淋巴细胞增殖的免疫抑制效应显著强于Stro-1-间充质干细胞；当使用微孔膜培养板阻断间充质干细胞与外周血淋巴细胞的直接接触时，这一免疫抑制作用的显著差异则消失，并且不论是Stro-1+还是Stro-1-间充质干细胞对淋巴细胞增殖的抑制作用均显著弱于各亚群与外周血淋巴细胞直接接触的情况。提示间充质干细胞与淋巴细胞的直接接触是间充质干细胞的免疫调节效应得以发挥的主要因素，而某些可溶性因子在此过程中的作用只占小部分；细胞间的这种“直接对话”也是Stro-1+间充质干细胞优势性免疫调节作用得以发挥的前提。

关键词: 免疫抑制, Stro-1, 间充质干细胞, Transwell, 混合淋巴细胞反应

Abstract:

BACKGROUND: Mesenchymal stem cells (MSCs) have been proved to posses the immunosuppressive effect on proliferation of lymphocytes. However, MSCs have a feature of nonuniformity. In addition, the immunosuppressive effect of Stro-1+ MSCs is obviously stronger than that of Stro-1- MSCs.
OBJECTIVE: To investigate whether the significant difference in suppressive effect between Stro-1+ and Stro-1- MSCs still exists following the physical separation of MSCs from lymphocytes.
METHODS: 1×104 and 3×104 Stro-1+ or Stro-1- MSCs were seeded in the lower chamber of transwell plate for 1-2 hours in order to achieve cell attachment, and then 1×104 reactive peripheral blood lymphocytes (PBLs) were incubated with stimulating PBLs of the same amount in the upper chamber of the transwell plate. After 5 days of culture, proliferation of the PBLs was measured by 3H-TDR incorporation.
RESULTS AND CONCLUSION: The immunosuppressive effect of Stro-1+ MSCs was significantly stronger than that of Stro-1- MSCs when there was a direct connection between MSCs and PLBs. However, the immunosuppressive effects of the two subsets followed using transwell culture system were decreased than before. It is indicated that the direct connection between MSCs and PBLs may play a key role in the immunosuppressive effect of MSCs, and few soluble factors contribute a little during this. Furthermore, such “cross-talk” between cells is the prerequisite for MSCs to play the advantaged immune regulation

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