中国组织工程研究

中国组织工程研究 ›› 0, Vol. ›› Issue (0): 95-98.doi: 10.3969/j.issn.1673-8225.2012.01.020

• 骨髓干细胞 • 上一篇    下一篇

羊膜上皮细胞移植治疗脑缺血缺氧新生大鼠

刘红敏，陈旭东，华新宇
  

  1. 漯河医学高等专科学校组织胚胎学教研室，河南省漯河市  462000
     
  • 收稿日期:2011-05-17 修回日期:2011-07-08
  • 作者简介:刘红敏★，女，1972年生，河南省禹州市人，汉族，河南大学生物专业毕业，硕士，副教授，主要从事发育神经生物学的研究。 lhliuhongmin@yahoo.com.cn

Transplantation of human amniotic epithelial cells in treating neonatal rats withhypoxic-ischemic brain encephalopathy

Liu Hong-min, Chen Xu-dong, Hua Xin-yu
  

  1. Department of Histology and Embryology, Luohe Medical College, Luohe  462002, Henan Province, China
     
  • Received:2011-05-17 Revised:2011-07-08
  • About author:Liu Hong-min★, Master, Associate professor, Department of Histology and Embryology, Luohe Medical College, Luohe 462002, Henan Province, China lhliuhongmin@yahoo.com.cn

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340

被引次数

4

摘要：

背景：研究表明移植羊膜上皮细胞可改善神经功能，但其作用机制尚不清楚。
目的：观察缺氧缺血对新生大鼠脑神经干细胞和神经颗粒素表达的影响，及羊膜上皮细胞移植后对其治疗作用。
方法：建立缺氧缺血性脑损伤模型，46只SD大鼠随机抽签法分为3组，移植组和模型组缺血缺氧后经侧脑室分别注入1× 1012 L-1人羊膜上皮细胞悬液和PBS治疗；假手术组注射等量的生理盐水。
结果与结论：移植组大鼠神经功能较模型组明显改善，移植后第3周脑切片检出NSE阳性细胞存在，神经颗粒素在第3周的表达模型组低于假手术组，移植组较模型组增加；而nestin结果显示模型组较假手术组增加，移植组较模型组增加。提示羊膜上皮细胞移植入脑缺血缺氧大鼠脑内后，可减轻缺氧缺血后神经元的损伤，改善神经功能，其机制可能是注射入侧脑室的细胞促进自体神经干细胞的增殖分化和突触再生而实现的。

关键词: 人羊膜上皮细胞, 缺血缺氧性脑病, 神经颗粒素, 神经干细胞, 大鼠

Abstract:

BACKGROUND: The previous studies showed that transplantation of human amniotic epithelial cells (hAECs) could improve neurological function, but the mechanism is unclear.
OBJECTIVE: To explore the effects on neural stem cells and the expression of Neurogranin (Ng) of neonatal rats with hypoxic-ischemic encephalopathy, and the therapeutic effects of transplantation of human amniotic epithelial cells (hAECs).
METHODS: Forty-six rats were randomly divided into sham operation control, model group, transplantation group. The 1×1012/L hAECs and PBS were respectively injected into the cerebral ventricle of the transplantation group and model group, and sham operation group did not treated.
RESULTS AND CONCLUSION: Compared with model group, the neurological function was significantly improved in the transplantation group, NSE positive cells were detected in the transplantation group rat brains at 3 weeks following transplantation, Ng proteins expression in the model group was significantly lower than the sham operation group and the transplantation gorup at 3 weeks following transplantation. nestin proteins expression in the model group was higher than that in the sham operation group and lower than that in the transplantation group. Results indicated that hAECs injected into the cerebral ventricles could reduce the hypoxic-ischemic injury to neurons and improve neurological function. The mechanism was maybe achieved by autogenous neural stem cell proliferation, differentiation and synaptic regeneration stimulated by hAECs and its secretion.

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